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Direct Detection of Cryptosporidium spp. In Cattle In Karbala Province and its Environs, Iraq Texto completo
2021
Alali, Firas | Jawad, Marwa | kh.k.Al-khayat, Kadhim
Gastrointestinal diseases are caused by many of the apicomplexan protozoan parasites and cause significant clinical diseases. Cryptosporidiosis is one of the most important diseases of young ruminant livestock, particularly neonatal calves. This study investigates the prevalence of Cryptosporidium infection in Karbala farm cattle. Totally, 1360 fecal samples were collected from cows and calves between (1 week to 5years old) from both genders, from different regions of Karbala. Different fecal examination methods (direct fecal smear, fecal flotation with saturated salt solution and faecal smear stained by modified Ziehl-Neelsen were performed to determine Cryptosporidium spp. oocysts and other mixed parasites in the fecal samples. The results showed that 29.4% of the fecal samples were detected positive for cryptosporidiosis (400/1360). The highest infection rate appeared (41.9%) in the age group from 3-6 months old, and less rate was (15.1%) in 2 years (P≤0.05). Additionally, there was no significant variation found in infection rates between males (33.8%) and females (25.6%). Mixed infections have been reported with Eimeria spp. oocysts (7.3%), Giardia lamblia cysts (1.4%) and Trichostrongylus spp. eggs (5.1%) (P≤0.05). The results indicated the requirement for the eradication of the gastrointestinal parasites by deworming and a good management system in the sampled region.
Mostrar más [+] Menos [-]New insights into the prevalence and phylogenetic diversity of Cysticercus ovis isolates in sheep from Sulaymaniyah, Iraq Texto completo
2021
Ahmad Mohammed Aram
New insights into the prevalence and phylogenetic diversity of Cysticercus ovis isolates in sheep from Sulaymaniyah, Iraq Texto completo
2021
Ahmad Mohammed Aram
Although ovine cysticercosis is not a zoonotic problem, it results in substantial economic losses due to the condemnation of infected tissues or entire carcasses. This study aimed to record preliminary data on the prevalence, and phylogenetic diversity of Cysticercus ovis isolates from slaughtered sheep in the province of Sulaymaniyah, Iraq.
Mostrar más [+] Menos [-]New insights into the prevalence and phylogenetic diversity of Cysticercus ovis isolates in sheep from Sulaymaniyah, Iraq Texto completo
2021
Although ovine cysticercosis is not a zoonotic problem, it results in substantial economic losses due to the condemnation of infected tissues or entire carcasses. This study aimed to record preliminary data on the prevalence, and phylogenetic diversity of Cysticercus ovis isolates from slaughtered sheep in the province of Sulaymaniyah, Iraq. From January to September 2020, 6, 411 slaughtered sheep were examined for C. ovis by routine meat inspection. The amplification and sequence analysis of the COX1 gene for up to 35 specimens of C. ovis was performed using conventional PCR. The overall prevalence rate was 1.3%, and the prevalence was significantly higher in older sheep (>1 year) than younger ones (<1 year) (P< 0.05). The cardiac muscle showed a higher tendency to carry C. ovis infection compared to other examined muscles. Sequence analysis of the COX1 gene revealed six haplotypes, and the level of pairwise nucleotide diversity between individual haplotypes was 1–2%. Five out of six of the Taenia ovis haplotypes recovered could have been recorded for the first time globally. Phylogenetic interpretation indicated that all the T. ovis haplotypes clustered in a single clade, and it also indicated an extremely close similarity to Iranian and New Zealand isolates. Globally, this report adds new data on C. ovis genetic diversity, which provide an extremely useful molecular background with regard to future preventive as well as control strategies.
Mostrar más [+] Menos [-]Molecular study of Cysticercus tenuicollis from slaughtered sheep in Sulaymaniyah province, Iraq Texto completo
2020
Mohammed, Aram Ahmad
Molecular study of Cysticercus tenuicollis from slaughtered sheep in Sulaymaniyah province, Iraq Texto completo
2020
Mohammed, Aram Ahmad
Cysticercosis caused by the larval stage of Taenia hydatigena is economically the most important endemic parasitic disease in Iraq. Few data are available relating to the genetic divergence of this helminth. This study aimed to molecularly characterise Cysticercus tenuicollis isolates from sheep in Sulaymaniyah province, Iraq. DNA extraction and amplification of specimens of C. tenuicollis from 46 sheep were conducted by PCR for the mitochondrial 12S rRNA gene. The 19 amplicons were subjected to purification and partial sequencing. Five 12S rRNA nucleotide sequence haplotypes were found. The pairwise nucleotide difference between haplotypes of 12S rRNA gene ranged from 0.2% to 0.7%. Four out of the five haplotypes of C. tenuicollis contained one to two base mutations and were discovered in Iraq for the first time, and this may be a unique mutation globally which has not been recorded previously. Three newly recorded haplotypes contained only one single mutation, and the other one contained two mutations. Phylogenetic analysis showed that all isolated strains were closely related to Iranian sheep isolates. Four new strains of T. hydatigena were discovered for the first time in the study area.
Mostrar más [+] Menos [-]Molecular study of Cysticercus tenuicollis from slaughtered sheep in Sulaymaniyah province, Iraq Texto completo
2020
Mohammed Aram Ahmad
Cysticercosis caused by the larval stage of Taenia hydatigena is economically the most important endemic parasitic disease in Iraq. Few data are available relating to the genetic divergence of this helminth. This study aimed to molecularly characterise Cysticercus tenuicollis isolates from sheep in Sulaymaniyah province, Iraq.
Mostrar más [+] Menos [-]Epidemiology and genetic analysis of Oestrus ovis from slaughtered sheep in Sulaymaniyah province, Iraq Texto completo
2025
Mohammed Aram Ahmad | Soor Taib Ahmed Hama
Oestrosis is a type of nasal myiasis that is caused by larvae of flies from the Oestrus genus and is a disease of economic significance in small ruminants. The research aimed to investigate the prevalence of oestrosis and detect differences in the COX1 gene among haplotypes found in Sulaymaniyah, in the Kurdistan region of Iraq.
Mostrar más [+] Menos [-]Seroprevalence of Crimean–Congo Hemorrhagic Fever in Cattle in Basrah Province, Iraq Texto completo
2024
Douaa Hashim
There is need to knowledge geographic distribution of CRIMEAN–CONGO HAEMORRHAGIC FEVER virus (CCHV) in Iraqi cities so this study take place in different region in Basrah to detect viral antibodies. Specific serological indirect ELISA using to determined IgG antibodies against CCHF virus. Blood samples were collected from large ruminants from buffalo, cows, and calves starting from June to the end of September 2023. Results showed presence of IGg antibodies were found at a rate of 42.44% from 172 cattle (73) positive. Titers of IGg was high among females compare male and highly antibody recorded in older animals 2-3 years compare with less than 2 years. According to the regions Abo-alkaseeb has been highly infected animals out of 57cases 33(64.7%) positive. Samples of hard and soft ticks were collected from Basrah governorate, were classified to the family Ixodidae ticks include three species, including: Rhipicephalus sanguineus, Hyalomma anatolicum and Hyalomma truncatum. The widespread type is the Hyalomma type. The presence of the virus has been found in animals in which there are more ticks than in animals that are tick-free. These results suggest that is (CCHV) widespread in the cattle populations southern of Iraq.
Mostrar más [+] Menos [-]Anti-lice activity of Citrullus colocynthis fruits against Pediculus humanus capitis in vitro Texto completo
2023
Anah, S.A.
The increasing resistance of head lice Pediculus humanus capitis to many drugs has highlighted the need for new alternatives to control head lice in adults. The effect of two types of extracts (aqueous and alcoholic) of Citrullus colocynthis fruit on adult lice was tested in vitro. The results showed that the alcoholic extract with a concentration of 20% showed similar efficacy in killing adult lice to that of Natroba 9% w/ w, with values ranging between 87% to 98% within 18 minutes, followed by a 20% aqueous extract with a 44% to 79% death rate. A 10% concentration of both types of extracts had moderate lethality for lice, while a 5% concentration did not show strong lethality for adult lice. These results revealed significant differences between the control group and those treated with alcoholic and aqueous extract concentrations of C. colocynthis fruits at the probability level p ≤ 0.05.
Mostrar más [+] Menos [-]DIAGNOSTIC STUDY OF HEMOPLASMOSIS IN CATS IN BASRAH CITY-IRAQ Texto completo
2020
Sajjad L. Jabbar | Mohammed A.Y. Al. Amery
The cats might severely affected and became carrier for Hemoplasma spp., sothe current study were aimed to investigate hemoplasmosis in cate in Basarh city,Iraq.This work was conducted via examine (20) cats of local cat breeds in Basarhcity, using their blood samples for stained smear and blood parameters.The Giemsastained blood smears revealed seven (35%) cats infected with Hemoplasmosis.However, thirteen (65%) was found negative. Diseased cats show pale and or/ictericmucos membranes, dehydration, emaciation, loos of appetite, and weakness,Moreover , a significant increase of body temperature, respiratory and heart rate wasindicated. Results was also show a significant decrease of RBC,HB and PCV ofdiseased cats which reflected Normocytic Normochromic type of anemia.Hemoplasma spp. of infected cats appears round or rod shape singular or chainedlocated on the cell membranes of the infected erythrocytes .It was concluded thatfeline mycoplasmosis lead to deleterious effects which might terminated with deathof affected cats .
Mostrar más [+] Menos [-]Screening of the antiparasitic activity of gold nanoparticles on hydatid cysts protoscolices in vitro Texto completo
2023
Anah, S.A.
Many scolicidal agents have been used to destroy fertile protoscolices, but these scolicidal agents have side effects, highlighting the need for research on effective and non-toxic replacement scolicidal agents. Gold nanoparticles (AuNPs) are biocompatible and non-toxic. The current study examined the effects of AuNPs in killing the protoscolices of Echinococcus granulosus in vitro using eosin staining. The protoscolices were treated with 0.2, 0.4, 0.8, or 1.0 mg/mL of AuNPs for 15, 30, 45, or 60 minutes. A concentration of 1.0 mg/mL was the most efficient in killing the protoscolices after 60 minutes exposure, reaching 96%, followed by 0.8 mg/mL (84.5%), whereas 0.4 and 0.2 mg/mL of AuNPs achieved a death rate of 76.8% and 68.5%, respectively. The loss of the protoscolices was lower at shorter exposure times with the same concentration of AuNPs and increased as the AuNP concentration was increased at the same exposure time. Significant differences were found between the different groups compared to the control group.
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