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Modulation of thermal killing of bovine lymphocytes and preimplantation mouse embryos by alanine and taurine.
1992
Malayer J.R. | Pollard J.W. | Hansen P.J.
Addition of alanine and taurine blocked killing of lymphocytes caused by culture at 45 C. The optimal concentration for thermoprotection was achieved at 12.5 mM for L-alanine and 5 mM for taurine. Both D and L forms of alanine provided thermoprotection. The effect of these agents was not simply to increase osmolarity of the culture medium, because NaCl did not provide thermoprotection at comparable concentrations. Alanine and taurine were each tested at concentration of 50 mM for ability to block heat shock-induced killing and developmental retardation of 8- to 16-cell mouse embryos. Both agents enhanced embryo development after exposure to high temperature, though development remained less than that for embryos not exposed to high temperature. In one experiment, for example, 81% of embryos cultured at 38 C advanced in development during culture vs 0% at 42 C, 15% at 42 C with alanine, and 32% at 42 C with taurine. The beneficial effect of alanine at high temperature may have been partly attributable to effects independent of thermoprotection, because development of embryos cultured at 38 C was also improved by alanine.
Mostrar más [+] Menos [-]Isolation and morphological characterization of natural killer cell in the Sprague-dawley (SD) rats.
1992
Kang K.S. | Lee Y.S.
Immune response to Echinococcus multilocularis infection in the mouse model: A review
1992
Playford, M.C. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Kamiya, M.
Immunotoxic effects of Vomitoxin
1992
Kim, J.S. | Kim, Y.H. (Gyeongsang Nat'l Univ., Chinju (Korea Republic). Coll. of Veterinary Medicine)
Comparison of sheep erythrocytes and Korean native goat erythrocytes-rosette forming rate of pig peripheral blood mononuclear cells
1992
Kim, Y.J. | Song, H.J. | Kim, J.M. | Kang, M.D. | Yoon, C.Y. | Kim, T.J. (Chonbuk Nat'l Univ., Chonju (Korea Republic). Coll. of Veterinary Medicine)
Studies on the contact infection of canine demodicosis (1)
1992
Lee, C.Y. | Lee, C.H. | Seo, G.W. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine)
