Maggots are one of the fly larvae with a high protein content of up to 30-45%, so maggots can be used as an alternative animal feed ingredient. As one of the sources of animal feed raw materials, insects-based feed must also be safe from contaminants of microorganisms, including pathogenic bacteria. Therefore, this study aims to isolate and determine the screening of pathogenic and non-pathogenic bacteria from maggots' digestive systems. Maggots are cultivated on media based on animal faecal and organic waste for 2 months. Then, the bacteria are isolated and purified using nutrient agar media, and hemolysis tests are carried out on blood agar media and Gram staining. Eighty-seven bacterial colonies with various morphological characteristics were successfully isolated from the maggot digestive system. Most isolated bacteria are classified as Gram-negative bacteria with a bacilli form. Based on the results of the hemolysis test, as many as 16% of bacterial isolates are indicated by pathogenic bacteria because of their ability to hemolyze blood. However, only about 2% showed b-hemolysis. Thus, it can be concluded that the screening results of non-pathogenic bacteria are still more numerous compared to pathogenic bacteria present in the maggot digestive system.

The study was carried out to investigate the associated maggots and bacteria in myiasis affected wound in animals. Total 37 myiasis affected cattle (n=25) and goats (n=12) were examined in this study, of which 21 samples were collected from the Veterinary clinics of Bangladesh Agricultural University (BAU), Mymensingh, and the remaining 16 samples were collected from Upazila Veterinary Hospital, Gouripur, Mymensingh. The maggots were collected from the wounds, and were identified under microscope. For microbial identification, samples (n=10) were collected aseptically by using sterile cotton swab. The samples were allowed to grow in media. Colony and staining characteristics were studied. Traumatic, creeping or migratory myiasis wounds were identified in the examined animals. In this study, subcutaneous wound was mostly recorded, of which 43% (n=9/21) was found at the Veterinary Clinics, BAU, and 44% (n=7/16) was found at the Upazila Veterinary Hospital, Gouripur. The maggots were identified as Chrysomya bezziana, commonly known as Old World screwworm. Occurrence of myiasis due to C. bezziana larvae was recorded as 100%. The associated bacteria were identified as Staphylococcus spp. In conclusion, the myiasis wounds are associated with both Staphylococcus spp. and larvae of C. bezziana. Preventive and control measures against the C. bezziana and Staphylococcus spp. can be undertaken to save the livestock from myiasis in Bangladesh.