Evaluation of antagonistic activity of strains of lactic and bifid bacteria, metabolite producers, as well as the treatment and prophylactic efficacy of Laktimet and Batsinil probiotics was realized in the conditions of the Republic of Belarus. In course of study there were estimated 29 strains of lactic acid bacillus of different kinds, 13 strains of bifid bacteria and 35 strains of bacillus (Bac. subtilis and Bac. pumilis). There were studied 3 strains of bifid bacteria: Bifidobacterium adolescentis MS-42; Bifidobacterium adolescentis BIM V-01; Bifidobacterium adolescentis BIM V-87; 2 strains of lactic acid bacillus: Lactococcus lactis subsp. Lactis BIM V-132, Lactococcus casei subsp. rhamnosus BIM V-189; and 6 strains of Bacillus subtilis. Research results showed that the main part of the studied strains had steady antagonistic activity to test-cultures of opportunistic pathogenic bacteria. Zone of growth delay of test-cultures of the main quantity of strains (from 54 up to 92%) was from 11 up to 30. Research results showed that application of cell-free probiotics Laktimet and Batsinil promoted the increasing of resistance by means of increasing or lowering of total protein, insignificant increasing of blood serum bactericidal activity and increasing of phagocytic activity of leukocytes on 7,3%

Investigation of immunity of broiler chickens of Kobb-500 cross vaccinated by national associated viral vaccine and foreign (ABIC, Israel; Vitex XXI vek Seva) vaccines against Newcastle disease and infectious bronchitis was investigated in the conditions of the Republic of Belarus. In course of studies there was analyzed the content of immunoglobulin in blood serum, as well as the influence of living vaccines on immunity formation. Research results showed that broiler chickens immunization against Newcastle disease and infectious bronchitis by national and foreign vaccine produced by ABIC company made it possible to increase the phagocytic activity of pseudoeosinophils, bactericidalal and lysozyme activity of blood serum, that proved the activation of nonspecific protection factors. At the same time in blood serum there was stated the increasing of immunoglobulin number in 1,5-3,0 times in comparison with control groups. Economic efficiency of all analyzed vaccines was analyzed

After immunization of 30-35 day old piglets against a salmonellosis, pasteurellosis and streptococcosis (SPS) with special produced in the Republic of Belarus SPS vaccine in combination with immunostimulant sodium thyosulfate and vitamin C there was observed an activation myeloblastic hemopoiesis and lowering of erythropoeisis.

Morphological and structural changes in bone marrow of piglets which were immunized with produced in the Republic of Belarus SPS vaccine (against salmonellosis (Salmonella), pasteurellosis (Pasteurella) and streptococcus) in combination with immunostimulants and without them were analyzed in the conditions of the Republic of Belarus. Piglets of the first group were vaccinated against salmonellosis, pasteurellosis and streptoccocusis by SPS vaccine. Piglets of the second group were vaccinated by SPS vaccine with vitamin C. Piglets of the third group were vaccinated by SPS vaccine with immunotstimulant sodium thiosulphate. The fourth (control) group of animals was administrated with normal saline. Animals were immunized twice, intramuscularly with 7 day interval in dose of 4 ml (primary), and 5 ml (secondary). Vitamin С was administrated in dose of 0,05g per head. Sodium thiosulfate was administrated in combination with the vaccine in 30% concentration. Alongside with myelogram there were derived formulas of different cell groups of bone marrow: leucoerythroblastic index; intramedullary index of neutrophil maturity; intramedullary index of eosinophile maturity. Detailed myelogram of piglets on the 14-th day after the second immunization was presented. Research results showed that in all groups of vaccinated animals there was noted the activation of myeloblastic hematogenesis and decreasing of erythropoeisis. Piglet immunization was accompanied by strengthening of myeloblastic hematogenesis, increased number of lymphocytes and plasma cells in bone marrow

Studying of efficiency of application of modified scheme of hyperimmunization of bull-producers with a designer salmonella polyantigenin in comparative analysis with the production scheme of hyperimmunization was realized in the conditions of the Republic of Belarus. Research results showed that engineered polyvalent antigen for hyperimmunization of producer bulls contained in their formula the following serovariants of salmonella in 1:1 ratio: S. typhimurium 371, S. dublin 373, S. choleraesuis 370, and S. enteritidis CB. The obtained antigen was sterile, harmless and had pH 7,3. Hyperimmunization of producer bulls in the experimental scheme revealed in increasing of leukocytes quantity on 57,3 %, banded neutrophils on 86,7 %, B-lymphocytes - on 42,2 %, content of total protein - on 25,6 %, content of Ig G - on 77,8 %, lg M - on 51,6%. After the fifth administration of antigen there was stated the lowering of agglutinating activity of blood serum on 25%, content of Ig G - on 54,4%, Ig M - on 14,6 %, that indicated to the inefficiency of antigen administration and became the basis for termination of hyperimmunization cycle. Immunobiological reaction of bull-producers which were hyperimmunized by the suggested scheme differed from reaction of animals which were hyperimmunized by the production scheme: content of total protein increased on 10,1%, protein content - on 27,3 %, content of lg G - on 24,8 %, lg M - in 23,7%

The physical and biological properties of a produced in the conditions of the Republic of Belarus nonspecific blood serum of animals according to an improve technology corresponded to all necessary standards. The prophylactic efficiency of nonspecific blood serum of animals was 80%, that was on 10% higher than serum of convalescents. Therapeutic effectiveness of nonspecific blood serum was 90%, that was on 8% higher than serum of convalescents

Studying of palatability of bait blisters applied for oral immunization of wild carnivores against rabies was realized in the conditions of the Republic of Belarus by an example of foxes which were administrated with liquid viral vaccines against rabies. Foxes were divided into 5 groups with 8 animals in each. Animals of the first group were immunized with a liquid viral vaccine in blister baits Lisvulpen-VBF which was produced from an attenuate strain of rabies virus SAD-BERN (Belarus). Animals of the second group were immunized with liquid viral vaccine Belvak TM BP produced from the cultural living strain KMIEhV-V101 (Belarus). Animals of the rest three groups were immunized with liquid viral vaccine in blister baits which was produced from a modified strain of a fixed attenuate rabies virus KMIEV-94. Foxes of the forth group were additionally fed with ascorbic acid with baits in the doze of 0,1 g, and foxes of the fifth group were fed with 5 ml immune response modifier Nuklevit. All mentioned blister baits were studied in accordance with the following parameters: bait flavor and attraction to animals; form and size of bait; bait consistency; blister and its size in relation to bait; palatability of bait; degree of damage of bait containing a vaccine; quantity vaccine which was got into animal oral cavity; postvaccinal complication. Palatability of liquid viral vaccines in blister baits Lisvulpen-VBF was 96%, Belvak TM BP - 94%, KMIEV-94 - 80%

Influence of potassium orotate on hematological, immunomorphological indexes and and marrowy myelopoiesis of broiler chickens in the process of oral associated immunization against infectious bronchitis and poultry Newcastle disease was investigated in the conditions of the Republic of Belarus. There were studied 60 broiler chickens divided into 3 groups: one control group and two experimental ones. Research results established that potassium orotate application in combination with associated poultry immunization against infectious bronchitis and Newcastle disease increased the number of leucocytes and the volume of adenoid tissue as well as raised the lymphocytes density in thymus and bursa of Fabricius. In the bone marrow aspirates in 7 days after immunization in the control group there was stated the increasing of basophilic myelocytes number in comparison with the second experimental group. In 14 days in the control group there was noted the increasing of a total number of basophiles. In 21 days after immunization there were stated no considerable changes in the composition of basophil cells in the experimental groups. Research results showed that potassium orotate intensively stimulated the development of plasmocytic reaction in the conditions of multipartial immunization

Experimental research on production of antigen preparation for hyperimmunization of bulls from blood of which it was possible to produce an active medical and preventive serum against cattle salmonellosis and pasteurellosis was realized in the conditions of the Republic of Belarus. There were developed four variants of antigen on the basis of the formolated aluminous concentrated vaccine against calf salmonellosis and on the basis of semiliquid aluminum hydroxide vaccine against cattle pesteurellosis. All obtained variants of associated antigen had concentration of hydrogen ions, which was close to the neutral rating; they were also sterile and safe for white mice. Associated antigen which was constructed from vaccines in the ratio of 1:1, 1:2, 1:3, and 2:3 had an ability to raise the protection of immunized experimental guinea pigs in the conditions of controlled introduction of infection with S. dublin 373 and S. tythimurium 371. Pasteurellosis component of associated antigen in 1:1 and 1:2 variants protected against death only 3 out of 5 immunized doves, both in relation to P. multocida strain N 796, and bacteria P. multocida strain N 5264. Vaccine ratio in the associated antigen 1:3 and 2:3 turned to be more immunogenically balanced: all tested doves survived after introduction of infection of broth culture P. multocida of N 796 and N 5264 strains. Experimental results showed that the associated antigen on the basis of vaccines in ratio of 1:3 was sterile, safe, active and suitable for hyperimmunization of bull producers serum against salmonellosis and pasteurellosis of calves, as well as antigen in the variant 2:3, but this ratio increased the expenses

Economic efficiency of immunization of piglets with a vaccine against a salmonellosis, pasteurellosis and streptococcosis in combination with 20% solution of sodium thiosulfate and Vitamin С as immune stimulators was analyzed in the production conditions of the Republic of Belarus.