BACKGROUND: Infection by infectious bursal disease virus (IBDV) in turkeys may  lead to immunosuppression effects and therefore turkeys could not resist against pathogenic or less pathogenic agents. OBJECTIVE: The aim of present study was to examine the effects of IBDV on response of turkey’s poults to avian influenza virus (AIV).METHODS: A total of 100 day-old poults were divided randomly into 4 equal groups. Groups 1 and 2 were infected with 104CID50 of IBDV by oral route at 1 day of age; groups 1 and 3 were infected with 106 EID50 of AIV (H9N2) by the oculo-nasal route at day 30. Poults of  group 4 were kept  as uninfected control group. All groups were vaccinated with Newcastle disease virus (NDV) vaccine. Blood  samples  via wing web were collected at days 0, 30, 37, 44, 51  and 58 and anti- NDV and anti-AIV serum titers were measured by HI test. At days 33 and 41 three poults of each group were euthanized and their splenic lymphocytes proliferation repose to phytohaemagglutinin was assessed. RESULTS: Influenza clinical signs were prolonged and more intensive in group 1 than group 3. The mean HI titers to NDV were significantly lower in group 1 than group 3, in all sampling times, but anti-AIV titers were significantly lower in group 1 compared to group 3  from days 14 AIV (H9N2) post infection. The lymphocyte proliferation assay with PHA did not show any differences between groups 1 and 3. CONCLUSIONS: IBDV suppresses immune response in turkey and causes prolonged and more intensive clinical signs after challenge with AIV.

A total of 60 one-day old Ross broiler chicks were used in this study. The birds were divided into four groups. The first group A was vaccinated against IBD with a Cevac IBD L vaccine at one-day old by intra-yolk sac method (lYS) with a dose of 0.5ml using a (1ml) syringe, whereas the second group B was vaccinated against IBD with the same vaccine at 14 days of age by drinking water method (DW). The third group C and fourth group D were considered as a positive and negative control respectively . On day 35, the birds were weight and killed to collect blood samples and lymphoid organs were removed and weight to study the effects of (lYS) rout on body weight , lymphoid organ weights and the humeral immune response to IBD vaccine which measured by ELISA test. The results showed significant differences at (P≤0.05) in body weight of group A, in addition significant (p<0.05) increase in the all lymphoid organs weight , Bursa of fabricius (BF) was 1.643 a ±0.066 in group comparison with other groups which had been recorded 1.102 b ±0.022, 0.850 c ±0.067 in group B and C respectively. Group B indicated that was a significant decrease at (p<0.05) in the weight of of spleen which was 1.102 b ± 0.022 in comparison to control groups. With regard to serological test, bird from group A recorded higher Ab titer 24038 a± 685 compared to those of other groups , and this increment was statically significant (p<0.05), however both group A and B revealed a53 significant differences( p <0.05) from the control groups. Generally the results indicated that intra-yolk sac method was highly effective rout of IBD vaccine administration

IBD is a highly contagious viral disease which inhibit the immune system ofchickens, which is responsible for major economic losses within the poultry industriesworldwide. A total of 80 samples were collected from 8 different broiler farms fromQurna, Midyanah, Qarmat Ali, Zubair. Upon the clinical finding and postmortemlesions of the necropsied birds; bursa of fabricius, kidney, Junction between gizzardand proventriculus and thigh muscles were processed for histopathological andmolecular detection with PCR. The clinical signs were depression, pasty vent andwhite dropping. Various overall changes are observed in the bursa, such as swelling,hemorrhages to atrophy in size; In addition, hemorrhages were seen in the thighmuscles. The histopathological changes of Bursa of fabricius showed follicularvacuolation and vascular congestion ;multiples degrees of hemosiderin deposition,and the edema accumulate between follicles and basement membrane ; also, showedmoderate infiltration of inflammatory cells and accumulation of inflammatory cellslymphocytes macrophage , plasma cells as well to showed a congestion of the bursalcapsule, kidney microscopic findings renal vascular congestion in the cortex and alsothe medullary area along with the vacuolar degeneration. The suspected tissuesamples were assayed using RT-PCR for IBDV targeting VP2 gene. Out of the testedsamples 15 were Positives. In spite of using IBD vaccines in different farms of thestudied areas; the present study was detected IBD in different areas of Basrahprovince by PCR and mentioned clinical and histopathological finding, therefore it'snecessary to study the sequence analyses of such disease in future.

Objective: This study was carried out to evaluate the effect of garlic (Allium sativum) with its immunomodulatory activity, on the susceptibility of broilers to infectious bursal disease, being an immunosuppressive disease. Materials and methods: Day-old broilers (102) were separated into 6 groups A-F of 17 each. Groups A, B and C had 0.125% of garlic-meal in feed. At 8 and 18 days of age groups A, C, D and F were administered IBD vaccine and groups B, C, D and E were infected with 1LD50 IBD virus (10-3.4 in 0.08ml PBS) via conjuctival instillation at 4 week-old. Clinical signs, mortality and gross pathological lesions were scored. Histopathological lesions in bursae of Fabricius were recorded. Virus antibody titre in serum was assayed at 1 day-old, 4 and 6 weeks-old using quantitative agar gel immunodiffusion test. Data generated was analysed using descriptive statistics, ANOVA and Duncans multiple comparison tests (P [J Adv Vet Anim Res 2018; 5(3.000): 275-281]

Objective: This study was conducted to assess the effect of dietary Moringa oleifera leaf (MOL) feed supplementation on serum biochemical parameters of broilers challenged with very virulent infectious bursal disease virus (vvIBDV). Materials and methods: Two hundred and forty day-old Ross 308 hybrid broiler chicks were randomly assigned into four groups (A, B, C and D) of 60 chicks each and raised in deep litter housing. Broiler starter (BS) and broiler finisher (BF) mash were formulated each with 5% MOL included as part of the feed ingredient for broilers in groups A and B while BS and BF for broilers in groups C and D were formulated without MOL. Broilers in groups A, B and C were challenged intraocularly at 35 days of age with with 0.05 mL of a live vvIBDV, while those in group D served as control. Blood was collected from 10 broilers in each group via the wing vein at 35, 38 and 42 days of age to determine their serum biochemical profile. Results: The level of melondialdehyde (MDA) was observed to significantly decrease in groups A and C. There was a significant decrease in the level of AST in group A, B, C and D. The values of ALT significantly decreased in group A, B, C and D. Conclusion: Supplementing broilers feed with MOL neither protect the liver from damage nor prevent lipid peroxidation. [J Adv Vet Anim Res 2018; 5(2.000): 155-165]

Objective: A cross sectional study was conducted to estimate the prevalence of Infectious Bursal Disease (IBD) and Newcastle Disease (ND) in poultry of Narsingdi district, Bangladesh. Materials and methods: Post mortem of a total of 208 dead birds were done for the diagnosis purpose. Different poultry species included in this study included broilers, layers, pigeon, duck etc. Results: Among the examined birds, 38% were found to be affected with IBD, and 11% were affected with ND. Age of the birds for both IBD (19.95; 95%CI: 16-23) and ND (122.23; 95%CI: 98.62-145.83); and flock size only for IBD (1317; 95%CI: 1175-1460) was found significantly associated. The chicks aging between 16-23 days and flock size between 1175-1460 were found to be the most susceptible group to IBD, and adult poultry (98.62-145.83 days old) was mostly susceptible to ND. Conclusion: IBD and ND are highly prevalent in the study area. Therefore, it is necessary to conduct effective control measures to reduce the prevalence of these diseases. This study can help in designing appropriate control measures considering risk factors of these diseases. [J Adv Vet Anim Res 2016; 3(4.000): 406-412]

Objective: The objective of this study was to identify the current status of emerging diseases of pet animals in Bangladesh and to estimate the percentage of disease occurrence in the pet animals. Materials and methods: A 2-month potential study was conducted to estimate the percentage (%) of disease occurrence and current status of emerging diseases of pet animals in Bangladesh. A total of 550 animals were examined and 358 data were collected from the disease registered book of the Central Veterinary Hospital (CVH), Dhaka and SA Quaderi Teaching Veterinary Hospital, Chittagong Veterinary and Animal Sciences University (CVASU), Bangladesh during June and September, 2014. The data were analysed, and the results on the newly emerging diseases were expressed as percentage (%). Results: Among the most general pet animals in Bangladesh (dog, cat, rabbit), the mostly occured diseases were scabies (23.07%), feline ascariasis (37.14%) and rabbit mange (34.61%), while the less frequent diseases were canine parvovirus enteritis (2.19%), cat scratch disease (5.71%) and overgrown teeth (7.69%). Conclusion: The study provides basic information about the current status and the percentage (%) of disease occurrence considering the emerging diseases of pet animals in Bangladesh. [J Adv Vet Anim Res 2016; 3(4.000): 413-419]