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Changes of characterization of Salmonella Typhimurium isolate following sequential exposures to porcine neutrophil
2013
Lee, H.S., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kim, A., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Youn, M., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Lee, J., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Lim, S.K., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Kang, H.Y., Pusan National University, Pusan, Republic of Korea | Yoo, H.S., Seoul National University, Seoul, Republic of Korea | Park, J.W., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Wee, S.H., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Jung, S.C., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea
To develop a live vaccine candidate using an attenuated strain of Salmonella Typhimurium (ST), biochemical properties, plasmid profile, PFGE patterns and pathogenic analysis of the ST isolate were carried out after sequential passage of the ST isolate in porcine neutrophils. By the passage, the ability of the neutrophil-adapted isolate to utilize d-xylose was lost, while the ability of the strain to ferment trehalose was delayed after 2 or more days of the culture. Also, changes including deletion of the gene fragments were observed in PFGE analysis of the neutrophil-adapted isolates. Two plasmids, 105kb and 50kb, were cured in the strain passaged over 15 times in porcine neutrophils. The 50% of lethal dose (LD50) of the parent strain was changed from 1 × 105 LD50 to 6 × 106 LD50 by the passage in intraperitoneal injection of the strains into mice. These results suggested that bacterial genotypic and phenotypic responses might be globally altered depending on the inside environment of neutrophils.
Mostrar más [+] Menos [-]Evaluation of systemic and mucosal immune responses in mice administered with recombinant Salmonella Typhimurium expressing IutA protein
2013
Oh, I.G., Chonbuk National University, Jeonju, Republic of Korea | Choi, M., Chonbuk National University, Jeonju, Republic of Korea | Lee, J.H., Chonbuk National University, Jeonju, Republic of Korea
Avian pathogenic Escherichia coli (APEC) are known to cause extraintestinal disease in poultry, leading to substantial losses in the industry. IutA, iron-regulated aerobactin receptor is firmly associated with APEC. To assess the potential of IutA to induce protective immune responses, attenuated Salmonella Typhimurium strain expressing IutA was constructed and administered orally to BALB/c mice. The IutA-specific immune responses were measured with sera, vaginal and fecal samples by an enzyme-linked immunosorbent assay. We found that the Salmonella-IutA vaccine induced significantly higher immune responses as compared to the control inoculated with the attenuated S. Typhimurium containing the plasmid only. The IutA-specific immune responses were increased by second immunization at third week after initial immunization, whereas triple immunization induced lower immune responses than those induced by the double immunization. The Salmonella-IutA vaccine induced a nature of immunity biased to the Th1- type, as judged by the ratio of IutA-specific IgG isotypes (IgG2a/IgG1). Overall, these results suggest that the Salmonella-IutA vaccine appear to be suitable candidate for a vaccine against APEC. Keywords :
Mostrar más [+] Menos [-]Antibacterial activity of sodium phytate, sodium pyrophosphate, and sodium tripolyphosphate against Salmonella typhimurium in meats
2007
Hue, J.J. (Chungbuk National University, Cheongju, Republic of Korea) | Baek, D.J. (Chungbuk National University, Cheongju, Republic of Korea) | Lee, Y.E. (Chungbuk National University, Cheongju, Republic of Korea) | Lee, K.N. (Chungbuk National University, Cheongju, Republic of Korea) | Nam, S.Y. (Chungbuk National University, Cheongju, Republic of Korea) | Yun, Y.W. (Chungbuk National University, Cheongju, Republic of Korea) | Jeong, J.H. (Seowon University, Cheongju, Republic of Korea) | Lee, S.H. (Seowon University, Cheongju, Republic of Korea) | Yoo, H.S. (Seoul National University, Seoul, Republic of Korea) | Lee, B.J. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: beomjun@cbu.ac.kr
The approval of use of certain food-grade phosphates as food additives in a wide variety of meat products greatly stimulated research on the applications of phosphates in foods. Although phosphates have never been classified as antimicrobial agents, a number of investigators have reported that phosphates have antimicrobial activities. Phytic acid is a natural plant inositol hexaphosphate constituting 1-5% of most cereals, nuts, legumes, oil seeds, pollen, and spores. In this study, we investigated antibacterial activities of sodium phytate (SPT), sodium pyrophosphate (SPP), sodium tripolyphosphate (STPP) on Salmonella typhimurium in tryptic soy broth and in row meat media including chicken, pork and beef.
Mostrar más [+] Menos [-]Protective effects of mix-crude outer membrane protein Salmonella vaccine against salmonellosis in chickens and pigs
2007
Lee, H.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: leehsoo@nvrqs.go.kr | Lim, S.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Cho, Y.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Joo, Y.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, J.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, J.M. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
We investigated the safety, immunogenicity and protectivity of mix-crude outer membrane protein (cOMP) vaccine against salmonellosis in animals. The mix-cOMP vaccine was extracted from Salmonella enterica serovar Typhimurium (ST) and Salmonella enterica serovar Enteritidis (SE) and Salmonella enterica serovar Braenderup (SB) isolated from pigs. The mix-cOMP vaccine gave significantly higher antibody response than ST-bacterin and ST-cOMP vaccine in guinea pigs. The survival rates of mix-cOMP vaccinated groups showed significantly higher (100%) than those (0-20%) of unvaccinated control group, challenged with 3 species of Salmonella (ST, SE and SB) in mice.
Mostrar más [+] Menos [-]Prevalence and pathologic study of porcine salmonellosis in Jeju
2017
Yang, H.S., Jeju Self-Governing Provincial Veterinary Research Institute, Jeju, Republic of Korea | Kang, S.C., Optipharm Inc., Cheongju, Republic of Korea | Kim, A.R., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Jung, B.Y., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Kim, J.H., Jeju National University, Jeju, Republic of Korea
Salmonella (S.) Typhimurium is highly contagious, and its infection may rapidly spread within pig populations of herd. According to the survey (1,191 pigs) from 2003 to 2012, 155 pigs (13.0%) were diagnosed as salmonellosis in Jeju. Major porcine salmonellosis cases (88.4%) were concentrated in 4- to 12-week-old weaned pigs, but 6 pigs (3.9%) under 4 weeks old were also diagnosed. Based on the histopathologic examinations, ulcerative enteritis (63.9%) in the large intestine and/or paratyphoid nodules formation (57.4%) in the liver were most prevalent lesions in porcine salmonellosis. Single infection of S. Typhimurium and mixed infection with more than 2 pathogens were detected in 38 (24.5%) and 117 (75.5%) in pigs, respectively. Co-infections of Porcine reproductive and respiratory syndrome virus and Porcine circovirus type 2 were very common in porcine salmonellosis in Jeju and detected in 84 (54.2%) and 59 (38.1%) pigs, respectively. Based on the serotyping tests using 41 bacterial isolates, S. Typhimurium and S. Rissen were confirmed in 39 (95.1%) and 2 (4.9%) cases, respectively.
Mostrar más [+] Menos [-]Studies on Salmonella isolated from chicks
1994
Oh, G.H. (Kyungpook Veterinary Service Laboratory, Taegu (Korea Republic)) | Choi, W.P. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine)
Effect of Bupleurum falcatum extract on cellular immune responses
1993
Chung, Y.M. | Kim, J.M. | Song, H.J. | Cho, J.K. (Chonpook National University, Chonju (Korea Republic). College of Veterinary Medicine)