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Pathogenicity of Yersinia Ruckeri in Rainbow Trout (Oncorhynchus Mykiss)
2018
Taheri mirghaed, ali | Soltani, mehdi | Shafiei, Shafigh | Mirzargar, Seyed saeed | Shokrpur, Sara
BACKGROUND: Yersiniosis or enteric redmouth disease (ERM), caused by Yersinia ruckeri, is a serious bacterial disease in the farmed salmonids that causes economic problems in this industry. OBJECTIVES: This study was aimed to assess the experimental pathogenicity of Yersinia ruckeri in Rainbow trout (Oncorhynchus mykiss). METHODS: Two hundred Rainbow trout weighting 100-120 g, challenged with different strain of Yersinia ruckeri were obtained from affected trout farms using intra peritoneal injection route at a concentration of 108 cells/ml (0.1 mL per fish) to evaluate the virulence of these isolates. Each treatment group included 10 fish in two replicates and control fish received 0.1 mL sterile normal saline (0.9% NaCl). Following the intra peritoneal challenge, macroscopic and microscopic findings were determined. The most virulent strain was then used to determine the lethal concentration (LD50) using both intra peritoneal and bath method at dilutions of 103-1010 cells/mL. RESULTS: Macroscopically, anorexia, lethargy, circular swimming near the surface, blackening of skin, exophthalmia, hyperemia and hemorrhage in different parts of body, anal prolapse, enlarged liver and spleen were observed. Microscopically, hyperemia of hepatic sinusoids and vessels, necrosis and vacuolization of hepatocytes, increase in the abundance of macrophage centers in kidney, dilatation of Bowman’s space, degeneration and necrosis of kidney tubules, severe necrosis and detachment of intestinal villi, hyperplasia and clubbing of epithelial cells of secondary lamellae, spleen cell necrosis, goblet cell hyperplasia and thickening of epidermis layer in the tongue mucosa were observed. The LD50 of intra peritoneal injection was calculated 1.2×106cells per fish 48 h post challenge. In bath route, LD50 was obtained 5×108 Cells/ml after 96 h. CONCLUSIONS: The results obtained from this study show virulence diversity of native strains.
Mostrar más [+] Menos [-]Pathogenicity of porcine enterotoxigenic Escherichia coli that do not express K88, K99, F41, or 987P adhesins.
1992
Casey T.A. | Nagy B. | Moon H.W.
Three-week-old weaned and colostrum-deprived neonatal (< 1 day old) pigs were inoculated to determine the pathogenicity of 2 enterotoxigenic Escherichia coli isolates that do not express K88, K99, F41, or 987P adhesins (strains 2134 and 2171). Strains 2134 and 2171 were isolated from pigs that had diarrhea after weaning attributable to enterotoxigenic E coli infection. We found that both strains of E coli adhered in the ileum and caused diarrhea in pigs of both age groups. In control experiments, adherent bacteria were not seen in the ileum of pigs < 1 day old or 3 weeks old that were noninoculated or inoculated with a nonpathogenic strain of E coli. These control pigs did not develop diarrhea. Antisera raised against strains 2134 and 2171 and absorbed with the autologous strain, grown at 18 C, were used for bacterial-agglutination and colony-immunoblot assays. Both absorbed antisera reacted with strains 2134 and 2171, but not with strains that express K99, F41, or 987P adhesins. A cross-reaction was observed with 2 wild-type K88 strains, but not with a K12 strain that expresses K88 pili. Indirect immunofluorescence with these absorbed antisera revealed adherent bacteria in frozen sections of ileum from pigs infected with either strain. We concluded that these strains are pathogenic and express a common surface antigen that may be a novel adhesin in E coli strains that cause diarrhea in weaned pigs.
Mostrar más [+] Menos [-]Inoculation of pigs with Streptococcus suis type 2 alone or in combination with pseudorabies virus.
1992
Iglesias J.G. | Trujano M. | Xu J.
Pigs [9+/-1] weeks old) were inoculated with Streptococcus suis type 2, pseudorabies virus (PRV) or both. For each pig of groups A, B, and C the inoculum of S suis was 10(9) colony-forming units. For each pig of groups A, B, and D the inoculum of PRV was 5 X 10(3) TCID50 of either PRV strain 4892 (group A, n = 9) or PRV isolate B (group B, n = 9). The PRV strain 4892 is a highly virulent strain; isolate B causes mild clinical signs of infection in inoculated pigs. Group-C pigs (n = 9) were given S suis alone, and group-D pigs (n = 3) were inoculated only with PRV isolate B. Clinical signs of infection and development of lesions were readily seen in pigs of groups A, B, and C. Duration and severity of clinical signs of disease and lesions were reduced in pigs of group C, compared with those of the other 2 groups. Lesions, such as polyarthritis and fibrinous pericarditis, were more abundant and acute in the groups of pigs given mixed challenge exposure, compared with pigs inoculated exclusively with S suis type 2 (group C). The group of pigs inoculated with PRV isolate B alone did not manifest clinical signs of disease or lesions. Average daily gain for group-C pigs was higher, compared with that of other groups; the difference was statistically significant at P < 0.02 and P < 0.05 for groups B and D, respectively. Spread of S suis within the tissues of infected pigs was higher in pigs of groups A and B, compared with pigs of group C. Total number of isolations was 8, 15, and 7 for groups A, B, and C, respectively; S suis was isolated from more than 1 tissue specimen from some pigs. The rate of pigs carrying S suis was 4 of 4 in group-A, 7 of 9 in group-B, and 5 of 9 in group-C pigs. It was concluded that clinical disease associated with S suis type 2 was enhanced by concomitant infection with PRV and such effect was common to both PRV strains tested, the highly virulent strain and the strain with low virulence.
Mostrar más [+] Menos [-]Effect of Pasteurella haemolytica-derived endotoxin on pulmonary structure and function in calves.
1990
Slocombe R.F. | Mulks M. | Killingsworth C.R. | Derksen F.J. | Robinson N.E.
The role of endotoxin in the pathogenesis of acute pneumonic pasteurellosis is uncertain. Recently, we reported that Escherichia coli-derived endotoxin given by airway inoculation fails to induce lung injury in calves. Because Pasteurella haemolytica-derived endotoxin may differ substantially from E coli in its pathogenicity, we repeated these studies with Pasteurella endotoxin. Intratracheal inoculation of P haemolytica endotoxin caused hypoxemia and increased the alveolar-arterial oxygen differences without causing hypercarbia or changes in lung mechanical properties and volumes. In contrast, IV inoculation of endotoxin caused systemic hypotension, leukopenia, gas exchange impairment, increased total pulmonary resistance, and decreased dynamic compliance. Both routes of inoculation increased serum endotoxin concentrations and were associated with areas of pulmonary hemorrhage, edema, and acute inflammation. We concluded that P haemolytica-derived endotoxin is pathogenic by IV and airway routes of inoculation, and therefore differs from E coli endotoxin in its ability to induce lung lesions in calves.
Mostrar más [+] Menos [-]Histopathologic changes in bovine fetuses after repeated reintroduction of a spirochete-like agent into pregnant heifers: association with epizootic bovine abortion.
1987
Osebold J.W. | Osburn B.I. | Spezialetti R. | Bushnell R.B. | Stott J.L.
Linkage of serum resistance, aerobactin production, and resistance to antimicrobial agents on conjugal plasmids in some strains of Escherichia coli isolated from septic foals.
1993
Hirsh D.C. | Kirkham C. | Wilson W.D.
Fifteen isolates of Escherichia coli obtained from the blood and tissues of septic foals had plasmid DNA of size ranging from 2.5 to 93 megadaltons. These isolates grew in normal equine serum (serum resistant), a trait previously documented to be expressed by isolates obtained from blood and tissues of septic foals, but not by isolates obtained from the feces of clinically normal horses. Of these isolates, 3 contained conjugal plasmids that encoded resistance to multiple antimicrobial agents linked to serum resistance and, in 1 isolate, to production of aerobactin as well. Serum resistance and production of aerobactin are related to virulence of septicemic E coli from non-equine sources.
Mostrar más [+] Menos [-]Comparison of phenotypic characteristics of Salmonella spp isolated from healthy and ill (infected) chickens.
1991
Nolan L.K. | Wooley R.E. | Brown J. | Payeur J.B.
Phenotypic characteristics of 12 paired, Salmonella serotypes isolated from healthy and ill chickens were compared. Variables compared included antibiotic resistance profiles, production of colicins and siderophores, mannose-sensitive hemagglutination of erythrocytes, resistance to serum complement, carbon source utilization, presence and transmissibility of R plasmids, and invasiveness in primary chicken kidney cell culture. Differences were found between pairs for utilization of carbon sources, mannose-sensitive hemagglutination of erythrocytes, and invasiveness in cell culture.
Mostrar más [+] Menos [-]Studies on the pathogenesis of Salmonella heidelberg infection in weanling pigs.
1985
Reed W.M. | Olander H.J. | Thacker H.L.
Studies on pathogenicity of Escherichia coli isolated from chickens: Correlation between in vitro Congo-red binding properties and in vivo virulence in avian Escherichia coli.
1991
Woo Y.K. | Kim K.S. | Kim B.H.
Pathogenicity, hemagglutinability and the effect of physicochemical agents on virus of rabbit hemorrhagic disease.
1990
Yoon I.J. | Jeon Y.S.