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Establishment of a diagnostic method for porcine proliferative enteropathy using polymerase chain reaction
1999
Lym, S.K. | Lee, H.S. | Woo, S.R. | Yoon, S.S. | Moon, O.K. | Lee, Y.Y. (Ministry of Agriculture & Forestry, Anyang (Korea Republic). National Veterinry Research & Quarantine Service) | Koh, H.B. (National University Kwangju (Korea Republic). College of Betrinary Medicine)
Porcine Proliferative Ebterophthy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 tp 20 weeks of age. PPE has been diagnosed by clinical sighs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which ws a fast, specific and sensitive method for identification of Lawsonia intracellularis(L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonclease analysis with restriction enzyme HaeIII and PstI. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.
Mostrar más [+] Menos [-]Dissemination of Borrelia burgdorferi and immunological responses after experimental infection in rabbits
1999
Kim, J.B. | Park, S.U. | Song, H.W. | Park, S.W. | Kim, Y.M. (Yonsei University, Wonju (Korea Republic). Department of Medical Technology, College of Health Science)
The visceral dissemination of Borrelia burgdorferi in New Zealand White rabbits was evaluated following intradermal inoculation of 1*10 8 spirochetes. We inoculated Borrelia burgdorferi B31, B garinii KW1 and B afzelii S13, respectively, and monitored the dissemination in the experimentally infected rabbits for 28 days. In the B burgdorferi B31-challenged group, the spirochetes were com;oetely cleared in rabbits at day 1 and visceral dissemination was not demonstrated. However, B garinii KW1 and B afzelii S13 were found to successfully disseminate in visceral organs of rabbits during the experiment period of 28 days. And experimentally infection-derived immunological responses in rabbits were identified with enzyme-linked immunosorbent assay and immunoblot analysis. Based on these results, the differences in the virulence of Lymeborrelial strains were proved in rabbit model.
Mostrar más [+] Menos [-]Sex determination of bovine embryos with hamster H-Y antibody and by polymerase chain reaction
1999
Yu, I.J. | Kim, Y.J. (Chonbuk National University, Chonju (Korea Republic). Department of Obstetrics, College of Veterinary Medicine) | Lee, K.K. (Korea Institute of Science and Technology, Taejon (Korea Republic). Korea Research Institute of Bioscience and Biotechnology)
To determine sex of bovine embryos using hamster histocompatibility Y(H-Y) antibodies, bovine compact morulae were incubated for 6 hours in TCM199 supplemented with 10% hamster H-Y antiserum and the embryos with developmental arrest were diagnosed as male embryos, while the embryos showing development during the incubation as femalle embryos. This presumptive embryo sexing was confirmed by polymerase chain reaction(PCR)method. 1. In the result of hamster sperm cytotoxicity test to measure H-Y antibody titer, the rate of dead sperm was considerably lower in H-Y antiserum absorbed with hamster male splenocytes than in H-Y antiserum absorbed with hamster female splenocytes or H-Y antiserum umabsorbed with splenocytes(p0.01). 2. The rate of oocytes fertilized in vitro and the rate of blastocysts of the fertilized oocytes were 58.5% and 32.4%, respectively. The rate of blastocysts on day 8 was 15.9%, denoting the highest rate during whole culture period posterior to in vitro fertilization(IVF). 3. The bovine 16 cell and compact morulae embryos incubated in the medium supplemented with hamster H-Y antibodies showed 37.1% and 48.9% of developmental arrest which were diagnosed as male, respectively, and rates of redeveloped embryos from the arrested were 24.1% in 16 cell and 44.3% in compact morulae embryos, respectively, denoting higher rater of sex determination and rate of redevelopment in compact morulae than 16 cell embryos. 4. Bovine compact morulae of Korean cattle and Holstein were treated with hamster H-Y antibodies for sex determination and the rates fo developmental arrest(deagnosed as male) were 48.4% for Korean cattle and 47.9% for Holstein, respectively. The rates of redeveloped embryos to blastocyst after treatment were 42.6% for Korean cattle and 41.8% for Holstein, respectively, ahowing no sighificant differences of sex determination and redevelopment between both breed. 5. The sex determination of bovine cmbryos(Korean cattle Holstein) using hamster H-Y antibodies was diagnosed by PCR for confirmation, denoting the rates of 86.1% for Korean cattle and 85.9% for Holstein male embryos, respectively, and the rates of 91.9% for Korean cattle and 90.1% for Holstein female embryos, respectively, with no significant differences of sex determination between both breed. These results indicated that hamster H-Y antibodies can be usable for sex determination of bovine embryos of Korean cattle and Holstein, the viability of bovine embryos was sustained while being cultured in the medium supplemented with hamster H-Y antibodies of appropriate titer and sex determination of bovine embryos by PCR can be feasible for confirmation.
Mostrar más [+] Menos [-]Characterization of enzymatic activity of galactose epimerase-less mutant of Salmonella pullorum
1994
Kim, J.B. (Yonsei University, Seoul (Korea Republic). College of Health Science)
Effect of cadmium on immune responses and enzyme activities in BALB/c mice
1997
Yoon, C.Y. (Korean Food and Drug Administration, Seoul (Korea Republic)) | Kim, T.J. | Cho, J.G. | Song, H.J. (Chonbuk National University, Chonju (Korea Republic). Bio-Safety Research Institute)
Indocyanine green excretion test and changes of plasma enzyme activities in dogs
1992
Kim, C.H. (Gyeongsangnamdo Veterinary Service Laboratory, Chinju (Korea Republic). ) | Choi, I.K. | Son, M.S. | Kim, J.G. | Kang, C.B. (Gyeongsang Nat'l Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine)
Studies on catalase type in Cheju native horse
1991
Hyun, H.S. | Kim, W.K. (Chonnam National Univ., Kwangju (Korea Republic). Coll. of Veterinary Medicine) | Chang, D.G. (Cheju Junior Coll., Cheju (Korea Republic))
Effects of administration of Lactobacillus acidophilus-fermented milk on hematological values and histopathological changes of kidney in cadmium-treated rats
1994
Cho, Y.C. (Chungnam National University, Taejon (Korea Republic). College of Medicine) | Jun, M.H. | Chang, K.S. (Chungnam National University, Taejon (Korea Republic). College of Veterinary Medicine)
Activity of myeloperoxidase and leukocyte peroxidase according to ages in the hen (Dekalbwarren)
1994
Chon, S.K. | Kang, C.W. | Lee, H.I. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine)
Indocyanine green excretion test and changes of plasma enzyme activites in Korean native cattle and dairy cattle
1992
Son, M.S. | Choi, I.K. | Kim, J.G. | Hur, J.H. | Kang, C.B. (Gyeongsang Nat'l Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine) | Kim, C.H. (Gyeongsangnamdo Veterinary Service Laboratory, Chinju (Korea Republic). )