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Investigating the Changes in the Antioxidant and Enzyme System of Litopenaeus Vannamei during Exposure to Silver Nitrate Texto completo
2022
Siyahooei, Abdolrazaq | Bita, Seraj | Ghasemzadeh, Javad
BACKGROUND: Oxidative stress is believed to be one of the major causes of tissue damage in aquatic animals exposed to heavy metals. It leads to certain changes in the antioxidant and enzyme system. Given the fact that research on the effect of sub-acute toxicity of silver nitrate in shrimps is not very developed, the present study can be conducive to formulating the international standards of contamination of shrimps with silver nitrate.OBJECTIVES: The present study aimed to evaluate the toxic effects of silver nitrate on the changes in the antioxidant and enzyme systems of hepatopancreas, muscle and gills of Litopenaeus vannamei.METHODS: After acclimatization of shrimps, they were exposed to silver nitrate with concentrations of 0.0084, 0.021, 0.042 and 0.063 mg/L for 21 days. At the end of the experimental period, gill, muscle and hepatopancreas were sampled, and the changes in the antioxidant system (SOD, GPx and GST) and metabolic enzymes (ALT, ALP, AST and LDH) were examined.RESULTS: There was no significant difference in terms of SOD and GST activity in the gill and muscle of the exposed shrimps (P>0.05). However, GPx in treatment 4 increased significantly in gill and muscle while it saw a decrease in hepatopancreas (P<0.05). In hepatopancreas, GST significantly increased in treatments 3 and 4 (P<0.05) whereas SOD did not show any significant changes compared with other treatments (P>0.05). The metabolic enzymes of the muscle did not show any significant differences in any of the treatments (P>0.05), but in gill, the level of ALT in treatment 4 decreased significantly while the levels of AST and LDH in treatment 3 and 4 significantly increased (P<0.05). In hepatopancreas, the activity of ALT in treatments 2 and 4, AST in treatments 3 and 4, and ALP in all treatment except treatment 1 saw significant reduction. Nevertheless, LDH in treatments 3 and 4 had a significant rise (P<0.05).CONCLUSIONS: A significant increase in GST and LDH as well as a significant decrease in GPx and ALT, AST and ALP enzymes in the hepatopancreas revealed that the antioxidant and enzyme system of shrimps is further disturbed with the rise in silver nitrate concentration in the hepatopancreas compared to the gill and muscle.
Mostrar más [+] Menos [-]Comparative study of leptospiral strains ictero No. 1 and RGA by restriction endonuclease DNA analysis.
1988
Hata K. | Yamaguchi T. | Ono E. | Yanagawa R.
Characteristics and application of monoclonal antibody to progesterone, 2; Development of progesterone enzyme-linked immunosorbent assay (ELISA).
1991
Kang C.B. | Kim J.S.
Effects of vitamin C and magnesium L-threonate treatment on learning and memory in lead-poisoned mice Texto completo
2021
Bislimi Kemajl | Mazreku Ilir | Halili Jeton | Aliko Valbona | Sinani Kushtrim | Hoxha Liridon
The aim of this study was to investigate the effects of vitamin C (vit C) and magnesium L-threonate (MgT) on the learning ability and memory of mice intoxicated with lead acetate.
Mostrar más [+] Menos [-]Proinsulin C-peptide induces c-Jun N-terminal kinase 1 expression in LEII mouse lung capillary endothelial cells
2009
Furuya, D.T., Hokkaido Univ., Sapporo (Japan) | Ishii, T. | Kamikawa, A. | Shimada, K. | Machado, U.F. | Saito, M.;Kimura | Kimura, K.
To characterize the roles of C-peptide in vascular homeostatic processes, we examined the genes regulated by C-peptide in LEII mouse lung microvascular endothelial cells. Treatment of the cells with C-peptide increased the expression of c-Jun N-terminal kinase 1 (JNK1) mRNA dose-dependently, accompanied by an increase in JNK1 protein content. Prior treatment of the cells with PD98059, an ERK kinase inhibitor or SB203580, a p38MAPK inhibitor, abrogated the C-peptide-elicited JNK1 mRNA expression. These results indicate that C-peptide increases JNK1 protein levels, possibly through ERK- and p38MAPK-dependent activation of JNK gene transcription.
Mostrar más [+] Menos [-]Blood serum chemistry and enzyme values of laboratory rabbits in absence and presence of dietary green.
2011
Gera, Sandeep | Guha, Anirban | Kapoor , P. K. | Chander, Suresh
The scarcity of green during summer months imposes nutritional stress on farm animals. In this study we examined the effect of nutritional stress on various biochemical parameters of rabbits.Control and green deprived groups each of 20, weaned New Zealand White rabbits, of either sex, were randomly placed and observed for two months. Then green was re-introduced in deprived group for again two months. Blood sera harvested at every 15th day and analyzed using RA 50 Chemistry auto analyzer. Significant (p0.05) decrease of alkaline phosphatase (AKP), lactate dehydrogenase (LDH) and increase in gamma glutamyl transpeptidase (GGT) and serum cholesterol was observed in rabbits green deprived group.
Mostrar más [+] Menos [-]Impacts of enzymes and probiotic in improving the utilization of sieved olive pulp meal in growing rabbit diets Texto completo
2024
Abd-Alfattah A. Alderey | Nabila E.M. El-Kassas | Eman A. Hussein | Soha A. Farag | Ayman A. Hassan | Safaa E.S. Atia | Manal H.A. Gomaa | Eman S. El-Hadad | Salma H. Abu Hafsa
Objective: This research assesses the utilization of sieved olive pulp (SOP) in the diet of growing rabbits through the use of an exogenous enzyme or dry yeast as a pretreatment. Materials and Methods: One hundred sixty-eight male V-Line rabbits aged 5 weeks (weighing 550 ± 25 gm) were randomly divided into seven groups with eight replicates each as follows: the control group was fed a basal diet without supplementation; while the other six groups were fed basal diets containing 20% and 25% of SOP and supplemented with 0.1 gm/kg Econase (E), 0.5 gm/kg dry yeast (Y), and a combination of both. The experiment lasted for 8 weeks. Results: The results indicated that supplementation of E, Y, and EY into rabbit diets containing SOP improved live body weight, body weight gain, feed conversion ratio, and nutrient digestibil¬ity. A higher dressing percentage was observed in the groups fed a 20% and 25% SOP diet sup¬plemented with EY (p < 0.05). The treated groups showed an increase in total protein, albumin, globulin, A/G ratio, total antioxidant capacity, superoxide dismutase, and glutathione peroxidase (p < 0.05), while there was a significant decrease in triglycerides, total cholesterol, and malondi¬aldehyde levels (p < 0.05) compared to the control. Rabbit groups fed an SOP diet supplemented with E, Y, or EY demonstrated higher (p < 0.05) economic efficiency compared to the control. Conclusion: Supplementing the diet of rabbits containing SOP with exogenous enzymes and/or dry yeast enhances the nutritional value of SOP while improving rabbit performance, nutrient digestibility, and antioxidant status. [J Adv Vet Anim Res 2024; 11(1.000): 161-170]
Mostrar más [+] Menos [-]Sunflower meal and supplementation of enzyme complex in diets for growing and finishing pigs Texto completo
2014
Wagner Azis Garcia de Araújo | Luis Fernando Teixeira Albino | Horacio Santiago Rostagno | Melissa Isabel Hannas | Jorge Armando Prada Luengas | Francisco Carlos de Oliveira Silva | Thony Assis Carvalho | Rosana Cardoso Maia
Sunflower meal and supplementation of enzyme complex in diets for growing and finishing pigs Texto completo
2014
Wagner Azis Garcia de Araújo | Luis Fernando Teixeira Albino | Horacio Santiago Rostagno | Melissa Isabel Hannas | Jorge Armando Prada Luengas | Francisco Carlos de Oliveira Silva | Thony Assis Carvalho | Rosana Cardoso Maia
Aiming to evaluate the performance of pigs from 30 to 100 kg of live a total of 96 pigs with average live weight of 32.19±3.27 kg were distributed in randomized blocks design in a 4 × 2 (four levels of SM: 0, 8, 16 and 24%; with or without inclusion of EC) factorial arrangement with six replicates and two animals per experimental unit. The parameters analyzed were feed intake (kg), weight gain (kg), feed conversion (kg/kg), backfat thickness (mm), carcass muscularity (kg), hot carcass weight (%), percentage of lean meat in the carcass (%), and quantity of lean meat in the carcass (kg). There was no interaction between EC and SM levels in the diet. The addition of EC in the diet affected the performance of the animals, but not carcass characteristics. Feed conversion of animals from 30 to 70 kg weight was improved by the inclusion of EC. The increasing levels of SM in the diet presented quadratic effect on weight gain and on backfat thickness, with inclusion points of SM that promoted maximum values of these parameters of 7.26% and 8.16%, respectively.
Mostrar más [+] Menos [-]Farelo de girassol e suplementação de complexo enzimático em dietas para suínos em crescimento e terminação Texto completo
2014
Albino, Luiz Fernando Teixeira | Araújo, Wagner Azis Garcia de | Rostagno, Horacio Santiago | Hannas, Melissa Isabel | Luengas, Jorge Armando Prada | Silva, Francisco Carlos de Oliveira | Carvalho, Thony Assis | Maia, Rosana Cardoso
Este estudo teve como objetivo avaliar a inclusão de farelo de girassol e a suplementação de complexo enzimático sobre os parâmetros de desempenho e características de carcaça de suínos, dos 30 aos 100 kg de peso vivo. Foram utilizados 96 suínos com peso vivo médio de 32,19±3,27 kg, distribuídos em um delineamento experimental de blocos casualizados, em esquema fatorial 4 x 2 (quatro níveis de farelo de girassol-FG: 0, 8, 16 e 24% com ou sem inclusão do complexo enzimático-CE), com seis repetições e dois animais por unidade experimental. As variáveis analisadas foram o consumo de ração (kg), o ganho de peso (kg), a conversão alimentar (kg/ kg), a espessura de toucinho (mm), a musculosidade (kg), o peso da carcaça quente (%), a porcentagem de carne magra na carcaça (%), a quantidade de carne magra na carcaça (kg). Não houve nenhuma interação entre os fatores para nenhuma das variáveis estudadas. A conversão alimentar dos animais dos 30 aos 70 kg de peso foi diminuída pela inclusão do CE, porém não afetou os parâmetros de carcaça. Níveis crescentes de FG na ração apresentaram efeito quadrático sobre o ganho de peso dos animais e sobre a espessura de toucinho, com valores máximos dessas variáveis em 7,26% e 8,16% de inclusão do FG, respectivamente. | This study aimed to evaluate the inclusion of sunflower meal and an enzyme complex supplement on the performance and carcass parameters in swine from 30 to 100 kg live weight. A total of 96 pigs with average live weight of 32.19 ± 3.27 kg were distributed in a randomized blocks design with a 4 × 2 factorial arrangement of treatments (four levels of sunflower meal-SM: 0, 8, 16 and 24%, with or without inclusion of an enzyme complex-EC), factorial arrangement with six replicates and two animals per experimental unit. The analyzed variables were feed intake (kg), weight gain (kg), feed conversion (kg/kg), backfat thickness (mm), carcass muscularity (kg), hot carcass weight (%), lean meat carcass percentage (%), and lean meat carcass weight (kg). There was no interaction between factors for any of the studied variables. Feed conversion of animals from 30 to 70 kg live weight was improved by the inclusion of EC. This enzyme complex inclusion did not affect carcass characteristics. Increasing levels of SM in the test subject feed diet rations presented a quadratic effect on weight gain and on backfat thickness that reached maximum values in parameters of 7.26% and 8.16%, respectively
Mostrar más [+] Menos [-]Sunflower meal and supplementation of enzyme complex in diets for growing and finishing pigs | Farelo de girassol e suplementação de complexo enzimático em dietas para suínos em crescimento e terminação Texto completo
2014
Wagner Azis Garcia de Araújo | Luis Fernando Teixeira Albino | Horacio Santiago Rostagno | Melissa Isabel Hannas | Jorge Armando Prada Luengas | Francisco Carlos de Oliveira Silva | Thony Assis Carvalho | Rosana Cardoso Maia
Este estudo teve como objetivo avaliar a inclusão de farelo de girassol e a suplementação de complexo enzimático sobre os parâmetros de desempenho e características de carcaça de suínos, dos 30 aos 100 kg de peso vivo. Foram utilizados 96 suínos com peso vivo médio de 32,19±3,27 kg, distribuídos em um delineamento experimental de blocos casualizados, em esquema fatorial 4 x 2 (quatro níveis de farelo de girassol-FG: 0, 8, 16 e 24% com ou sem inclusão do complexo enzimático-CE), com seis repetições e dois animais por unidade experimental. As variáveis analisadas foram o consumo de ração (kg), o ganho de peso (kg), a conversão alimentar (kg/ kg), a espessura de toucinho (mm), a musculosidade (kg), o peso da carcaça quente (%), a porcentagem de carne magra na carcaça (%), a quantidade de carne magra na carcaça (kg). Não houve nenhuma interação entre os fatores para nenhuma das variáveis estudadas. A conversão alimentar dos animais dos 30 aos 70 kg de peso foi diminuída pela inclusão do CE, porém não afetou os parâmetros de carcaça. Níveis crescentes de FG na ração apresentaram efeito quadrático sobre o ganho de peso dos animais e sobre a espessura de toucinho, com valores máximos dessas variáveis em 7,26% e 8,16% de inclusão do FG, respectivamente. | Aiming to evaluate the performance of pigs from 30 to 100 kg of live a total of 96 pigs with average live weight of 32.19±3.27 kg were distributed in randomized blocks design in a 4 × 2 (four levels of SM: 0, 8, 16 and 24%; with or without inclusion of EC) factorial arrangement with six replicates and two animals per experimental unit. The parameters analyzed were feed intake (kg), weight gain (kg), feed conversion (kg/kg), backfat thickness (mm), carcass muscularity (kg), hot carcass weight (%), percentage of lean meat in the carcass (%), and quantity of lean meat in the carcass (kg). There was no interaction between EC and SM levels in the diet. The addition of EC in the diet affected the performance of the animals, but not carcass characteristics. Feed conversion of animals from 30 to 70 kg weight was improved by the inclusion of EC. The increasing levels of SM in the diet presented quadratic effect on weight gain and on backfat thickness, with inclusion points of SM that promoted maximum values of these parameters of 7.26% and 8.16%, respectively.
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