A total of 180 eggs (20 batches each represented by 3 eggs) of the farm, balady, and organic egg samples were collected in October 2021 from markets in Ismailia governorate, Egypt, and examined for detection of the oxytetracycline, ciprofloxacin, and gentamicin residues by using HPLC-Ms/Ms. The farm and balady eggs contained 20% with mean values of 69.95±1.78 and 43.6±2.65 ppb, respectively, and all of them were below the permissible limits and accepted. They contained 20% and 60% with mean values of 26± 0.21 and 39.58±5.58 ppb, respectively, and all positive samples were unaccepted. Both egg types were free from gentamicin residues, meanwhile, all organic eggs were clear from the three antibiotic residues under investigation. The study also revealed the distribution of oxytetracycline and ciprofloxacin in albumin and yolk after oral administration and their residues in the 6th-day eggs were (750 and 500 ppb) and (500 and 319 ppb), respectively. The effect of boiling for 15 minutes on oxytetracycline and ciprofloxacin residues in albumin and yolk was also investigated and showed a reduction% of (88 and 90) and (90.5 and 87.5), respectively. Meanwhile, the frying of whole eggs for 5 minutes reduced their concentration by 94.3% and 94.5%, respectively. In conclusion, the antibiotic residues in edible eggs are a serious risk that should be monitored, and it's significant to highlight the importance of efficient boiling and frying of eggs in reducing these residues and relieving their hazards to the consumer's health.

The purpose of this investigation was to ascertain if orally administered Moringa oleifera (MO) extract had any protective effects on several biochemical markers in the kidney and liver in gentamicin (GNT)-induced hepato-renal toxicity in rats. Forty male albino rats were divided into four groups: the control group, the MO treated group, the GNT administered group, and the (MO+ GNT) group. The MO+ GNT group received GNT (100 mg/kg b.wt, i.p.) together with Moringa oleifera (400 mg/kg b.wt) for 20 consecutive days. Rats were put to death at the conclusion of the experiment, and blood samples were taken to measure serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total proteins, albumin, and globulin, as well as serum urea, creatinine, and uric acid. Catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (TAC), 8-hydroxy-2' -deoxyguanosine (8OHdG), and malondialdehyde (MDA) were measured in isolated kidneys and liver. The liver and kidneys were divided into pieces for histology and a few immunohistochemistry tests. Following administration of GNT, there was a significant decrease in the activities of the hepatic and renal CAT, GPX, GSH, SOD, and TAC while there was a significant increase in the levels of MDA, 8OHdG, serum AST, ALT, ALP, urea, creatinine, and uric acid. Treatment with MO significantly lessened the histopathological abrasions in the liver and kidney tissue brought on by GNT and restored the levels of renal and hepatic BAX and TNF. It also restored the evaluated criteria to normal values. According to the results, MO has a protective effect against GNT-induced hepato-nephrotoxicity in rats. This effect may be explained by the fact that MO prevents free radical generation and restores antioxidant activity, which reduces the negative effects of GNT.

Gentamicin (GEN) is considered an aminoglycoside antibiotic which is widely used to treat numerous bacterial infections. It has toxic effect on liver tissue. Curcumin (CMN) is a natural polyphenolic compound with antioxidant as well as anti-inflammatory potentials. The current study sought to investigate the effect of CMN in protecting against GEN-induced hematological alterations and hepatotoxicity. Rats were randomly assigned into 4 equal groups: Control, gentamicin group (100 mg/kg b.wt, i.p, daily for seven days), curcumin group (200 mg/kg b.wt, orally for 21 days) and curcumin plus gentamicin group. After 21 days, some hematological and serum biochemical parameters were measured. GEN-intoxicated group showed a marked decline in RBCs count, Hb concentration as well as PCV% with insignificant difference in MCV, MCH and MCHC. Moreover, there were leukopenia, lymphopenia and neutrophilia in GEN group. Concerning to the serum biochemical examination, a substantial increase in the serum activities of ALT, AST and ALP with a marked decline in the total protein, albumin and globulin serum levels were recorded following GEN injection. In addition, there was a marked increase in the cholesterol, triglycerides and glucose serum levels. Improvement in all tested parameters were noticed following concurrent CMN administration with GEN. Based on these results, CMN could be recommended as a treatment strategy for protection against GEN-induced hematological alterations and hepatic injury.