Objective: This study was conducted to determine the prevalence of Salmonella in milk (farm bulk milk, raw market milk) and cheese (kareish, white soft cheese) samples that were collected randomly from farms, supermarkets, small vendors and shops in different districts of Mansoura city, Egypt.Materials and methods: A total of 100 farm bulk milk, raw market milk, kareish cheese and white soft cheese samples (25 of each) were screened for the prevalence of Salmonella spp. The Salmonella isolates were isolated and identified by conventional bacteriological techniques, which were further confirmed genetically by polymerase chain reaction (PCR) based on the presence of invA gene. Finally, the isolates were serotyped.Results: Salmonella could be detected in 15%(n=15/100) samples with a prevalence of 12%(n=3/25), 24%(n=6/25), 20%(n=5/25) and 4%(n=1/25) in raw market milk, raw farm bulk milk, kareish cheese and white soft cheese, respectively. The Salmonella isolates were serotyped into S. enteritidis 33.3%(n=9/27) which was the most frequent, followed by S. typhimurium 25.9%(n=7/27), S. heidelberg 14.8%(n=4/27), S. infantis 11.11%(n=3/27), S. tsevie 11.11%(n=3/27) and S. haifa 3.7%(n=1/27).Conclusion: The present study confirms the presence of Salmonella in milk and cheese samples in Mansoura, Egypt, indicating that the dairy products can act as potential sources of Salmonella infection. Thus, appropriate hygienic measures are suggestive for combating Salmonellosis in Egypt. [J Adv Vet Anim Res 2017; 4(1.000): 45-51]

Escherichia coli infections cause significant financial losses for the worldwide chicken economy, so this research is being conducted to evaluate the efficacy of the inactivated multivalent Escherichia coli vaccine. Six groups were created from a total of 120 broiler chicks; the groups (1 and 3) were received E. coli inactivated vaccine at day 7 and challenged with O78 and O128 respectively, groups 2 and 4 were referred to as the control of challenge for the Escherichia coli strains O78 and O128. Groups 5 and 6 were maintained as the non-vaccinated and non-challenged control negative group and vaccinated non challenged group respectively. Challenged with E. coli strains of heterologous O128 and homologous O78 were given at 28 days old broilers by injection into the muscle of the thigh with 0.2ml contained 107 CFU / bird. Moreover, the performance measurements of body weight per gram, feed intake and feed conversion ratio were measured; histopathological findings, clinical symptoms, rate of mortality and total intestinal counts of Escherichia coli were also examined. The study revealed that the vaccination of COLI-VAC™ E. coli provided a significant defense against the challenge between homologous O78 strain and heterologous O128 strain. Just used the aforementioned parameters, Groups 1 and 3 that vaccinated at seven days old and challenged at 28 days old chicks with homologous and heterologous (O78 and O128) serotypes of E. coli provided impressive protection against all those non-vaccinated chicks (Groups 2 and 4) that were challenged with O78 and O128 serotypes of E. coli.