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Characterisation and antibiotic resistance of Yersinia enterocolitica from various meat categories, South Africa
2022
Seakamela,Emmanuel M. | Diseko,Letlhogonolo | Malatji,Dikeledi | Makhado,Lavhelesani | Motau,Mmatau | Jambwa,Kudakwashe | Magwedere,Kudakwashe | Ntushelo,Nombasa | Matle,Itumeleng
Yersinia enterocolitica infections impose a significant public health and socioeconomic burden on human population in many countries. The current study investigated the prevalence, antimicrobial resistance profile and molecular diversity of Y. enterocolitica in meat and meat products across various retail outlets in selected provinces of South Africa (SA). In a cross-sectional study, a total of 581 retail meat and meat products were collected from four cities across three provinces of SA. Samples were from beef and pork products, which included 292 raw intact, 167 raw processed, and 122 ready-to-eat (RTE) meats. Samples were analysed using classical microbiological methods for isolation, identification and biotyping of Y. enterocolitica. Conventional polymerase chain reaction (PCR) was performed for confirmation, serotyping, screening of virulence (n = 11) and antimicrobial resistance (n = 18) genes. Phenotypic antimicrobial resistance profiles were determined against 12 antibiotics discs, using disc diffusion method. The overall prevalence of 12% (70/581) was reported across all cities with contamination proportion reported in samples collected from raw intact 15% (43/292), followed by raw processed 11% (18/167) and RTE meats 7% (9/122). All positive isolates were of biotype 1A with 7% (5/70) belonging to bioserotype 1A/O:8. Most of the isolates harboured ymoA, ystB, fepD, ail, fepA, invA and myfA virulence genes. High antimicrobial resistance frequency was observed for ampicillin (94%), cephalothin (83%) and amoxicillin (41%), respectively. Of the 18 tested antimicrobial resistance genes, blaTEM was the most predominant (40%) followed by cmlA (21%). This study reveals the presence of antimicrobial resistant Y. enterocolitica possessing virulent genes of public health importance in products of animal origin, therefore, health monitoring and surveillance of this pathogen is required.
Mostrar más [+] Menos [-]Prevalence of serotype specific antibody to equine encephalosis virus in Thoroughbred yearlings in South Africa (1999-2004)
2008
Howell, P.G.(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Nurton, Jane P.(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Nel, Daleen(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Lourens, Carina W.(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Guthrie, A.J.(University of Pretoria Faculty of Veterinary Science Equine Research Centre)
Cohorts of yearlings were sampled over a period of 6 years in a retrospective serological survey to establish the annual prevalence of serotype specific antibody to equine encephalosis virus on Thoroughbred stud farms distributed within defined geographical regions of South Africa. Seasonal seroprevalence varied between 3.6 % and 34.7 %, revealing both single and multiple serotype infections in an individual yearling. During the course of this study serotypes 1 and 6 were most frequently and extensively identified while the remaining serotypes 2, 3, 4, 5 and 7 were all identified as sporadic and localized infections affecting only individual horses. This study of the seasonal prevalence of equine encephalosis virus has a corollary and serves as a useful model in the seasonal incidence of the serotypes of African horse sickness and bluetongue in regions where the respective diseases are endemic.
Mostrar más [+] Menos [-]The testing and modification of a commercially available transport medium for the transportation of pure cultures of Haemophilus paragallinarum for serotyping
2004
Bragg, R.R. | Jansen van Rensburg, P. | Van Heerden, E. | Albertyn, J. (Free State Univ., Bloemfontein (South Africa). Microbial, Biochemical and Food Biotechnology Dept.)
Virulence of South African isolates of Haemophilus paragallinarum. Part 1: NAD-dependent field isolates
2002
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbiology and Biochemistry Dept.)
Isolation of serovar C-3 Haemophilus paragallinarum from Zimbabwe: A further indication of the need for the production of vaccines against infectious coryza containing local isolates of H. paragallinarum
2002
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbiology and Biochemistry Dept.)
The distribution of Pasteurella haemolytica serotypes among cattle, sheep and goats in South Africa and their association with disease
1995
Odendaal, M.W. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Henton, M.M.
Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001
2004
Sahle, M. | Venter, E.H. (Pretoria Univ., Onderstepoort (South Africa). Veterinary Tropical Diseases Dept.) | Dwarka, R.M. | Vosloo, W.
The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa
2002
Howell, P.G. (Pretoria Univ., Onderstepoort (South Africa). Equine Research Centre) | Groenewald, D. | Visage, C.W. | Bosman, A.-M. | Coetzer, J.A.W. | Guthrie, A.J.
Virulence of South African isolates of Haemophilus paragallinarum. Part 2: Naturally occuring NAD-independent field isolates
2002
Bragg, R.R. (Free State Univ., Bloemfontein (South Africa). Microbiology and Biochemistry Dept.)
A monovalent attenuated serotype 2 bluetongue virus vaccine confers homologous protection in sheep
2001
Hunter, P. | Modumo, J. (Onderstepoort Biological Products (South Africa))