The present study was aimed to investigate the gynaeco-pathological disorders by post-mortem and histopathological examination, and to identify the associated bacteria. A total of 310 genital tracts of cows were collected from slaughter house of Dinajpur Sadar Upazilla during April 2009 to March 2010. Among the 310 samples, 31.29% (n=97/310) were affected with endrometritis. Similarly, 8.37% (n=26/310) cystic ovary, 6.77% (n=21/310) ovary hyperplasia, 4.84% (n=15/310) pyometra, 4.84% (n=15/310) parovarian cyst, 4.52% (n=14/310) hydrometra, 4.84% (n=15/310) ovary hypoplasia, 3.55%(n=11/310) ovaro-bursal adhesion, 1.29% (n=4/310) vaginal cyst and 0.66% (n=2/310)) hemorrhagic uterine horn were detected by post-mortem examination, the cases were reconfirmed by histopathological studies. Uterine fluid (n=50) samples were collected, and were subjected for conventional bacteriological culture and biochemical analysis. Escherichia coli and Salmonella sp. could be identified from 30% (n=15/50) and 8% (n=4/50) samples, respectively. In conclusion, various pathological disorders in the female reproductive system of cows are prevalent, that may cause reduction of calf production.

Duck plague (DP) is the most feared duck disease in the world. For isolation, identification, molecular detection and characterization of DP virus (DPV), a total of 94 samples were collected from commercial farms (n=6) and households (n=13) from Rajshahi (n=37), Netrokona (n=35) and Mymensingh (n=22) districts of Bangladesh. The samples were processed and inoculated into 11-13 days old embryonated duck eggs for virus propagation. Virus was identified using agar gel immunodiffusion test (AGIT) and passive hemagglutination (PHA) test, and was confirmed by polymerase chain reaction (PCR) targeting DNA polymerase and gC genes, followed by sequencing. Pathogenicity tests were performed using duck embryos, ducklings and ducks. Among the 94 samples, 17 isolates were confirmed as DPV by PCR amplification of partial DNA polymerase (446-bp) and gC genes (78-bp), respectively. One of the isolates (Anatid herpes 1 BAU DMH) was sequenced and found to be closely related with a Chinese variant of DPV (GenBank: JQ647509.1). Thus, we assume that both Bangladeshi and Chinese isolates of DPV may have a common ancestor. [J Adv Vet Anim Res 2015; 2(3.000): 296-303]