Edwardsiella tarda was recovered from cultured freshwater catfish (Claries gariepinus) with a prevalence rate of 0.42% in summer season. Experimental infectivity studies of E. tarda in Catfish and Tilapia nilotica were done using the intraperitoneal (I/P) and intramuscular (I/M) routes. Experimentally infected Catfish with a dose of 0.3 ml from E. tarda bacterial suspension of 108 CFU/ml manifested pathognomonic clinical signs and postmortem lesions than the experimentally infected Tilapia nilotica. Application of PCR technique for rapid detection of E. tarda infection in the experimentally infected fishes gave positive reaction in 4th day post infection in catfish and the 7th day post infection in Tilapia nilotica.

Abortion, still birth, premature birth and mortality of cross bred dairy cattle (Jersey × Tharparkar/Red Sindhi) were noticed in the organized dairy farm of National Dairy Research Institute, Eastern Regional Station, Kalyani, Nadia, situated in hot and humid climatic area nearer to the river Ganges of West Bengal, India. The history of the farm revealed newly introduction of pure bred dairy cattle and outbreak of FMD during mid March to mid April, affected about 34% cross bred cows. During investigation, intermittent rise of temperature (104°F -108°F), anorexia, rapid respiration, progressive deterioration of health of animals and loss of milk production were also noticed. On the basis of past history, twenty suspected animals were taken for disease investigation. Repeated visit of the farm and repeated examinations of blood smears were done to observe any haemoprotozoan infections. Twenty to thirty percent of those suspected animals were found positive for Brucella antibodies by STAT, plate agglutination test and MRT. After a massive screening of blood smears, during the visit of third time, ultimately one animal (Identification number JT614) was found positive for the presence of Trypanosoma evansi infections in Giemsa stained blood smears. The infected and all suspected animals were successfully treated with single injection of a mixture of quinapyramine sulphate and chloride @ 7.4 mg/kg body weight subcutaneously. As a prophylactic measure, a mixture of quinapyramine sulphate and chloride @ 7.4 mg/kg body weight subcutaneously were also administered to all suspected animals prevented further occurrence of the disease in this dairy farm. It can be concluded that the iAfection with T. evansi in this farm has happened in a condition of intercurrent diseases with environmental stresses.

One hundred thirty one samples (nasal, faecal, soil, tissue from dead foal) were tested for presence of Rhodococcus equi. These samples included 58 nasal swabs including 45 from foals with respiratory problem and 13 from in contact apparently healthy foals. Faecal samples were 54 including 41 from foals with respiratory problem and 13 from in contact apparently healthy foals. Faecal and nasal samples were from same foals, soil samples from infected premises were 15, besides tissues from foals (4) which died due to respiratory problems. Fourteen isolates of Rhodococcus equi were obtained from foals with respiratory problems, which were subjected to in vitro antibiotic sensitivity testing to 17 antimicrobial agents which were amoxycillin, gentamycin, ampicillin, trimethoprim, chloramphenicol, sulphadiazine, cloxacin, oxytetracycline, amikacin, streptomycin, cotrimoxazole, cephalexin, kanamycin erythromycin, ciprofloxacin, neomycin and rifampicin. All the isolates were found sensitive to chloramphenicol, erythromycin, oxytetracycline, ciprofloxacin, neomycin and rifampicin.

A total of 128 faecal samples of dogs suspected for canine parvovirus infection were examined by Haemagglutination (HA) and Haemagglutination inhibition (HI) tests. The prevalence of canine parvovirus infection was 45.30% (58/128) and the age wise maximum (70.20%) prevalence was found in dogs upto 6 month. The breed wise maximum (56.90%) prevalence was in Non-descript dogs and sex wise prevalence revealed no significant difference in male (45.9%) and female (43.3%).

Firfty serum and fifty semen samples collected from cattle and buffalo bulls were subjected to ELISA and gB gene based PCR, respectively to detect antibodies in serum and viral DNA in the semen against BHV 1. Out of 50 bulls, 15 (30%) serum samples were detected positive by ELISA while 21 (42%) semen samples were positive by gB gene based PCR. While correlating the results of ELISA and PCR, some seronegative bulls revealed presence of viral genome in semen whereas few seropositive bulls could not reveal viral genome in semen, thus, suggesting application of combined serological assay and PCR assay to detect the presence of BHV-1 infection in bulls.

Hyalomma ticks of either sex were collected from the apparently healthy cross-bred cattle. The ticks were dissected and their salivary glands were stained by methyl green pyronin stain for the detection of Theileria annulata sporozoite in their salivary glands. The prevalence of Theileria annulata infection in Hyalomma ticks was found to be 21.5%. No correlation was found between parasitemia of cattle and tick infection rate.

Production examination of efficiency of concentrated vaccine against swine erysipelas from matrix Konev of trial series, control of its immunizing power and production experiments of biological preparation were realized in the conditions of the Republic of Belarus. Research results showed that the presented vaccine had more expressed immunogenic properties in comparison with its production analogue and provided piglets protection against infection. There was analyzed the immunological efficiency of the vaccine: determination of quantity of erythrocytes; leukocytes; content of hemoglobin, level of lysozyme activity, bacterial growth-inhibitory activity, blood serum, phagocytic activity, neutrophilic leukocytes, content of total protein and protein fractions, titre of erysipelas antibodies, and preventive properties of blood serum. Efficiency of biological preparation was determined on the basis of analysis of disease incidence rate, loss of immunized swine caused by swine erysipelas

Evaluation of pathogenicity of Paenibacillus alvei culture obtained from a dead bee brood from Berezinsky Biosphere Nature Reserve was realized in the conditions of the Republic of Belarus by an example of 30-day old white male mice with body weight of 16-18 grams. Experimental animals were infected with European foul brood (Paenibacillus alvei) intraperitoneally. Research results showed that bacteria Paenibacillus alvei after the intraperitoneal infection with various amount of microbial cells did not cause mouse death; i.e. it was not pathogenic for white mice. In course of the realized observations over the laboratory mice after introduction of infection there were stated no changes in mice behavior and overall condition. There were no visible pathologicoanatomic changes in internal organs. After the bacteriologic examination of internal organs and blood there were isolated pure cultures of Paenibacillus alvei