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Experimental transmission of bovine leukemia virus in cattle via rectal palpation
2006
Kohara, J.(Hokkaido. Animal Research Center, Shintoku (Japan)) | Konnai, S. | Onuma, M.
We examined whether Bovine leukemia virus (BLV) was transmitted by rectal palpation using a common sleeve between a BLV-infected cow and BLV- negative steers. Three of four steers developed antibodies against BLN as determined by agar-gel immunodiffusion (AGID) test between 7 to 10 weeks after the first rectal palpation using common sleeves from BLV-infected cow. In the steers, BLV proviral DNA were detected by PCR 1 to 5 weeks earlier than detection of the antibodies by the AGID test. Our experiments demonstrated that rectal palpation is a potential cause of BLV spread in herds and that detection of BLV proviral DNA in cattle by PCR is useful screening test for early diagnosis of BLV infection.
Mostrar más [+] Menos [-]Effect of neutralizing monoclonal antibodies on Hantaan virus infection of the macrophage P388D1 cell line
1992
Yao, J.S. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Arikawa, J. | Kariwa, H. | Yoshimatsu, K. | Takashima, I. | Hashimoto, N.
Report on the prevalence and experimental infections of Angiostrongylus siamensis Ohbayashi, Kamiya et Bhaibulaya, 1979, parasitic in the mesenteric arteries of rodents in Thailand
1980
Kamiya, M. | Oku, Y. | Katakura, K. | Kamiya, H. | Ohbayashi, M. | Abe, H. | Suzuki, H. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine)
Mitogenic responsibilities of lymphocytes in canine babesiosis and the effects of splenectomy on it
1987
Kawamura, M. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Maede, Y. | Namioka, S.
Distribution of bovine immunodeficiency virus in the organs of experimentally infected cows
1997
Tajima, M. (Hokkaido Univ., Sapporo (Japan)) | Sato, N. | Kirisawa, R. | Onuma, M. | Maede, Y.
The distribution of bovine immunodeficiency virus (BIV) in the organs of experimentally infected cows was investigated by use of nested polymerase chain reaction (PCR). Two cows (Nos. 1 and 2) experimentally infected with BIV were alive without any clinical symptoms of BIV infection for 28 months. Viral and proviral genomes of BIV were continuously detected from peripheral blood leukocytes in those cows by nested PCR. Proviral genome of BIV were also detected in liver, lung, and spleen cells in the two cows, and in the brain in cow No.1. Viral genomes were detected in liver, lung and spleen cells in cow No.1, and detected only in spleen cells in cow No.2. These results suggest that BIV tended to the persistent in some organs, especially in the spleen
Mostrar más [+] Menos [-]Bacterial infection which produces high mortality in cultured Japanese flounder (Paralichthys olivaceus) in Hokkaido
1987
Kodama, H. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Murai, T. | Nakanishi, Y. | Yamamoto, F. | Mikami, T. | Izawa, H.
