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Control of canine respiratory and diarrheal disease using egg yolk antibodies I. Induction of antibody in hens immunized with combined antigens of Bordetella bronchiseptica, parvovirus and canine distempervirus
Lee, H.S.;Kim, J.M.;Woo, S.R.;Jung, B.Y.;Cho, Y.S.;Tark, D.S.;Lim, S.K.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)E-mail:leehsoo@nvrqs.go.kr | Yoo, H.S.(Seoul National University, Seoul, Republic of Korea) | Yoon, Y.D.;Oh, J.S.(Green Cross Veterinary Science Institute, Yongin, Republic of Korea) | Huh, W.;Mun, Y.S.(Dae Sung Microbiology Laboratory, Uiwang, Republic of Korea)
This study was carried out to produce IgY against B. bronchiseptica, parvovirus and distemper virus that are major pathogens in alimentary and/or respiratory diseases of dogs. In the comparison of adjuvants, ISA70 was the best in the rapid induction and maintence of antibody titers. Agglutination antibody titers against B. bronchiseptica were 1:1,280~1:10,240 in sera and 1:160~1:1,280 in egg yolk. Hemagglutination inhibition(HI) titers against parvovirus in sera and egg yolk were 1:80~1:320 and 1:64~1:256, respectively. Virus neutralization titers against canine distemper was 1:8~1:64 in sera and egg yolk.
Mostrar más [+] Menos [-]Control of canine respiratory and diarrheal disease using egg yolk antibodies II. Immunoprophylatic effect of egg yolk antibodies in mice and dogs
Lee, H.S.;Kim, J.M.;Woo, S.R.;Jung, B.Y.;Cho, Y.S.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)E-mail:leehsoo@nvrqs.go.kr | Yoo, H.S.(Seoul National University, Seoul, Republic of Korea) | Yoon, Y.D.;Oh, J.S.(Green Cross Veterinary Science Institute, Yongin, Republic of Korea) | Huh, W.;Mun, Y.S.(Dae Sung Microbiology Laboratory, Euiwang, Republic of Korea)
Immunoprophylatic effect of IgY against B. bronchispetica was proven with 100% preventive rate in mice administrated with IgY with antibody titer 1:640~1:2,560. Intramuscular administration was more efficient than oral administration. This phenomenon was also observed in the therapeutic effects of IgY after challenge with B. bronchseptica in mice. In the field trials with the egg yolk antibodies from hens immunized with combined antigens with B. bronchiseptica and parvovirus, curing rates in dogs with severe clnical signs such as bloody diarrhea were 81.6% and 86.7% by intramuscular or subcutaneous administration of IgY, respectively.
Mostrar más [+] Menos [-]Yolk sac utilization in ostrich (Struthio camelus) chicks
2004
Mushi, E.Z. (Botswana Agriculture College, Gaborone (Botswana)) | Binta, M.G. | Chabo, R.G.
The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus
1999
Du Plessis, D.H. | Van Wyngaardt, W. | Romito, M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Du Plessis, M. | Maree, S.
