A total of 3430 serum samples from various animal species and humans were tested using microscopic agglutination test (MAT) to determine
the frequency of the important leptospiral serovars involved in animals and humans. The sera were screened against 14 serovars of pathogenic Leptospira interogans and 1 serovar of non-pathogenic Leptospira biflexa. Altogether, 441 (12.86%) of the tested serum samples were found to be positive serologically. Tested sera reacted to all 15 serovars used in this study. The most predominant serovar in cattle and sheep is hardjo (39.60% and 66.67%). However, in goat, buffalo and horse, the most frequent serovar detected is hebdomadis (30.00%, 32.58% and 57.14%). In dog, the most predominant serovar is bataviae (19.23%). In humans, the most predominant serovar is cynopteri (3.26%). Among all the samples tested, there were no positive samples from pig and cat. Domestic animals, rodents and
pets can infect the environment or transmit the disease to human or other animals. This study showed that domestic animals could play a role in the epidemiology of leptospirosis and represents a threat to
public health.

Vancomycin-resistant enterococci (VRE) are well-known ascendant nosocomial pathogens. The recent detection of epidemiologic strain
carrying vanA gene in the community of people working with animals and in chickens has brought to the forefront the potential public health danger posed by these organism. The farm environment is a major source of VRE persistence in poultry farms. We carried out survival test to test the survival of the VRE isolates on dry condition and surface test to evaluate the inactivation of the isolates by in-use concentration of commonly used disinfectants. In the survival test, all isolates survived for at least 4 weeks in
colony counts of (1.00 × 103 – 3.86 × 103 CFU/ml) under clean condition and (1.00 × 103 – 2.02 × 104) for soiled condition.
Those that were suspended in 5% BSA solution to mimic organic matter load as obtainable on farms survived for at least 8 weeks at (1.54 × 102 – 1.34 × 103 CFU/ ml). In the surface test, inactivation of VRE isolates by in-use concentration of Lindores®, Omnicide® and Ecos Timsen® was tested using the European surface test (EST). All the tested disinfectants were active against the VRE isolates on both the standard test surface (stainless steel) and our test surface (wooden). The results shows microbiocidal effects (ME) for test disinfectants, i.e. the log10 CFU of micro-organisms compared between test biocide and control treated with distilled water, after 7 min of exposure as follows; Lindores® active on both surfaces 5.24 and 3.17, Ecos Timsen® active significantly on steel 4.90 than wood 2.98 and Omnicide® significantly less active on stainless steel
2.40 than on wood 3.50.