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Systemic Aspergillosis associated with bovine viral diarrhea virus infection in Korean native calves
2005
Jean, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: jean@nvrqs.go.kr | Kim, J.H. (Cheju National University, Jeju, Republic of Korea) | Kim, D.Y. (Seoul National University, Seoul, Republic of Korea) | Jeong, S.W. (Konkuk University, Seoul, Republic of Korea) | Cho, D.Y. (Louisiana State University, Baton Rouge, LA, USA) | Moon, O.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Systemic aspergillosis were outbroken of 17 Korean native calves at livestock farm in Kyunggi province. Two 3 months old calves were died after having diarrhea, coughing, dyspnea, nasal discharge, and abdominal breathing. These calves were diagnosed of aspergillosis as multifocal to coalescing pyogranulomatous inflammation with intralesional aspergillus infiltration at liver, kidney, spleen, heart, lymph nodes, lungs, intestine and cerebrum. Aspergillus fumigatus was cultured from the affected tissues. And the small intestine was positive against bovine viral diarrhea (BVD) virus on FA test. We considered that the secondary fungal infections killed the calves after BVD virus infection primarily.
Mostrar más [+] Menos [-]Detection of bovine viral diarrhea virus by In situ hybridization
1999
Park, N.Y. | Hong, K.K. | Chung, C.Y. | Cho, K.O. | Lee, B.J. | Park, Y.S. | Park, H.S. (Chonnam National Univerisyt, Kwangju (Korea Republic). College of Veterinary Medicine) | Kweon, C.H. (National Veterinary Research and Quarantine Service, Anyang (Korea Republic).)
Detection and distribution of bovine biral diarrhea virus(BVDV) was studied in formalin-fixed, paraffin-embedded tissues from two naturally infected cattle by in situ hybridization with a non-radioactive biotinylated probe. A 600 base pair cDNA probe from BVDV B-25 strain ws used for probe. The whole procedure of ISH to diagnose was carried out within 1~2 hours in MicroprobeTM capillary action system. The biotin-labelled probe was demonstrated after hybridization under standard conditions by the application of streptoavidin and biotinylated alkaline phosphatase. Alkaline phosphatase was visualized using a fast red TR/naphthol phosphatase and the sections were counterstained with hematoxylin. We have obtained the result of positive reactions in digestive tract(sm1.all intestine and colon) and epidermis of tongue in the state of the intract tissues. The result suggested that in situ hybridization method can be considered as a useful diagnostic technique for detection of specific nucleic acid sequences of BVDV.
Mostrar más [+] Menos [-]Differential diagnosis among Marek's disease, reticuloendotheliosis and avian leukosis by polymerase chain reaction
1998
Seong, H.W. (National Veterinary Research Institute, RDA, Anyang (Korea Republic)) | Kim, S.J. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
An epidemiological survey on seroprevalence of vector mediated virus infection in cattle bred in a Japanese remote island, Okinawa
1998
Takeo, S. | Masaaki H. | Sakae, A. | Keiichi, F. (Nihon University, Fujisawa (Japan). Department of Preventive Veterinary Medicine) | Fumio, H.I. (Saopaulo University, Saopaulo (Brazil). Department of Veterinary Preventive Medicine) | Lee, W.C. | Lee, J.B. (Konkuk University, Seoul (Korea Republic). Department of Veterinary Public Health)
Studies on the cloning gp50 and gp63 genes of Pseudorabies virus (Shope strain)
1991
Kweon, C.H. | An, S.H. | Song, J.Y. | Kim, B.H. | Lee, J.B. | Lee, J.C. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute) | Maeda, S. (Univ. of California, Davis (USA))
RT-PCR and nested PCR amplification of the PRRSV genes from boar semen for the rapid and sensitive differential diagnosis
1998
Lyoo, Y.S. (Konkuk University, Seoul (Korea Republic). Veterinary Medicine) | Park, C.K. | Lee, C.H. (National Veterinary Research Institute, Anyang (Korea Republic))
