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Фенотипические особенности ремонтных телочек, полученных методом трансплантации замороженно-оттаянных эмбрионов
2009
Minina, N.G., Grodno State Agrarian Univ. (Belarus)
The study has stated that heifer replacements, from the date of birth to 18 months, got through transplantation of frozen-defrosted embryos were not inferior to and in many cases superior to the heifers of their age but got through traditional reproduction. Transplant first-calf heifers have a high level of milk productivity which amounts to 7536,10 kilos, with 3,62% of fat and 3,23% of protein. Thus they provide a good selection material for new generation champions to be chosen among them and for being used as mothers of bulls for service
Mostrar más [+] Menos [-]Использование биологически активных веществ при созревании ооцитов крупного рогатого скота вне организма
2009
Rakovich, E.D., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
The problem of creation of improved conditions for cattle oocyte maturation in vitro was studied in the Republic of Belarus. The objects of study were cattle oocytes and embryos of early preimplantation stages of development obtained in vitro conditions. Research results showed that application of bovine serum in complex with embryonic or fetal serum in the process of oocyte cultivation in vitro conditions made it possible to obtain 35,5-44,8% of breaking down cells and 16,1-17,2 % of embryos at the stage of morula-blastocyte. Entering of 0,02 ng/ml of gonadotropin-releasing hormone (Surfagon) into media for oocyte maturation at the cost of regulation of the processes of growing and development of oocyte-cumulus complexes made it possible to increase of yield of matured till the stage of metaphase II oocytes up to 89,3 %. At the same time, the yield of divided embryos after fertilization increased on 2,5 % in comparison with control indexes and amounted 45,2 %. Research results showed that the synthetic phytohormone epibrassinolide could be used as a biologically active factor in the process of getting early embryos in vitro in concentration of 2 x 10E-7 – 2 x 10E-9 mole/l. It made it possible to get 46,4-55,0 % of divided cells and 14,2-16,2% preimplantation embryos
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