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Enzyme-linked immunosorbent assay for screening of milk samples for Salmonella typhimurium in dairy herds.
1995
Hoorfar J. | Wedderkopp A.
We investigated the ability of an antibody-specific, O antigen-based ELISA to document Salmonella typhimurium herd infections by screening of milk samples. Three cattle populations, 20 herds with no history of salmonellosis, 8 herds with history of S. typhimurium episodes within the previous 7 months, and 220 herds of unknown disease status, were tested. A herd was considered ELISA positive if at least 5% of the cows had OD values > 0.3. Among the 20 herds without history of salmonellosis, only 2 herds were ELISA positive, whereas all 8 herds with a known history of salmonellosis were ELISA positive (herd specificity, 0.9 and herd sensitivity, 1.0). A significant correlation (P < 0.001) was found between the OD values of serum and milk samples from cows in the herds with a history of salmonellosis. It was concluded that ELISA testing of individual milk samples can be used for surveillance of herds for S. typhimurium infections, but further modifications are needed to test bulk tank milk samples.
Mostrar más [+] Menos [-]Causes of calf mortality in Kabete area of Kenya
1995
Mulei, C.M. (Nairobi Univ. (Kenya). Dept. of Clinical Studies) | Gitau, G.K. | Mbuthia, P.G.
The use of preserved colostrum for rearing replacement dairy calves: calf performance, economics and on-farm practicability in Kenya
1995
Karioki, D.I. (Nairobi Univ. (Kenya). Dept. of Clinical Studies) | Gitau, G.K. | Munyua, S.J.M.
