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Sequential pathologic changes and viral distribution in rabbits experimentally infected with new Korean strain of rabbit hemorrhagic disease virus (RHDVa)
2012
Park, J.W., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Chun, J.E., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Yang, D.K., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Bak, E.J., Yonsei University, Seoul, Republic of Korea | Kim, H., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Lee, M.H., Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Hwang, E.K., Sangji University, Wonju, Republic of Korea | Lee, C.B., Konkuk University, Seoul, Republic of Korea | Woo, G.H., Semyung University, Jecheon, Republic of Korea
Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.
Mostrar más [+] Menos [-]Histopathological observations and virus detection by in situ hybridizatio in wild rats intranasally infected with Aujeszky's disease virus isolated in Korea
1999
Song, G.S. (Yuhan Research Center, Kunpo (Korea Republic).) | Moon, O.K. (Ministry of Agriculture Forestry, Anyang (Korea Republic). National Veterinary Research and Quarantine Service) | Jeong, C.G. | Kim, S.B. (Gyeongsang National University, Chinju (Korea Republic). Institute of Animal Medicien, College of Veterinary Medicine)
The present study was carried out to investigate the pathogenicity and pathogenesis of wild rats(Rattus norvegicus), trapped in nature, intranasally infected Aujeszky's disease virus(ADV/NYJ-1-87) by histopathology, immunohistochemistry and in situ hybridization(ISH). Fifteen rats inoculated intranasally were roughened haircoat, anorexia, listlessness, and depression second day after inoculation, and three rats died in 66-72 hours. Eight rats showed severe pruritus at the fact that was accompanied by frequent face-washing movements of the forelegs, and then became violent and spasmodic for and hour or until they died. Four rats slowly recovered after showing mild clinical signs of the disease. Microscopic lesions in infected rats were characterized by meningitis, perivascular round cell infiltration, focal gliosis, and neuronal degeneration and necrosis. And intranuclear inclusion bodies were frequently detected in the cerebral cortex and medulla. Positive reaction to ADV by immunohistochemistry and ISH were detected in the following areas:trigemimal ganglion, brain, tonsil, nasal mucosa, spleen, lung and liver. The result has suggested that ADV intranasally infected in wild rats is followed by replication in epithelial cells of nasal mucosa and tonsil, then invade local lymph nodes by way of the lymphatics. It is also believed that the virus invades bipolar olfactory cells and trigerminal ganglion, and then spread into central nervous system.
Mostrar más [+] Menos [-]Prevalence for persistent infection with bovine viral diarrhea virus in Korean native calves
2007
Bae, Y.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: baeyc@nvrqs.go.kr | Kim, H.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Park, J.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Woo, G.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, O.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kang, M.I. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Bovine viral diarrhea (BVD) is very important disease in cattle industry with a worldwide distribution. Detection and elimination of persistently infected calves with bovine viral diarrhea virus (BVDV) was valuable strategy for BVD eradication because those calves were main source for transmission. We surveyed persistent infection with BVDV by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) using whole blood and skin. Five hundred thirty nine Korean native calves were tested. Four calves (0.7%) were positive for BVDV antigen for both tests.
Mostrar más [+] Menos [-]Sequence analysis of the variable VP2 gene of infectious bursal disease viruses isolated in Korea
1999
Kwon, H.M. | Kim, D.K. (Kangwon National University, Chuncheon (Korea Republic). Department of Veterinary Medicine) | Seong, H.W. (National Veterinary Research and Quarantine Service, Anyang (Korea Republic).)
A 474-base pair segment covering the hypervaible region of VP2 gene from six Korean infectious bursal disease virus(K-IBDV) isolates(K1, K2, SH/92, 225, 269, 310) and one attenuated IBDV(DAE) were amplified using RT-PCR, sequenced, and compared with published sequences for IBDV. K-IBDV isolates(K1, K2, SH/92, 225, 269) and foreigh very virulent(vv) IBDV strains had 94.93-100% amino cid sequence similarity. K-IBDV isolate 310 and other K-IBDV isolates had 84.31%-86.07% amino acid sequence similarity. Attenuated strain(DAE), like other attenuated strain, has substitution at positions 279(D to N) and 284(A to T) as well as in the serine-rich heptapeptide region. Five K-IBDV isolates except 310 isolate share unique amino acid residues at positions 222(A), 256(I), 294(I) which are not present in other standard and attenuated strains. At the two hydrophilic region, K-IBDV isolates except 310 isolate had identical amino cids comparing with Belgium vv IVDV 894VB but had four amino acid substitutions comparing with Chinese vv IBDV F9502. The SWSASGS heptapeptide is conserved in all KIBDV isolates. The sequence of K-IBDV isolate 310 was markedly different from other IBDV strains, evolving from a separate lineage than the others. By phylogenetic analysis, Five K-IBDV isolates except 310 isolate were categorized in one group with foreign vv IBDV isolates but K-IBDV isolate 310 was categorized ina separate group which was differentiated form other compared IBDV strains
Mostrar más [+] Menos [-]Characterization of infectious bursal disease viruses isolated in Korea using RT/PCR and RFLP analysis
1999
Kwon, H.M. | Kim, D.K. (Kangwon National University, Chuncheon (Korea Republic). Department of Veterinary Medicine) | Seong, H.W. (National Veterinary Research and Quarantine Service, Anyang (Korea Republic).)
Field infectious bursal disease viruses 9IBDVs) were isolated from IBDV-suspected commercial chickens. The variable region in VP2 gene of six Korean IBDV isolates (K-IBDVs) and IBD vaccines was examined using the reverse transcriptase/polymerase chain reaction-restriction fragment length polymorphism (RT/PCR-RFLP) assay. With all K-IBDVs and vaccine IBDVs, a 474-bp fragment of the VP2 gene was amplified and tested with various restriction enzymes. Resseriction enzymes BstNI and StyI differentiated K-IBDV isolates and IBD vaccines into four groups. Reseriction enzyme profiles of K-IBDV isolates were different from them of IBD vaccines. K-IBDV isolates except for 310 isolate had specific SspI and TaqI recognition sites, which were recognized in highly virulent IBDVs, but IBD vaccines had no those sites. This study showed that RT/PCR-RFLP assay was thought to be valuable tool for differentiation of IBDVs and identification of highly virulent IBDV.
Mostrar más [+] Menos [-]Detection of bovine viral diarrhea virus by In situ hybridization
1999
Park, N.Y. | Hong, K.K. | Chung, C.Y. | Cho, K.O. | Lee, B.J. | Park, Y.S. | Park, H.S. (Chonnam National Univerisyt, Kwangju (Korea Republic). College of Veterinary Medicine) | Kweon, C.H. (National Veterinary Research and Quarantine Service, Anyang (Korea Republic).)
Detection and distribution of bovine biral diarrhea virus(BVDV) was studied in formalin-fixed, paraffin-embedded tissues from two naturally infected cattle by in situ hybridization with a non-radioactive biotinylated probe. A 600 base pair cDNA probe from BVDV B-25 strain ws used for probe. The whole procedure of ISH to diagnose was carried out within 1~2 hours in MicroprobeTM capillary action system. The biotin-labelled probe was demonstrated after hybridization under standard conditions by the application of streptoavidin and biotinylated alkaline phosphatase. Alkaline phosphatase was visualized using a fast red TR/naphthol phosphatase and the sections were counterstained with hematoxylin. We have obtained the result of positive reactions in digestive tract(sm1.all intestine and colon) and epidermis of tongue in the state of the intract tissues. The result suggested that in situ hybridization method can be considered as a useful diagnostic technique for detection of specific nucleic acid sequences of BVDV.
Mostrar más [+] Menos [-]Isolation of porcine reproductive and respiratory syndrome virus (PRRSV) in Korea
1994
Kweon, C.H. | Kwon, B.J. | Lee, H.J. | Cho, J.J. | Hwang, E.K. | Shin, J.H. | Yoon, Y.D. | Kang, Y.B. | An, S.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute) | Kim, Y.H. (Bayer Veterinary Medical Research Institute, Anyang (Korea Republic)) | Huh, W. (Daesung Laboratory, Anyang (Korea Republic)) | Jun, M.H. (Chungnam National University, Taejon (Korea Republic). College of Veterinary Medicine) | Wensvoort, G. (Central Veterinary Institute, Lelystad (Netherlands))
Detection of antibodies against infectious Borna disease virus - A comparison of three serological methods-
1992
Lee, D.S. (Cheju Nat'l Univ., Cheju (Korea Republic). Dept. of Veterinary Medicine)
Biophysical characteristics of a noncytopathic bovine viral diarrhea virus
1992
Kweon, C.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Inst.) | Anthony, C.E. (Univ. of California Davis, California (USA). California Veterinary Lab.) | Woo, H.J. (Harvard Medical School, Boston (USA). Lab. of Cancer Biology)
Pig viral diseases causing reproductive failure in Korea
1992
Kim, B.H. | Kweon, C.H. | An, S.H. | Rhee, J.C. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)