Refinar búsqueda
Resultados 1-6 de 6
Evaluation of the specificity of Pasteurella multocida somatic antigen-typing antisera prepared in chickens, using ribosome-lipopolysaccharide complexes as inocula.
1989
Rimler R.B. | Angus R.D. | Phillips M.
Purified lipopolysaccharides (LPS) from 16 serotypes of Pasteurella multocida were complexed with Aspergillus fumigatus ribosomes. The complexes were used as inocula to prepare antisera, in chickens, for somatic antigen typing by the gel diffusion precipitin test (GDPT). Antisera made against 15 of 16 LPS reacted with their respective specific heat-stable antigens in the GDPT and homologous LPS in the passive hemagglutination test. Antisera could not be made against serotype 15 LPS. Correlation was not observed between intensity of the precipitin reaction in the GDPT and titer to homologous LPS in the passive hemagglutination test. Most antisera cross-related with other heat-stable antigens of other serotypes in the GDPT. Many of these cross-reactions were eliminated by dilution. Cross-reactions that occurred in the GDPT with antisera made against LPS of serotypes 2, 5, 7 and 8 could not be eliminated by dilution.
Mostrar más [+] Menos [-]Distribution of thermophilic Camphylobacters in animals and transfer of drug resistance factor of isolates to related bacteria., 1; Distribution and drug resistance of thermophilic Campylobacters isolated from animals
1989
Kim, Y.H. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Veterinary Medicine) | Mah, J.S. (Seoul National Univ., Suwon (Korea R.). Coll. of Veterinary Medicine)
To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campylobacter spp., isolation of etiological agent was carried out. Isolated Campylobacter spp. were biotyped, serotyped and the susceptibility of the isolates to antimicrobial agents were examined. Isolation rates of Campylobacter spp. from 649 fecal materials of 208 cattle, 300 pigs and 141 chickens were 25.5 %, 23.7 % and 38.3 %, respectively. The majority of the 130 isolates of C jejuni was classified as biotype I (50.6 %) and biotype II (34.6 %). Most of the 46 isolates of C coli were biotype I (71.7 %). Isolated C jejuni strains showed 14 different serotype, and serotype 4, 26, 36 were most frequent. Isolated C coli strains showed 5 different serotype and serotype 31 and 21 were relatively common. Isolated Campylobacter spp. were highly susceptible to nalidixic acid, amikacin, gentamycin, colistin and chlorampehnicol
Mostrar más [+] Menos [-]Mucormycosis in a group of chickens
1989
Cheong, K.S. (Yongdong Branch of Veterinary Research Inst., (Korea R.)) | Rim, B.M. (Chonbug National Univ., Chonju (Korea R.). Coll. of Veterinary Medicine)
The etiologic agents of mucormycosis are several nonpathogenic fungi of the order Mucorales and are ubiquitous. A total of 82 chickens were infected with the disease and developed anorexia, diarrhea, malnutrition, dyspnea, and paralysis in a chicken farm, Sokcho, Kwangwon. At necropsy there were multiple nodular lesions and hemorrhages in livers, spleens, kidneys, gastro intestinal track, and respiratory system. On histopathological examination it was found that the nodular lesions were consisted of granulomatous inflammation accompanying characteristic hyphae (4-24 micro wide) of the fungi
Mostrar más [+] Menos [-]Distribution of thermophilic Campylobacters in animals and transfer of drug resistance factor of isolates to related bacteria., 2; Plasmid profile and transfer of drug resistance of isolated Campylobacter
1989
Kim, Y.H. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Veterinary Medicine) | Mah, J.S. (Seoul National Univ., Suwon (Korea R.). Coll. of Veterinary Medicine)
To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campylobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Camplyobacter spp. were examined. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60 % of the isolates were noted to harbor plasmid DNA. Plasmid occurrence rate from pigs, chickens and cattle were 76.2 %, 61.7 % and 37.7 %, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10-1,000. Tetracycline resistant plasmids of C jejuni were transferarble to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E. coli and Vibrio cholerae. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation
Mostrar más [+] Menos [-]Suceptibility to animal serum and antimicrobial agents of campylobacter jejuni isolated from pigs and chicken
1989
Lee, S.C. | Kang, H.J. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Veteinary Medicine)
A total of 108 strains of C jejuni isolated from pigs and chickens were examined for the susceptibility to 10 antimicrobial agents and normal sera of cattle, sheep, guinea pigs and chickens. Minimal inhibitory concentration (MIC) ranges of antimicrobial agents to C jejuni isolates were = 100 micro g/ml for erythromycin, rifamycin, streptomycin and tetracycline, 50 to 100 micro g for cephalothin, = 50 micro g for ampicillin,
Mostrar más [+] Menos [-]Studies on avian infectious bronchitis: II. Standardization of an indirect enzyme-linked immunosorbent assay (ELISA) for antibody measurement
1989
Chang, C.H. | Kim, S.J. (Seoul National Univ., Suwon (Korea R.). Coll. of Veterinary Medicine)
Critical parameters affecting sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to avain infections bronchitis virus (IBV) were standardied. By adopting the optimized conditions an equation calculating ELISA antibody titers from the observations at single serum dilution was formulated. The purified antigen of IBV lMass-41 strain was dispensed into polystyrene microplate wells at a concentration of 300ng per well (100 micr l) and the plates were coated by completey drying at 37deg C. Diluted chicken serum and horseradish peroxidase conjugated goat anti-chicken IgG were added in order in 100 micro l volumes per well and allowed to react for 30 minutes each at room temperature. Just before use and after each reaction the plates were washed three times with distilled water. Finally o-phenylenediamine solution was added as an enzyme substrate. After incubation for another 15 minutes at room temperature absorbances were read at 492 nm. Hyperimmune serum against Mass-41 strain was used as internal reference positive (IRP)serum. After repeated titration of IRP and negative sera, a constant titer of IRP was determined. Serum titrations were carried out for various sample sera together with IRP and negative sera and the observed titers of sample sera were corrected by reflecting the ratio between observed and constant titers of IRP serum. These corrected titers of the sample sera were plotted against sample/positive (S/P)OD ratios. All the OD's measured in the serum titrations were also corrected by substracting negative serum OD. The following equation was formulated from the above data; Log10 ELISA titer = 5.568 (log10 S/P) + 4.161. Thus it was possible to calculate ELISA titer by measuring absorbance at 1/400 single serum dilution. Titer measured by cross ELISA tests employing Mass-41 strain and three local IBV isolates were similar. These results suggest that the ELISA tests standardized in this study can be used for evaluating not only vaccinal immunity but also for infection status against fields IBV's
Mostrar más [+] Menos [-]