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Divergent diagnosis from arthroscopic findings and identification of CPII and C2C for detection of cartilage degradation in horses
2010
Lettry, V., Hokkaido Univ., Sapporo (Japan) | Sumie, Y. | Mitsuda, K. | Tagami, M. | Hosoya, K. | Takagi, S. | Okumura, M.
The objective of this study was to investigate the changes in synovial fluid concentration of collagen type II cleavage site (C2C) and pro collagen II C-propeptide (CPII), markers of joint cartilage degeneration and synthesis, respectively, in horses with intraarticular fracture or osteochondrosis dissecans (OCD), and to examine the relationship between arthroscopic findings and these biomarker levels. Synovial fluid was collected from 36 joints in 18 horses (6 fractures and 12 OCDs). Samples from contralateral normal joints, when available, served as controls (n=12). Concentrations of C2C and CPII were measured using enzyme-linked immunosorbant assays. Moreover, the severity of the cartilage degradation was graded arthroscopically in 16 horses, and the correlation between the C2C and CPII levels and the arthroscopic scores were investigated. Compared to the control, the concentration of C2C was increased in OCD joints but not in fracture joints, whereas the concentration of CPII was increased in fracture joints but not in OCD joints. Within each disease group there was no correlation between biomarker levels and arthroscopic findings. Therefore, although C2C and CPII have diagnostic potential further knowledge is required to provide accurate analysis.
Mostrar más [+] Menos [-]Distribution of TNF receptors and TNF receptor-associated intracellular signaling factors on equine tendinocytes in vitro
2004
Hosaka, Y. (Rakuno Gakuen Univ., Ebetsu, Hokkaido (Japan)) | Sakamoto, Y. | Kirisawa, R. | Watanabe, T. | Ueda, H. | Takehana, K. | Yamaguchi, M.
Although tumor necrosis factor (TNF) alpha is an important key factor in degeneration of equine superficial digital flexor tendon (SDFT), the dynamism of TNF receptors and associated factors on tendinocytes has not been elucidated. To reveal signaling events mediated by TNF-receptors (TNF-Rs) in tendinocytes, we focused on four signaling factors, TNF-R1, TNF-R2, TNF-Rassociated factor 2 (TRAF2) and nuclear factor-kappa B (NE-KappaB) , and investigated the distribution and production of these factors. Cultured tendinocytes were obtained from SDFTS of thoroughbred horses. The tendinocytes were treated with 10ng/ ml equine TNFAlpha medium for 6 hours and then the four factors on tendinocytes were visualized by using an immunohistochemical method, and the amounts of the four factors were determined by Western blot analysis. Although TNF-R1 and TNF-R2 co-localized on the same tendinocyte, in untreated control cells (normal condition), immunoreactivity against TNF-R1 was very weak but TNF-R2 showed a strong reaction. However, TNF-R1 showed the same high level of reaction as TNF-R2 in TNFAlpha-treated cells (inflamed condition). Intense TRAF2 and NF-KappaB were detected at inflamed condition, however both factors were also detected at normal condition. The distinct distributions of the four factors under different conditions (normal and inflamed condition) in uitro not only reflect the dynamism of the cytokines but may also provide important clues for a means to prevent from occurrence of tendonitis and progress of tendon degeneration.
Mostrar más [+] Menos [-]Increased concentrations of protein gene product 9.5 in the synovial fluid from horses with osteoarthritis
2001
Kitamura, H. (Hokkaido Univ., Sapporo (Japan)) | Okumura, M. | Sato, F. | Kimoto, K. | Kohama, M. | Hashimoto, Y. | Tagami, M. | Iwanaga, T.
Our previous study established protein gene product 9.5 (PGP 9.5), a ubiquitin C-terminal hydrolase, as a specific cytochemical marker of synovial lining cells (type B synoviocytes) in the horse joint. The present study aimed to detect PGP 9.5 in the synovial fluid and shows that PGP 9.5 is a valuable marker of osteoarthritis in the horse. Immunohistochemical staining confirmed rich and consistent localization of PGP 9.5 immunoreactivity in the cytoplasm of synovial lining cells in the normal horse joint. Western blot analysis of synovial fluid from normal joints could detect a significant band corresponding to that contained in the brain and synovial membrane extracts. When 60 synovial fluid samples from normal and abnormal joints were assayed with an enzyme-linked immunosorbent assay (ELISA) system, the concentration of PGP 9.5 tended to be elevated in osteochondrosis dissecance, inflammatory arthropathy and intra-articular fracture, among which a statistiture and the control. Thus, this study demonstrated the possibility that PGP 9.5, derived from synovial lining cells, may be a new biochemical marker for arthritic disorders of the horse.
Mostrar más [+] Menos [-]A rapid and highly sensitive method for diagnosis of equine influenza by antigen detection using immuno-PCR
2001
Ozaki, H. (Hokkaido Univ., Sapporo (Japan)) | Sugita, S. | Kida, H.
Detection of equine immunoglobulin-secreting cells by a plaque assay
1992
Goto, I. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Kamada, M. | Inaba, M. | Maede, Y.
Difference of virulence in causing metritis in horses between heavily encapsulated, less heavily encapsulated and non-capsulated strains of Klebsiella pneumoniae capsular type 1
1987
Kikuchi, N. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Hiramune, T. | Taniyama, H. | Yanagawa, R.
Cellular architecture of the synovium in the tendon sheath of horses: An immunohistochemical and scanning electron microscopic study
2002
Kohama, M. (Hokkaido Univ., Sapporo (Japan)) | Nio, J. | Hashimoto, Y. | Iwanaga, T.
Antigenic variation among equine H 2 N 8 influenza virus hemagglutinins
2001
Ozaki, H. (Hokkaido Univ., Sapporo (Japan)) | Shimizu Nei, A. | Sugita, S. | Sugiura, T. | Imagawa, H. | Kida, H.
Immunohistochemical demonstration of chromogranin A in endocrine organs of the rat and horse by use of region-specific antibodies
2001
Hashimoto, Y. (Hokkaido Univ., Sapporo (Japan)) | Ohki, H. | Sato, F. | Yanaihara, N. | Iwanaga, T.
Chromogranin A (CgA) is an acidic glycoprotein that is co-stored with hormones or neurotransmitters in granular components of endocrine cells and neurons, and released together with them in response to adequate stimulation. In addition to acting as a packaging protein. CgA functions as a precursor molecule that yields several bioactive peptides by proteolytic cleavage. The purpose of this study is to elucidate how different the processing of CgA is among endocrine tissues by immunostaining using multiple region-specific antisera, and to evaluate the availability of region-specific antisera. When various endocrine organs of rats were immunostained with four regionspecific antisera against rat CgA (CgA 1 - 28, 94 - 130, 296 - 314, and 359 - 389), all amine / peptide-secreting endocrine tissues except the pineal body were stained positively The adrenal medulla and gastric endocrine cells were equally intensely immunoreactive to all four antisera, while the other endocrine tissues, represented by pancreatic islets, showed different staining patterns depending on the antiserum. These results suggest that the processing of CgA differs from tissue to tissue. An antiserum against horse CgA 335 - 365, corresponding to rat CgA 359 - 389 which shows the highest concentration in the plasma and urine of the rat, again stained all endocrine tissues of the horse except the pineal body. Therefore, the anti-horse CgA 335 - 365 serum is useful for immunohistochemical survey of horse CgA, and may make possible the establishment of a CgA assay system for the measurement of CgA in the plasma, urine and saliva.
Mostrar más [+] Menos [-]A case of multilocular echinococcosis in a horse
1984
Miyauchi, T. | Sakui, M. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Ishige, M. | Fukumoto, S. | Ueda, A. | Ito, M. | Ohbayashi, M.