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Reclassification of North American leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis.
1986
Thiermann A.B. | Handsaker A.L. | Foley J.W. | White F.H. | Kingscote B.F.
Antibody response to genus- and serovar-specific leptospiral antigens in Leptospira-infected cows.
1985
Fairbrother J.M.
Evaluation of safety and immunogenicity of a new octavalent inactivated vaccine containing porcine parvovirus, erysipelas, and leptospira
2017
Kim, K., Kangwon National University, Chuncheon, Republic of Korea | Choi, J.Y., Kangwon National University, Chuncheon, Republic of Korea | Park, S.J., Zoetis Korea, Seoul, Republic of Korea | Hahn, T.W., Kangwon National University, Chuncheon, Republic of Korea
Porcine parvovirus, Erysipelothrix (E.) rhusiopathiae, and Leptospira (L.) interrogans are considered major etiologic agents of reproductive failure in pigs, causing economic loss in the swine industry. In this study, the safety and immunogenicity of a new octavalent inactivated vaccine were evaluated. The vaccine contained inactivated porcine parvovirus, E. rhusiopathiae, and six L. interrogans serovars (Bratislava, Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, and Pomona). Safety test results showed no notable side effects or clinical signs after vaccination in mice, guinea pigs, and sows. In addition, we assessed immunogenicity of the vaccine in 25 sows under field conditions. The vaccinated group (n = 20) had a significantly higher antibody level than the non-vaccinated group (n = 5). Moreover, the stillbirth rate decreased in piglets born from vaccinated sows, resulting in an increased fertility rate. The results of this study demonstrate that the new octavalent inactivated vaccine can be applied safely and effectively to improve reproductive performance in sows.
Mostrar más [+] Menos [-]Comparison of polymerase chain reaction assays with bacteriologic culture, immunofluorescence, and nucleic acid hybridization for detection of Leptospira borgpetersenii serovar hardjo in urine of cattle
2000
Wagenaar, J. | Zuerner, R.L. | Alt, D. | Bolin, C.A.
Latex agglutination test based on the recombinant outer membrane proteins for serodiagnosis of leptospirosis in goats.
2011
Chandra, Anjul | Srivastava, S. K. | Chaudhuri, P. | Prakash, M .M.
A total of 281 serum samples collected randomly from goats showing the signs of fever, abortion,repeat breeding and still births as well as from apparently healthy ones were subjected to LAT and MAT based on rLipL32 and rLipL41 antigens. A total of 16 (5.69%) samples were found positive to MAT, whereas rLipL32-LAT and rLipL41-LAT detected 35 (12.45%) and 23 (8.18%) samples as positive, respectively. The sensitivity and specificity of rLipL32-LAT was 87.50% and 92.83%, respectively,while rLipL41-LAT yielded 75.00% and 97.35% sensitivity and specificity, respectively. LAT based on rLipL32 and rLipL41antigens could further be evaluated on a larger number of samples to ensure its utility as a screening test for the sero-epidemiological studies.
Mostrar más [+] Menos [-]Evaluation of recombinant LipL32 and LipL41 antigens of Leptospira interrogans serovar Canicola by ELISA for serodiagnosis of bovine leptospirosis.
2010
Sankar, Surya | Chaudhury, Pallab | Verma, Rishendra | Harshan, Hiron .M. | Srivastava, S.K.
Recombinant LipL32 and LipL41 outer membrane proteins of Leptospira interrogans serovar Canicola were produced, and used as a pooled antigen in enzyme-linked immunosorbent assay (ELISA) to detect leptospiral antibodies in bovine sera samples. The optimum concentration of the pooled antigen was found to be 50ng of each antigen per well by using known positive and negative cattle sera. Using a total of 500 bovine sera samples the sensitivity, specificity and accuracy of pooled antigen based ELISA as compared to microscopic agglutination test (MAT) were 100%, 88.1% and 91.6%, respectively. The results suggested that antigen in ELISA could be preferred for detection of all those cases, which might have remained undiagnosed by performing MAT.
Mostrar más [+] Menos [-]Seroprevalence of bovine leptospirosis in Garanhuns municipal district, Pernambuco State, Brazil
2001
Oliveira, A.A.F. | Mota, R.A. | Pereira, G.C. (Pernambuco Federal Rural Univ., Dois Irmaos (Brazil). Veterinary Medicine Dept.) | Langoni, H. | Souza, M.I. | Navegantes, W.A. | Sa, M.E.P.
Serological prevalence of leptospiral antibodies in pigs in South Africa
1995
Potts, A.D. | Loetter, C. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Robinson, J.T.R.
Serological survey on the leptospiral antibody in domestic animals in the area where human leptospirosis occurred
1991
Seo, I.S. (Seoul National Univ., Suwon (Korea Republic). Coll. of Veterinary Medicine)
Molecular detection of leptospiral carriers in sheep under tropical field conditions | Detecção molecular de ovinos carreadores de Leptospira em ambiente tropical Texto completo
2014
Ariel Director | Gabriel Mendes de Souza Martins | Ana Paula Pereira Loureiro | Camila Hamond Regua Motta Reis | Marco Alberto Medeiros | Walter Lilenbaum
Molecular detection of leptospiral carriers in sheep under tropical field conditions | Detecção molecular de ovinos carreadores de Leptospira em ambiente tropical Texto completo
2014
Ariel Director | Gabriel Mendes de Souza Martins | Ana Paula Pereira Loureiro | Camila Hamond Regua Motta Reis | Marco Alberto Medeiros | Walter Lilenbaum
O objetivo do presente estudo foi analisar a aplicabilidade da PCR na detecção de ovinos carreadores de <em>Leptospira</em> em ambiente tropical. Brevemente, dois rebanhos ovinos, previamente reportados como sororeativo (A) e soronegativo (B) foram selecionados para este estudo. Da totalidade de animais de cada rebanho, amostras de urina e fluido vaginal (FV)/sêmen foram colhidas para cultura bacteriológica e PCR. Além disso, amostras de soro foram colhidas e utilizadas na sorologia (teste da soroaglutinação microscópica). Esta técnica confirmou o estado prévio dos dois rebanhos. Nenhuma amostra pura de leptospiras foi obtida no cultivo. Já na PCR, animais do Rebanho A apresentaram 26,7% (FV), 33,3% (sêmen) e 38,9% (urina) de amostras positivas. O Rebanho B apresentou 40,0% (FV), 33,3% (sêmen) e 5,6% (urina) de positividade pela PCR. Em conclusão, a PCR foi uma importante ferramenta na identificação de carreadores de leptospiras, incluindo animais do rebanho soronegativo, o que reforça as vantagens do uso desta técnica para a detecção de ovinos portadores como parte dos programas de controle da leptospirose em ambiente tropical. | The purpose of this study was to analyze the usefulness of PCR for the detection of leptospiral carriers in sheep under tropical field conditions. Two flocks, previously reported as seroreactive (A) and seronegative (B), were selected for this study. From those, the totality of animals of each flock, urine and vaginal fluid (VF)/semen were collected for bacteriological culture and PCR, as well as serum samples for serology. Serology confirmed the previous status of the two flocks. Culture was negative for all the samples. In PCR, animals of Flock A presented 26.7% (VF), 33.3% (semen) and 38.9% (urine) of positivity. Flock B presented 40.0% (VF), 33.3% (semen) and 5.6% (urine) of positivity by PCR. In conclusion, PCR was important to identify carriers of leptospires, including animals from a seronegative flock, what reinforces the advantages of the usage of this tool for the detection of carriers in sheep as part of control programs of leptospirosis under tropical field conditions.<strong></strong>
Mostrar más [+] Menos [-]Molecular detection of leptospiral carriers in sheep under tropical field conditions Texto completo
2014
Ariel Director | Gabriel Mendes de Souza Martins | Ana Paula Pereira Loureiro | Camila Hamond Regua Motta Reis | Marco Alberto Medeiros | Walter Lilenbaum
The purpose of this study was to analyze the usefulness of PCR for the detection of leptospiral carriers in sheep under tropical field conditions. Two flocks, previously reported as seroreactive (A) and seronegative (B), were selected for this study. From those, the totality of animals of each flock, urine and vaginal fluid (VF)/semen were collected for bacteriological culture and PCR, as well as serum samples for serology. Serology confirmed the previous status of the two flocks. Culture was negative for all the samples. In PCR, animals of Flock A presented 26.7% (VF), 33.3% (semen) and 38.9% (urine) of positivity. Flock B presented 40.0% (VF), 33.3% (semen) and 5.6% (urine) of positivity by PCR. In conclusion, PCR was important to identify carriers of leptospires, including animals from a seronegative flock, what reinforces the advantages of the usage of this tool for the detection of carriers in sheep as part of control programs of leptospirosis under tropical field conditions.
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