The sensitivity of an indirect enzyme-linked immunosorbent assay (ELISA) for bovine IgG serum antibody to Pasteurella haemolytica was compared with that of an indirect hemagglutination (IHA) test. Pasteurella haemolytica serotypes were grown in a chemically defined cell culture medium, and soluble antigens released into the growth medium were used in the ELISA and IHA test. An ELISA with serotype-1 antigen consistently detected antibody in sera that were positive by IHA test (correlation, 99%). Sera reacting with serotype-1 ELISA antigens also reacted with ELISA antigens prepared from other serotypes. Although ELISA titers determined by the 2 methods were approximately linear. Titer increases detected in paired serum samples by either test were similar. The ELISA was more sensitive than was the IHA in detecting colostral IgG antibody in serum of newborn calves. The ELISA uses a simple, stable antigen preparation and detects antibody to P haemolytica serotypes that commonly infect cattle.

The ability of Mycoplasma hyopneumoniae to agglutinate RBC was evaluated to develop an in vitro cytadsorption assay. Using swine RBC in a microtitration hemagglutination test, no agglutination or partial agglutination was detected. Comparison of RBC from various other species indicated that improved hemagglutination was obtained with RBC from turkeys. This hemagglutination was detected only when mycoplasma cells used in the assay had been frozen and thawed, heated at 50 C for 30 minutes, or treated with trypsin. Treatment of RBC with trypsin or neuraminidase enhanced hemagglutination. Possible surface lectin activity in M hyopneumoniae was evaluated by use of carbohydrates in a blocking assay; hemagglutination was not inhibited by any of 13 carbohydrates evaluated. Mycoplasma hyopneumoniae convalescent porcine serum and monoclonal antibodies against 2 M hyopneumoniae immunogens of molecular weights of 64,000 and 41,000 inhibited hemagglutination.

An ELISA was developed and tested to detect antibodies to Eperythrozoon suis in swine. Results were compare with those of the indirect hemagglutination (IHA) test. Antigen isolated from swine heavily infected with E suis was used for both tests. Comparison of the ELISA with the IHA test revealed a significant (P < 0.001) correlation between results. Of 114 samples obtained from 9 swine infected with E suis, 8 7.7% were seropositive (titer greater than or equal to 200) via the ELISA, and 80.7% were seropositive (titer greater than or equal to 20) via the IHA test. The sensitivity of the ELISA was greater than that of the IHA test. All blood samples obtained from specific-pathogen-free swine tested negative for E suis antibody. Cross-reactions were not observed between E suis antigen and antisera against various swine and cattle disease agents using ELISA. We concluded that the ELISA may be used for rapid and effective diagnosis of infection with E suis in swine.

A total of 128 faecal samples of dogs suspected for canine parvovirus infection were examined by Haemagglutination (HA) and Haemagglutination inhibition (HI) tests. The prevalence of canine parvovirus infection was 45.30% (58/128) and the age wise maximum (70.20%) prevalence was found in dogs upto 6 month. The breed wise maximum (56.90%) prevalence was in Non-descript dogs and sex wise prevalence revealed no significant difference in male (45.9%) and female (43.3%).

A mixed viral infection of Newcastle Disease (ND), Marek’s Disease (MD) and Avian Leukosis (AL) was reported in village chickens. Analysis of the deduced amino acid sequences of the F protein cleavage site of ND virus showed that the isolate was virulent with sequence KRRKR for the C-terminus of the F2 protein and phenylanine (F) at residue 117, the N-terminus of the F1 protein. Basic Local Alignment Search Tool (BLAST) analysis showed the isolate was ND which was 96% similar identity with Indonesia Sukorejo genotype VII, MD 99% similar identity with China very virulent strain and ALV 100% similar identity with Taiwan ALV strain. Due to the free ranging type of management, causes of the diseases in the poultry were uncertain as many factors can contribute to the disease occurrence. However, good hygiene practices can help to improve the farm’s sanitary. Basic biosecurity system can be applied although the success may be limited. Vaccination is another alternative that can be considered to prevent the diseases with the help from certain parties.

The hemagglutinin (HA) of six H5 influenza virus strains isolated from ducks in Japan and China in 1976 to 1996 were analyzed antigenically and genetically. Antigenic analysis using a panel of monoclonal antibodies revealed that the HA of H5 influenza viruses isolated from ducks are antigenically closely related to each other. Phylogenetic analysis indicates that the isolates from ducks in Hokkaido were derived from an ancestor common with the highly pathogenic isolates from chickens and human