Hepatitis E virus (HEV), norovirus (NoV), and rotavirus (RV) are all hypothesized to infect humans zoonotically via exposure through swine and pork. Our study objectives were to estimate Canadian farm-level prevalence of HEV, NoV [specifically porcine enteric calicivirus (PEC)], and RV in finisher pigs, and to study risk factors for farm level viral detection. Farms were recruited using the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) and FoodNet Canada on-farm sampling platforms. Six pooled groups of fecal samples were collected from participating farms, and a questionnaire capturing farm management and biosecurity practices was completed. Samples were assayed using validated real-time polymerase chain reaction (RT-PCR). We modeled predictors for farm level viral RNA detection using logistic and exact logistic regression. Seventy-two herds were sampled: 51 CIPARS herds (15 sampled twice) and 21 FoodNet Canada herds (one sampled twice). Hepatitis E virus was detected in 30/88 farms [34.1% (95% CI 25.0%, 44.5%)]; PEC in 18 [20.5% (95% CI: 13.4%, 30.0%)], and RV in 6 farms [6.8% (95% CI: 3.2%, 14.1%)]. Farm-level prevalence of viruses varied with province and sampling platform. Requiring shower-in and providing boots for visitors were significant predictors (P < 0.05) in single fixed effect mixed logistic regression analysis for detection of HEV and PEC, respectively. In contrast, all RV positive farms provided boots and coveralls, and 5 of 6 farms required shower-in. We hypothesized that these biosecurity measures delayed the mean age of RV infection, resulting in an association with RV detection in finishers. Obtaining feeder pigs from multiple sources was consistently associated with greater odds of detecting each virus.

This study was conducted to investigate the high mortality of young lambs in two sheep farms in Pekan, Pahang over a period of 3 years. Samples from postmortem of 1,451 lambs below one year of age by a farm veterinarian were submitted for laboratory diagnosis at the Bacteriology Section of the Regional Veterinary Diagnostic Laboratory in Kuantan. Escherichia coli is the most commonly recorded bacteria with 161 lambs diagnosed in 2013. In 2014 and 2015, there was a decrease in occurrence of E. coli related deaths, with 120 and 75lambs respectively. A total of 25% of the cases showed Escherichia coli positive by culture on blood agar and MacConkey agar, and confirmed by biochemical tests. A total of 21% of the cases were positive for staphylococcus sp, 3% and 6% for Streptococcus sp and Klebsiella pneumonia, respectively. Other bacteria were isolated in 45% of the cases. It was further noted that a total of 285 lambs between the ages of one to four months of age followed by 58 lambs (20%) less than one month old had E.coli isolation. It is also noteworthy that there were 10 lambs with E.coli infection in one to fourteen day-old lambs during the 3-year period from January 2013 to December 2015. This information was collated as a result of routine diagnosis of field cases submitted and with the intention of highlighting the common pathogens causing high mortality in local small ruminant farms so that preventive action may be taken for future farming ventures. E. coli infections or Colibacillosis is an important finding and indicator of poor management including poor nutrition, hygiene and environmental contamination which can reduce animal immunity and render it susceptible to other infections.

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA) a contagious and infectious disease in smallruminants. This report describes a case of CLA in a Boer cross goat infected with Corynebacterium pseudotuberculosis. The manifestation of abscess in the superficial lymph node presented as a mass ofapproximately 5 cm by 5 cm in diameter at the base of the ear. The mass was solid and non-movable upon palpation. The mass was incised and the caseous material was aspirated and sent to the bacteriologylaboratory where Corynebacterium pseudotuberculosis was isolated. Thewound was cleaned and flushed with diluted chlorhexidine and iodine solutions daily. This procedure aids in the complete healing of the wound after a few weeks. However, the agent of the disease persistsin the animal in its entire life and culling is recommended to prevent the spread of the disease in the herd.

In the Malaysian environment horses are primarily used in sports activities such as racing, endurance, dressage and show jumping as well as in recreational pursuits and police work. Recently, the Perak Turf Club witnessed the death of a four-yearold thoroughbred mare which was given enrofloxacin injection as treatment and was regularly dewormed and vaccinated againstequine influenza, Japanese encephalitis and tetanus. Post-mortem examination of intestinal contents revealed presence of worms. The sample was then sent to the Veterinary Research Institute (VRI), Perak for morphological identification of the worm. The worm was identified as Parascaris equorum. Thus, awareness ongastrointestinal parasites should be raised especially by recommending improved management practices such as proper manure disposal and deworming procedures to control parasite infestations as well as good management and nutrition.

In Malaysia, helminthiasis due to strongyles such as Haemonchuscontortus and coccidiosis caused by Eimeria sp. have been reported to cause severe economic losses in small ruminants livestock industry. This paper reports the occurrence of gastrointestinal parasite infections on small ruminants situated in Seberang Perai Selatan district, Penang. Faecal samples were obtained from a total of 193 animals,randomly selected from 14 ruminant farms. The results of this survey indicates that helminthiasis and coccidiosis is rampant insheep and goat farms. The most common infections diagnosed were helminthiasis (77.72%) and coccidiosis (60.10%) followedby Moniezia sp. (5.18%). From this study, it shows that parasitic diseases can be managed by good animal husbandryin farms since high parasitic infections were observed in farms that were poorly managed based on nutrition, hygiene andbasic animal husbandry practices. The smallholders depended on health and extension services from the State Veterinary Department. A continuous monitoring of small ruminant farms by the Department of Veterinary Services will provide important information for assisting farmers with managing the spread of parasitic infections and maintaining the productivity of animals.

Salmonella is an important cause of foodborne illnesses in humans. Food-producing animals, including swine, are a major source of Salmonella in food products. This study investigated on farm Salmonella fecal shedding in pigs at different production stages - from weaning to marketing - and its association with the presence of Salmonella in tissues at slaughter. Fourteen groups from 8 commercial farrowing sources (N = 809 pigs) were monitored 5 times from birth to slaughter. Fecal and tissue samples were collected from pigs and cultured for Salmonella. A survey was conducted to collect farm management information. A multi-level mixed-effects logistic regression modelling method was used to analyze Salmonella shedding over time and the association between Salmonella shedding and the presence of Salmonella in tissue samples. Salmonella was recovered from 13% (421/3339) of fecal samples collected from 809 pigs over the course of the study. Overall, 35% (284) of pigs shed Salmonella at least once, while 12% (99) shed more than once. Salmonella shedding increased as pigs aged (P = 0.01) and increased in the summer months (P < 0.01). Salmonella was isolated from tissue samples collected from 23% (134/580) of pigs; however, the presence of Salmonella at slaughter was not associated with on farm shedding. The seasonal trend in Salmonella shedding and its association with age may be used to identify high-risk groups and implement more effective control measures accordingly. The identification of repeat shedders warrants interventions that target this source of infection on swine farms.

In most countries, poultry are reared by traditional farmers d ue tothe relative minimum capital needed to start off, availability of feed and the fast period of the birds to grow. This research was conducted on turkeys which aims to study the abundance and prevalence of ectoparasites from three localities around Kedah, Malaysia. Atotal of 20 turkeys (eight males and twelve females) were examined for ectoparasites infestation and endoparasites infection.Six species of ectoparasites: five lice and a mite have been discovered. The most prevalent ectoparasite was Menopon gallinae with occurrence of 45%. Other external parasites recorded includeLipeurus caponis and Megninia cubitalis with occurrence of 40%, Menacanthus pallidulus (35%), Goniocotes gallinae (30%) and Chelopistes meleagridis (20%). There was a significant difference between the ectoparasite abundance in Jabatan Perkhidmatan Veterinar and Alor Belat Barat [ANOVA, F(2,17)=6.33, p=0.009]. These lice commonly found in the fluff of the feathers, especially at the neck,abdomen, and wings. Lipeurus caponis was found to have the highest infestation in all male and female turkeys and Menacanthus pallidulus was noted as the less common ectoparasite infesting both the male andfemale turkeys. Endoparasite infection was recorded in two species of parasite eggs of nematodes and protozoa. Oocyst of Eimeria spp. recorded the highest faecal egg count with 7300 epg compared to Capillaria spp. with only 1200 epg. Scavenging and pecking behaviour of turkeys in barn area with unsuitable farm housing environmentwere the major contributing factors to the infestation of ectoparasites as well as endoparasites infection.

This study was conductedon raw cow’s milk collected in seven statesof Malaysia to investigate the compositionof minerals and trace elements in the milk.Raw cow’s milk samples were collectedfrom Kedah, Perak, Johor, Melaka,Pahang, Selangor and Negeri Sembilanfrom January to November 2015. ICPMStechnique combined with microwavedigestion were applied to determine theconcentration of five major minerals (Na,Mg, K, Ca and P) and eleven trace elements(Cr, Mn, Fe, Ni, Cu, Zn, Se, Sr, Mo,Baand Pb) in the milk samples. The resultof analysis showed relatively constantcomposition of minerals and trace elementsin the samples but some variations wereobserved in certain states. Application ofprincipal component analysis (PCA) in thecomposition data showed different clusterexists between the states of Selangor andNegeri Sembilan as well as Kedah andNegeri Sembilan. There is no significantdifference observed in the compositionsof minerals and trace elements betweenother states. The compositions of mineralsand trace elements in raw cow’s milk inMalaysia are comparable with reports fromother research. Further studies can be doneto investigate the source of nutrient, foodor environmental condition that producedthe amounts of minerals and trace elementsin the milk.

A total of 2803 bloodsamples from ruminant livestock werereceived for diagnosis for parasiticinfections from 2011 to 2015. Theruminant samples were from small holderand commercial farms from the states ofNegeri Sembilan, Melaka, Selangor andthe Federal Territory. The results indicatethe presence of theileriosis, anaplasmosis,babesiosis and trypanosomiasis in theseanimals. The most common parasiteidentified annually is theileriosis in cattle.This information is vital for disease controlstrategies as most blood protozoa causemorbidity and is transmitted by vectorssuch as ticks.

Trypanosomiasis positive cases were reported in Sahom Farm Retreatin Gopeng, Perak; with multispecies livestock animals. Nzi and Vavoua traps were applied to survey the population of biting flies; stable flies (Muscidae: Stomoxyinae) and horse flies (Tabanidae)as the vector for surra. Results indicated the presence of Trypanosomiasis infection diagnosed by buffy coat examination, thinblood stained smears and serological test (Surra Sero K-Set test) and identification of its insect vectors. The presence of bothbiting flies provides the missing link between the occurrence of the disease and host or environmental factors precipitatingthe disease. Besides trypanosomiasis in cattle, other parasitic infections were also recorded with heavy infections for liver fluke (Fasciola gigantica ova) and coccidia oocysts. Therefore, some control measures are recommended to eradicate the vectors and to treat infected animals in order to prevent the dissemination ofthe trypanosmiasis.