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The effects of mixed infection of strongyles in experimental animals in the Veterinary Research Institute
2014
Chong, K. L. | Ng, Agnes W. S. | Ramlan M. | Zaini C. M. | Premaalatha B. | Chandrawathani P. | Lily Rozita M. H. | Erwanas A. I. | Lee, C. H.
This paper reports the relationship between mixed infections ofstrongyles with fecal egg count (FEC), packed cell volume (PCV) of the blood and FAMACHA in experimental sheep in VRI. The third larval stage (L3) of strongylid nematode were fed orally to two experimental sheep. Each animal was given 3 ml L3 or equivalent to 3000 larvae. Faecal samples were then collected weekly for a 10 week duration. The FAMACHA scale for anemia estimation was used and blood collection through ear tip was also done twice a week to measure the packed cell volume (PCV) of the blood. The results show that the PCV decreases while faecal egg counts increase over the study period.The FAMACHA scale also increases with time indicating a progressive anemia with the decreasing PCV. As helminth infections are common in small ruminants, the effects of anemia are critical parameters to be monitored in any flock as this will determine its productivity in terms of mortality and morbidity.
Mostrar más [+] Menos [-]Evaluation on the reproductive performance and spontaneous malformations amongst SD rats in the institute for medical research colony
2014
Hasnan J. | Siti Amirah S. | Paumgartten, F.J.R. | Hussin M. | Zakiah I.
This study was performed to investigate the reproductive performanceand spontaneous malformations of female Sprague Dawley (SD) rats obtained from the Animal Resource Unit, Institute for Medical Research for their use in reproductive toxicity assessment. Onehundred and thirty-five virgin female rats with a body weight ranging between 190 g and 210 g were randomly caged overnight with 60 fertile male rats in 1:1 basis. Vaginal smear was performed the next morning and the day of sperm positive was considered as estational day (GD) 0. On GD21 caesarean hysterectomy was performed to examine the outcome of each pregnancy. The female fertility index (sperm positive) was 90.5% and the pregnancy index was 100%. Thepercentage of pre and post-implantation loss were 1.96 and 7.48 respectively with the number of early resorption out numbered the late resorption (0.56 ± 1.0 and 0.36 ± 1.5 respectively). Out of1,234 foetuses examined, only 5 foetuses (0.41%) presented with gross congenital malformation. The female SD rats obtained from the Institute demonstrated high fertility and pregnancy indexes with lowincidence of resorption and malformation, therefore suitable to use for reproductive toxicity assessment.
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