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Studies on the diagnosis of subclinical mastitis in cows by the measurement of the electrical conductivity, 1; comparison of various methods of handling conductivity data with the use of California mastitis test and direct somatic cell count
1984
Kang, B.K. (Chonnam National Univ., Kwangju (Korea R.). Coll. of Agriculture. Dept. of Veterinary Medicine)
A total of 466 foremilk from dairy farms in Chonnam Korea [R.] district was examined for the subclinical mastitis over a period of 1 year, using a method of the electrical conductivities (EC); absolute conductivity (AC) and differential conductivity (DC) and quarter difference value (QD), in relation to the California mastitis test (CMT) and the direst somatic cell count (DSCC). The compatibility and efficiency rating between the EC values and the other screening tests was conducted
Mostrar más [+] Menos [-]Studies on the factors influencing the transformation in Escherichia coli with pBR 322 DNA
1984
Yoo, H.S. | Mah, J.S. (Seoul National Univ., Suwon (Korea R.). Coll of Veterinary Medicine)
To investigate the factors influencing the artificial transformation in Escherichia coli, E. coli C 600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCL-EtBr equilibrium density gradient centrifugation from E. coli HB 101. The best conditions for the transformation of E. coli C 600 with pBR322 DNA were treatment with 100mM CaCl2, ice incubation at 0 deg C for 45 minutes, heat shock at 42 deg C for 4 min., 30 min. of ice incubation and incubation at 37 deg C for 100 min. for gene expression in that order
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