Cancerous tumors are one of the main problems that cause of deaths in pet animals. The objective of this paper is to describe histopathological features of some neoplastic masses with confirmation using immunohistochemistry in pet animals.  Our study was done from May 2022 to August 2023 in Sharkia governorate, Egypt to correctly recognize 7 cases of pet animal neoplasms "5 cases of dogs and 2 queen case" based on histopathological and immunohistochemical findings.  The results revealed 2 cases of TVT in Penis with positive expressions for Iba2 and vimentin  , 1 Case of Sebaceous adenoma in Hock joint of male dog skin, 1 case of hepatoid carcinoma in perianal glands of male dog with positive immunolabelling for CK7  , 1 case of chondroblastic osteosarcoma  in A 4-year old, male, Great Dane dog  and 2 cases of queen with mammary gland adenocarcinoma with positive immunoreactivity for HER-2 and other mixed mammary neoplasm accompanied with sarcoma has positive staining for vimentin. It could be concluded that  the histopathological evaluations by H&E stain is the backbone method for neoplasms diagnosis and  using some  biomarkers added a confirmatory tools for these diagnosis.

Climate change has caused extensive changes to marine ecosystems in recent decades; this might lead to an increase in marine disease. Parasite infestation in silver grunt and black finned sea bream is still few. This study aimed to determine the prevalence and intensity of intestinal helminthes of 60 sample of Silver grunt and 60 samples of black finned sea bream species collected from Kuwait fish markets. The result revealed that the total infection rate in each species was 58.3% and 33.3% respectively.  The endoparasite worms found in the intestine of Silver grunt were Procamallanus sinespinis which recorded the highest infestation rate 26.6% with intensity 2, followed by Aephnidiogenes senegalensis (25%) followed by Prosorhynchus indicus (18.3%) with intensity 4 for each, Anisakis physeteris and Aniskais simplex with infestation rate 11.6, 8.3%, respectively and intensity of 2 and 4, respectively. In addition, the endoparasite worms found in the intestine of black finned sea bream was Hysterothylacium fabri (8.3%). Histopathological examination of infested intestine showed necrosis, degenerative changes, and congestion of intestinal blood vessels.

Ivermectin is a broad-spectrum antiparasitic drug with outstanding efficacy and a wide margin of safety. This drug has been widely utilized in veterinary medicine since 1987, and its use in humans has grown. Ivermectin is a broad-spectrum anti-parasitic agent licensed by the FDA. Ivermectin is a mixture of 80% or more avermectin B1a (AB1a) analogue and 20% or less avermectin B1b analogue. Iverzine has been proven to have widespread antiviral action in recent years. Ivermectin has also a broad-spectrum activity against the viruses that cause dengue, Zika, HIV, and yellow fever in vitro. Despite this in vitro action, no clinical trials have shown that ivermectin has a therapeutic benefit in patients with these viruses. Some ivermectin studies have also revealed potential anti-inflammatory qualities, which may be beneficial in persons with COVID-19. However, the use of ivermectin in animals was also associated with some histopathological effects in the internal organs including the liver, kidney, spleen, and brain. The purpose of this review was to provide light on the mechanism of action of ivermectin and its related histopathological consequences in various animal species.

Escherichia coli (E.coli) infections pose significant health risks to poultry and humans globally. In the face of antibiotic-resistant strains, metal oxide nanoparticles and plant extracts have emerged as promising alternatives. This study evaluated the effectiveness of copper oxide nanoparticles (CuO-NPs) and black elderberry extract (BEE) against E. coli O78 infection in specific pathogen-free chicks. The birds were divided into four groups: a negative control NC, a positive control (PC; challenged with E. coli O78), CuO-NPs treatment post-challenge, and BEE treatment post-challenge. Various parameters, including clinical signs, organ lesions, bacterial load, biochemical markers, and histopathology, were assessed. Both CuO-NPs and BEE demonstrated a reduction in clinical morbidity, organ damage, and E. coli counts compared to the PC. However, BEE exhibited slightly greater efficacy in alleviating liver and kidney dysfunction. Overall, CuO-NPs and BEE hold therapeutic potential against avian colibacillosis, likely acting through antimicrobial and immunomodulatory mechanisms. These findings underscore the potential of bio-based nanoparticles and plant extracts as safer, multi-modal alternatives to antibiotics for avian colibacillosis. Further optimization and mechanistic studies are needed to support their clinical application.

Chronic cadmium exposure is known to be a major health concern due to its nephrotoxic effects. Consequently, this study aimed to assess the potential protective effects of date palm fruit extract (DPFE) against cadmium-induced renal toxicity in male albino rats. A total of 48 rats were divided equally into four groups and subjected to specific treatments. Group I (control given 1 ml distal water orally daily), group II (200 mg DPFE /kg b.wt. orally daily), Group III (5 mg CdCl2/kg b.wt. orally, twice a week), and VI (DPFE and CdCl2). Serum samples were collected after 4 and 8 weeks for biochemical analysis, and kidney tissue specimens were obtained for histopathological examination. The administration of cadmium chloride for 4 and 8 weeks resulted in a significant (p<0.05) increase in the serum creatinine, urea, and uric acid levels, indicating kidney dysfunction. Histopathological changes, including vascular lesions, hemorrhage, edema, periglomerular, perivascular and interstitial fibrosis, mononuclear leucocytic cellular infiltration and excessive glomerular and tubular degeneration and necrosis which further confirmed the nephrotoxic effects of cadmium. However, pre-treatment with DPFE exhibited a substantial ameliorative effect against cadmium-induced nephrotoxicity. DPFE supplementation led to a significant reduction in kidney biochemical parameters toward normal levels and improved renal tissue architecture. Our findings indicate that date palm fruit extract has a time-dependant protective effect on kidney function indices and histopathological alterations induced by cadmium, highlighting its potential as a natural medication to mitigate renal damage caused by chronic cadmium exposure.

Objective: The experiment aimed to determine the effects of lipopolysaccharide (LPS), polymyxin B, and honey on survival rates, hematological parameters, liver and kidney biomarkers, blood glucose levels, serum insulin levels, and histopathology of the liver, kidney, lungs, brain, and pancreas in LPS-challenged mice. Materials and Methods: 50 male Swiss Albino mice (Mus musculus), aged 3 weeks, were randomly assigned into 5 groups (10 mice per group): Control group (A), LPS (2 mg/kg bwt/day IP in NS) treated group (B), polymyxin B (1.2 mg/kg bwt/day IM) pre-treated plus LPS (2 mg/kg bwt/day IP in NS) treated group (C), honey (10 gm/kg bwt/day PO) pre-treated plus LPS (2 mg/kg bwt/day IP in NS) treated group (D), both polymyxin B (1.2 mg/kg bwt/day IM) and honey (10 gm/kg bwt/day PO) pre-treated plus LPS (2 mg/kg bwt/day IP in NS) treated group (E). The LPS was administered intraperitoneally (IP) at 80 μg/mice/day, diluting in normal saline. After 16 weeks, the mice were sacrificed, and blood samples and organs (liver, kidney, lung, brain, and pancreas) were collected for laboratory tests. Results: The results revealed that in LPS-treated mice, the mortality rate was the highest, and hemato-biochemical parameters were altered. Histopathological examination in the group treated with LPS showed disarrangement of hepatocytes, cellular infiltrations in the glomerulus, alveolar congestion in the lungs, several nerve fiber degenerations in the brain, and degenerative changes in pancreatic islets. The mortality rate and hemato-biochemical and histopathological changes were restored by the combined treatment of polymyxin B and honey. Conclusion: LPS has detrimental effects on survival rate and hemato-biochemistry, which are lessened by taking honey and polymyxin B supplements. [J Adv Vet Anim Res 2024; 11(2.000): 503-515]