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Taxonomic justification of the pathogenic strongylid infecting the Arabian camel Camelus dromedarius as Haemonchus longistipes by morphological and molecular phylogeny Texto completo
2019
Dajem, Saad | Morsy, Kareem | Al-Kahtani, Mohammed | Abdel-Gaber, Rewaida
Taxonomic justification of the pathogenic strongylid infecting the Arabian camel Camelus dromedarius as Haemonchus longistipes by morphological and molecular phylogeny Texto completo
2019
Dajem, Saad | Morsy, Kareem | Al-Kahtani, Mohammed | Abdel-Gaber, Rewaida
Introduction: There is still lack of morphological and phylogenetic information on the pathogenic nematode of the camel Haemonchus longistipes. In the present study, this parasite was isolated in Saudi Arabia and described. Material and Methods: The abomasa of two Arabian camels were collected from a slaughterhouse in Abha province and examined for nematode infection. Worms were described morphologically and morphometrically by electron microscopy. Multiple sequence alignment and the phylogenetic tree of the parasite were constructed from maximum likelihood analysis of its ITS-2 rDNA sequences. Results: These nematodes had a slender body terminating anteriorly at a conspicuous dorsal lancet. A pair of lateral cervical papillae distant from the anterior end was observed. The buccal aperture was hexagonal and surrounded by two amphids, six externo-labial papillae, and four cephalic papillae. Males terminated posteriorly at a bursa supported by spicules and lateral and dorsal rays. Females were linguiform and knobbed morphotypes with distinct ovijectors and a dorsal rim covering the anal pore. The taxonomy was confirmed by the morphology and number of the longitudinal cuticular ridges in a 43–46 range. The sequence alignment and phylogeny revealed 92% homology with H. longistipes (AJ577461.1), and the sequence was deposited into GenBank. Conclusion: The present study describes H. longistipes morphologically and molecularly which facilitates further discrimination of this species worldwide.
Mostrar más [+] Menos [-]Taxonomic justification of the pathogenic strongylid infecting the Arabian camel Camelus dromedarius as Haemonchus longistipes by morphological and molecular phylogeny Texto completo
2019
Dajem Saad | Morsy Kareem | Al-Kahtani Mohammed | Abdel-Gaber Rewaida
Introduction: There is still lack of morphological and phylogenetic information on the pathogenic nematode of the camel Haemonchus longistipes. In the present study, this parasite was isolated in Saudi Arabia and described.
Mostrar más [+] Menos [-]Effect of body position and time on quantitative computed tomographic measurements of lung volume and attenuation in healthy anesthetized cats Texto completo
2018
Foo, Timothy S. | Pilton, Joanna L. | Hall, Evelyn J. | Martinez-Taboada, Ferando | Makara, Mariano
OBJECTIVE To quantify the effect of time and recumbency on CT measurements of lung volume and attenuation in healthy cats under general anesthesia. ANIMALS 8 healthy research cats. PROCEDURES Anesthetized cats were positioned in sternal recumbency for 20 minutes and then in left, right, and left lateral recumbency (40 minutes/position). Expiratory helical CT scan of the thorax was performed at 0 and 20 minutes in sternal recumbency and at 0, 5, 10, 20, 30, and 40 minutes in each lateral recumbent position. For each lung, CT measurements of lung volume and attenuation and the extent of lung areas that were hyperaerated (−1,000 to −901 Hounsfield units [HU]), normoaerated (−900 to −501 HU), poorly aerated (−500 to −101 HU), or nonaerated (−100 to +100 HU [indicative of atelectasis]) were determined with a semiautomatic threshold-based technique. A restricted maximum likelihood analysis was performed. RESULTS In lateral recumbency, the dependent lung had significantly greater attenuation and a lower volume than the nondependent lung. Within the dependent lung, there was a significantly higher percentage of poorly aerated lung tissue, compared with that in the nondependent lung. These changes were detected immediately after positioning the cats in lateral recumbency and remained static with no further significant time-related change. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that once anesthetized healthy cats were positioned in lateral recumbency, the dependent lung lobes underwent a rapid reduction in lung volume and increase in lung attenuation that did not progress over time, predominantly attributable to an increase in poorly aerated lung tissue.
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