Effects of dietary aflatoxin (AF) and supplemental vitamin E (d-alpha-tocopherol) were evaluated in growing crossbred pigs. Nine barrows (3 replicates of 3 each, mean body weight, 14.0 kg) per group were assigned to 1 of 4 treatment groups (for a total of 36 barrows): 0 IU of supplemental vitamin E and 0 mg of AF/kg of feed (control); 2,400 IU of vitamin E divided into equal doses and administered IM on days 1 and 16; 2.5 mg of AF/kg of feed; or 2.5 mg of AF/kg of feed plus 2,400 IU of vitamin E administered similarly to treatment 2. Barrows were administered their respective treatment for 32 days. Evaluations were made for group production performance and for serum biochemical, immunologic, hematologic, pathologic, serum and tissue tocopherol, and serum retinol variables. Body weight was reduced by AF-alone and AF plus vitamin E treatments, compared with control and vitamin E-alone treatments. Liver weight was increased for the AF alone-treated and the AF plus vitamin E-treated barrows, compared with control barrows. The AF alone-treated barrows had alterations in: serum values of alkaline phosphatase, gamma-glutamyltransferase, albumin, glucose, phosphorus, calcium, cholesterol, total iron, unsaturated iron-binding capacity, total iron-binding capacity, and urea nitrogen; RBC numbers, hematocrit, hemoglobin concentration, and prothrombin time; and mitogen-induced lymphoblastogenic responses. With the exception of some slight ameliorating effects on hematologic measurements, supplemental treatment with vitamin E did not prove beneficial against the toxicosis-associated AF treatment. The AF alone-treated barrows had decreased serum tocopherol and retinol concentrations, compared with control and pretest values, and decreased tocopherol concentration in cardiac tissue. High parenterally administered doses of vitamin E did not have sparing effect on Af-induced reductions of serum tocopherol or retinol concentration; however, compared with pretest values, serum tocopherol concentration was increased by vitamin E-alone treatment. Tocopherol concentration in cardiac tissue of the AF plus vitamin E-treated barrows was increased over that of the AF alone-treated barrows, indicating an ameliorating effect on AF-induced tissue concentrations reductions. These data indicate that vitamin E may not have a sparing effect on AF-induced toxicosis and that AF may reduce serum retinol and serum and tissue tocopherol concentrations.

Beagles were exposed to aerosols of (239)PuO2, (238)PuO2, or (239)Pu(NO3)4. Exponential growth constants for 50 primary lung tumors (23 bronchioloalveolar carcinomas, 22 papillary adenocarcinomas, 5 adenosquamous carcinomas) were calculated in 37 dogs, using sequential thoracic radiography. A wide range in doubling time (6 to 287 days) was observed. Mean +/- SEM doubling time was 93 +/- 10 days for bronchioloalveolar carcinoma, 107 +/- 13 days for papillary adenocarcinoma, and 101 +/- 36 days for adenosquamous carcinoma. Lung tumor growth rate in dogs was comparable to that in human patients with similar histologic tumor types. Linear regression analysis revealed significant (P less than or equal to 0.0001) correlation between doubling time and survival of individual dogs. Doubling time was not significantly dependent on tumor type, sex, age at time of diagnosis, initial lung deposition, or isotope. Extrapolating time to tumor onset from tumor doubling time cannot be used to reliably predict the onset of malignancy.

A primary fibroblast cells from embryos of brown quail Coturnixypsilophora has been established and partially characterized. The cells were maintained in Modified Eagle’s medium (MEM) supplemented with 10% fetal bovine serum. The cells were able to grow at temperatures between 35°C and 38°C with optimum temperature of 37°C. The growth rate of primary quail fibroblast cells increased as the FBS proportion increased from 5% to 20% at 37°C with optimum growth at the concentrations of 10% or 15% FBS. The cells showed no microbial contamination throughout the period of experiment and the total chromosome number of a diploid cell was 78, according to karyotyping and chromosome analysis. The susceptibility of quail primary cells for avian viruses was investigated in this study after inoculation with ND and IB viruses. Both viruses showed a satisfactory CPE development and the infectivity were assayed by virus titration (TCID50). This suggests that the quail primary cells can be used for isolation of various avian viruses with further steps of infectivity confirmation in the future.

Kedah-Kelantan cattle (KK) being an indigenous breed are highly adapted to the hot-humid Malaysian climate and can survive in harsh, marginal environments. This makes the KK a valuable genetic resource, given the challenges of climate change and the changing demands of the livestock sector. Hitherto there is no comprehensive programme to genetically improve the purebred KK. Genetic improvement of the KK would be to fulfill the breeding objectives of increasing lean meat growth rate, enhancing meat quality, raising feed efficiency, improving fertility and maintaining adaptability. The breeding structure proposed is a 2-tier breeding structure, with a nucleus tier followed by a commercial tier below it. The nucleus tier would comprise of a number of pedigree farms run as a community
breeding project. A sire reference scheme is proposed, where progeny of reference sires are used as genetic links between pedigree herds and between years. Some guidelines are offered on the establishment
and implementation of the scheme. Modern breeding technology such as BLUP using an Animal Model, artificial insemination, embryo transfer, tissue scanning, MAS and MAI could be used as tools to support the KK genetic improvement programme. To address the problem of genetic erosion, emphasis should be given to the conservation and sustainable utilization of the KK. The programme is expected to have a high impact on the livestock sector. Substantial investments are needed to develop infrastructure and human capital associated with the KK breeding programme. The establishment of a KK breed society will improve awareness concerning the KK and protect the interests
of the KK breeding community.

Growth dynamics of buffalo population and milk production and productivity in Tamil Nadu was analysed. Point to point annual compound growth rate (ACGR) was calculated to assess the trend in buffalo population and continuous annual compound growth rate (ACGR) for milk production and productivity. The share of Tamil Nadu to National buffalo population decreased from 5.29 to 0.72 % (1951 to 2012) and the buffalo milk production reduced from 4.01 to 0.46% (2001-02 to 2015-16). The ACGR of buffalo population in Tamil Nadu was -17.23% during the last inter-census period (2007-12). North Eastern and Cauvery Delta agro-climatic zones of Tamil Nadu registered high negative growth. The annual compound growth rate of buffalo milk production during 2011-12 to 2015-16 in  Southern zone was -27.37% and it was - 21.72% in North Eastern and -20.93 % in Cauvery Delta zone with overall ACGR of -16.85%. The ACGR of buffalo milk productivity was 0.61% (2001-02 to 2015-16) in Tamil Nadu, while the rate was 1.58% in India. Hence, rigorous implementation of buffalo development programmes is a prime requisite and need of the hour for improving buffalo population and milk production in Tamil Nadu.

The growth and development of replacement gilts of different precocity and age depending on the terms of transition from herd-producer to the market area of a complex was studied in the conditions of the Republic of Belarus. Animal transfer into new housing conditions affected the early maturing and super early maturing breed pigs of experimental groups. It was determined that moving of replacement pigs into the market area of the complex at the age of 115 days made it possible to obtain higher indices of swine productivity. Fertilization rate of sows of the first experimental group after the first insemination made up 77,6%, that was higher on 6,2% and 9,5% in comparison with the control and the second experimental groups, respectively. The advantage of the first and second experimental groups over the control one was stated in a higher multiple fetation on 0,4 heads and heightened heavy litter indices on 0,1 kg; the quantity of weanling was higher on 0,6 and 0,5 heads, on litter weight at weaning was higher on 7,1 and 3,8 kg, respectively

In the conditions of the Republic of Belarus there was realized an ecological monitoring of sources of water supply in the conditions of a swine breeding complex and adjoining settlements. There was studied the influence of a large swine breeding complex on water quality of closed type water sources. There was marked a microbial contamination of water depending on remoteness of animal breeding object and season of a year. There were determined the seasonal changes of physical properties, chemical and bacteriological composition of water used for swine breeding. There were noted changes in water quality in the process of its flow from the point of water inlet up to the place of its consumption. Results of influence of improved water on piglets were presented. There was studied the possibility of improvement of water quality by means of its treatment with solution of potassium hypermanganate (0,01%) which made it possible to increase average daily live weight gain of piglets. In course of the study there was realized the analysis of protein spectrum of blood serum of piglets; dynamics of body weight and average daily weight gain of experimental piglets.