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Functional enucleation of mouse metaphase II oocytes with etoposide
1998
Elsheikh, A.S. (Hokkaido Univ., Sapporo (Japan)) | Takahashi, Y. | Katagiri, S. | Kanagawa, H.
Mouse metaphase two (M two) oocytes were exposed to 50 mug/ml etoposide (ETO) before and after parthenogenetic activation with 7% ethanol and they were washed with 0.75 M sucrose. The ETO treated parthenogenetically activated oocytes were cultured or fused to single blastomeres of late 2-cell stage mouse embryo to test their ability to support development in vitro. In parallel untreated parthenogenetically activated oocytes were cultured to serve as control. None of ETO treated oocytes developed beyond the 2-cell stage, whereas 4% of the reconstituted embryos and 35% of control developed to blastocysts. It is concluded that mouse M two oocytes can be functionally enucleated by ETO treatment and can be used for nuclear transfer experiments
Mostrar más [+] Menos [-]Эффективность применения мультиэнзимной композиции Фекорд – 2004С и биологически активной добавки Оксидат торфа в кормлении поросят
2009
Kapanskij, A.A., Grodno State Agrarian Univ. (Belarus)
Results of researches on studying influence separate and combination feeding fermented compositions Fekord-2004C and biologically active additive Oxide Peat on energy of growth and protective properties of an organism of pigs – deprivation are stated. It is established that enrichment of mixed fodder investigated by the fodder fermented additive Fekord-2004C at the rate of 0,15 kg on ton of mixed fodder in combination application with biologically active substance Oxide Peat in a doze of 1,0 kg on ton of mixed fodder promotes increase of growth rate of pigs on development and to decrease of the charge of forages on unit of a gain of alive weight and safety of pigs
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