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Serum total antioxidant capacity and enzymatic defence of dogs with chronic heart failure and atrial fibrillation: a preliminary study Texto completo
2020
Michałek Marcin | Tabiś Aleksandra | Noszczyk-Nowak Agnieszka
Serum total antioxidant capacity and enzymatic defence of dogs with chronic heart failure and atrial fibrillation: a preliminary study Texto completo
2020
Michałek Marcin | Tabiś Aleksandra | Noszczyk-Nowak Agnieszka
Atrial fibrillation may potentially contribute to oxidative stress to a greater extent than chronic heart failure. The aim of the study was to compare the serum total antioxidant capacity and enzymatic antioxidant defence of dogs with chronic heart failure and atrial fibrillation with those of subjects with chronic heart failure and sinus rhythm and healthy controls.
Mostrar más [+] Menos [-]Serum total antioxidant capacity and enzymatic defence of dogs with chronic heart failure and atrial fibrillation: a preliminary study Texto completo
2020
Michałek, Marcin | Tabiś, Aleksandra | Noszczyk-Nowak, Agnieszka
Atrial fibrillation may potentially contribute to oxidative stress to a greater extent than chronic heart failure. The aim of the study was to compare the serum total antioxidant capacity and enzymatic antioxidant defence of dogs with chronic heart failure and atrial fibrillation with those of subjects with chronic heart failure and sinus rhythm and healthy controls. A total of 33 dogs were divided into three groups: dogs with chronic heart failure and atrial fibrillation (CHF + AF; n = 12), chronic heart failure and sinus rhythm (CHF + SR; n = 9), and healthy controls (n = 12). Serum total antioxidant capacity (TAC), serum CuZn-superoxide dismutase (SOD) and catalase, and plasma glutathione peroxidase (GPx) activity were determined. SOD activity and serum TAC were significantly lower in the study groups than in control animals. Catalase activity was significantly higher and plasma GPx activity significantly lower in dogs with CHF + AF compared with the CHF + SR and control dogs. The results suggest that chronic heart failure in dogs significantly impacts the serum TAC and the antioxidant enzymatic defence, while plasma GPx activity is markedly lower in dogs with chronic heart failure and atrial fibrillation. The role of that imbalance needs further investigation.
Mostrar más [+] Menos [-]Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing Texto completo
2020
Escárcega Dulce Anahy Verdugo | Razo Claudia Angélica Perea | Ruíz Sara González | Gallegos Susana Lucia Sosa | Suazo Feliciano Milián | Alarcón Germinal Jorge Cantó
Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing Texto completo
2020
Escárcega Dulce Anahy Verdugo | Razo Claudia Angélica Perea | Ruíz Sara González | Gallegos Susana Lucia Sosa | Suazo Feliciano Milián | Alarcón Germinal Jorge Cantó
Bovine tuberculosis, caused by M. bovis, is endemic in Mexico and has had a big impact on public health. Jalisco is considered to be an important dairy region in the country, accounting for approximately 19% of the total milk production. Within Jalisco, the region of Altos Sur holds the largest proportion of the cattle inventory of the state.
Mostrar más [+] Menos [-]Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing Texto completo
2020
Escárcega, Dulce Anahy Verdugo | Razo, Claudia Angélica Perea | Ruíz, Sara González | Gallegos, Susana Lucia Sosa | Suazo, Feliciano Milián | Alarcón, Germinal Jorge Cantó
Bovine tuberculosis, caused by M. bovis, is endemic in Mexico and has had a big impact on public health. Jalisco is considered to be an important dairy region in the country, accounting for approximately 19% of the total milk production. Within Jalisco, the region of Altos Sur holds the largest proportion of the cattle inventory of the state. To determine the frequency of bovine tuberculosis in Altos Sur, Jalisco, as well as M. bovis genetic diversity, sampling of tissue (lymph nodes, lungs, and liver) from Holstein cattle was performed in four abattoirs belonging to three municipalities of this region (Tepatitlán de Morelos, San Miguel el Alto, and Arandas). Spoligotyping and whole-genome sequencing were carried out to assess the genetic relationships of M. bovis strains circulating in this area, as well as a comparison to isolates from other places in Mexico. Prevalence was 15.06%, and distribution similar among the three municipalities. The most frequent spoligotypes were SB0673, SB121, and SB0145. Whole-genome sequencing revealed three main clades (I, II, III), but isolates did not show clustering by region. Phylogenetic analysis suggested ongoing transmission between herds of the different regions, and no unique source of infection was determined. This hinders efforts under the national program for the control and eradication of the disease, so serious attention must be paid to rural regions such as Altos Sur in order to improve its success.
Mostrar más [+] Menos [-]Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway Texto completo
2020
Luo Yi-Ran | Zhou Shu-Ting | Yang Liang | Liu Yuan-Ping | Jiang Sheng-Yao | Dawuli Yeliboli | Hou Yi-Xuan | Zhou Tian-Xing | Yang Zhi-Biao
Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway Texto completo
2020
Luo Yi-Ran | Zhou Shu-Ting | Yang Liang | Liu Yuan-Ping | Jiang Sheng-Yao | Dawuli Yeliboli | Hou Yi-Xuan | Zhou Tian-Xing | Yang Zhi-Biao
Porcine epidemic diarrhoea virus (PEDV) infection causes watery diarrhoea, vomiting, anorexia, and weight loss, especially among neonatal piglets, inflicting on them morbidity and mortality potentially reaching 90%–100%. Despite it being known that certain mammalian cell phases are arrested by PEDV, the mechanisms have not been elucidated, and PEDV pathogenesis is poorly understood. This study determined the effect of an epidemic PEDV strain on cell cycle progression.
Mostrar más [+] Menos [-]Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway Texto completo
2020
Luo, Yi-Ran | Zhou, Shu-Ting | Yang, Liang | Liu, Yuan-Ping | Jiang, Sheng-Yao | Dawuli, Yeliboli | Hou, Yi-Xuan | Zhou, Tian-Xing | Yang, Zhi-Biao
Porcine epidemic diarrhoea virus (PEDV) infection causes watery diarrhoea, vomiting, anorexia, and weight loss, especially among neonatal piglets, inflicting on them morbidity and mortality potentially reaching 90%–100%. Despite it being known that certain mammalian cell phases are arrested by PEDV, the mechanisms have not been elucidated, and PEDV pathogenesis is poorly understood. This study determined the effect of an epidemic PEDV strain on cell cycle progression. We observed the effect of the PEDV SHpd/2012 strain on an infected Vero cell cycle through flow cytometry and Western blot, investigating the interrelationships of cell-cycle arrest, the DNA damage–signalling pathway caused by PEDV and the phosphorylation levels of the key molecules Chk.2 and H2A.X involved upstream and downstream in this pathway. PEDV induced Vero cell-cycle arrest at the G1/G0 phase. The phosphorylation levels of Chk.2 and H2A.X increased with the prolongation of PEDV infection, and no significant cell-cycle arrest was observed after treatment with ATM or Chk.2 inhibitors. The proliferation of PEDV was also inhibited by treatment with ATM or Chk.2 inhibitors. PEDV-induced cell-cycle arrest is associated with activation of DNA damage–signalling pathways. Our findings elucidate the molecular basis of PEDV replication and provide evidence to support further evaluation of PEDV pathogenesis.
Mostrar más [+] Menos [-]Effects of α-enolase gene silencing on reproductive-related hormone receptor expression and steroid hormone synthesis of primary granulosa cells from goose F1 follicles Texto completo
2020
Ji Hong | Niu Chun-Yang | Zhang Hong-Liang | Guo Jing-Ru | Zhen Li | Lian Shuai | Yang Chuang | Yang Huan-Min | Wang Jian-Fa
Effects of α-enolase gene silencing on reproductive-related hormone receptor expression and steroid hormone synthesis of primary granulosa cells from goose F1 follicles Texto completo
2020
Ji Hong | Niu Chun-Yang | Zhang Hong-Liang | Guo Jing-Ru | Zhen Li | Lian Shuai | Yang Chuang | Yang Huan-Min | Wang Jian-Fa
Enolases are enzymes in the glycolytic pathway, which catalyse the reversible conversion of D-2-phosphoglycerate into phosphoenol pyruvate in the second half of the pathway. In this research, the effects of α-enolase (ENO1) on steroid reproductive-related hormone receptor expression and on hormone synthesis of primary granulosa cells from goose F1 follicles were studied.
Mostrar más [+] Menos [-]Effects of α-enolase gene silencing on reproductive-related hormone receptor expression and steroid hormone synthesis of primary granulosa cells from goose F1 follicles Texto completo
2020
Ji, Hong | Niu, Chun-Yang | Zhang, Hong-Liang | Guo, Jing-Ru | Zhen, Li | Lian, Shuai | Yang, Chuang | Yang, Huan-Min | Wang, Jian-Fa
Enolases are enzymes in the glycolytic pathway, which catalyse the reversible conversion of D-2-phosphoglycerate into phosphoenol pyruvate in the second half of the pathway. In this research, the effects of α-enolase (ENO1) on steroid reproductive-related hormone receptor expression and on hormone synthesis of primary granulosa cells from goose F1 follicles were studied. Primary granulosa cells from the F1 follicles of eight healthy 8-month-old Zi geese were separated and cultured. An ENO1 interference expression vector was designed, constructed and transfected into primary cultured granulosa cells. The mRNA expression levels of follicle-stimulating hormone receptor (FSHR), luteinising hormone receptor (LHR), oestrogen receptor α (ER α), oestrogen receptor β (ER β), growth hormone receptor (GHR) and insulin-like growth factor binding protein-1 (IGFBP-1) in the cells were evaluated as were the secretion levels of oestradiol, activin, progesterone, testosterone, inhibin and follistatin in cell supernatant. α-enolase gene silencing reduced the expression of FSHR, LHR, ERα, ERβ, GHR, and IGFBP-1 mRNA, potentiated the secretion of oestrogen, progesterone, testosterone, and follistatin of granulosa cells, and hampered the production of activin and inhibin. ENO1 can regulate the reactivity of granulosa cells to reproductive hormones and regulate cell growth and development by adjusting their hormone secretion and reproductive hormone receptor expression. The study provided a better understanding of the functional action of ENO1 in the processes of goose ovary development and egg laying.
Mostrar más [+] Menos [-]Utility of urinary markers in the assessment of renal dysfunction in familial glomerulonephritis in Dobermann dogs Texto completo
2020
Winiarczyk Dagmara | Adaszek Łukasz | Madany Jacek | Winiarczyk Mateusz | Winiarczyk Stanisław
Dobermann dogs are reportedly predisposed to familial glomerulonephropathy. Proteinuria is a hallmark of canine familial glomerular diseases. The identification of glomerular abnormalities in breeds so predisposed is of great importance in improving breeding policy. Therefore, markers that allow the detection and localisation of renal damage are needed. The purpose of this study was to investigate the urinary concentrations of immunoglobulin G (uIgG), retinol-binding protein (uRBP), and Tamm–Horsfall protein (uTHP) in a family of Dobermanns with proteinuria and compare these concentrations with the corresponding values in healthy controls.
Mostrar más [+] Menos [-]Therapeutic effects of revascularisation on the healing of free bone grafts in dogs Texto completo
2020
Zheng Jia-San | Ruan Hong-Ri | Shuang-Qiu | Jing-Nie | Hou Kai-Wen | Rui-Wu
Therapeutic effects of revascularisation on the healing of free bone grafts in dogs Texto completo
2020
Zheng Jia-San | Ruan Hong-Ri | Shuang-Qiu | Jing-Nie | Hou Kai-Wen | Rui-Wu
The therapeutic effect of subcutaneous embedding and revascularisation on the repair of canine bone defects caused by open fracture was examined.
Mostrar más [+] Menos [-]Therapeutic effects of revascularisation on the healing of free bone grafts in dogs Texto completo
2020
Zheng, Jia-San | Ruan, Hong-Ri | Shuang-Qiu, | Jing-Nie, | Hou, Kai-Wen | Rui-Wu,
The therapeutic effect of subcutaneous embedding and revascularisation on the repair of canine bone defects caused by open fracture was examined. A total of 12 adult beagle dogs were randomly split into a control group (group C) and a test group (group T). A section of the radius was removed from each dog under general anaesthesia and the deficit supported by an orthopaedic implant. Group T had the section surgically implanted next to the blood vessel–rich saphenous vein and Group C had it cryopreserved at −80°C. After eight weeks, the bone was surgically implanted back into the matching radial deficit. Bone healing was evaluated by gross morphological and X-ray examinations, post-mortem histology, and successive blood measurements of key bone biochemical markers. At 12 weeks, the bone healing boundary was disappearing more quickly in group T dogs than in their group C counterparts. X-ray and histological examinations showed that the cortical repair of group T subjects was complete and the bony plate arrangement was more regular than that in group C. The levels of bone biochemical markers also proved that the healing state of group T was better. The results showed that the degree of healing, osteoclast activity, and bone formation status of group T were better than those of group C, proving that the vascularised bone graft had a significantly shorter healing time than the cryopreserved bone graft.
Mostrar más [+] Menos [-]Fatty acid profile in edible eggs of snails from the Cornu genus Texto completo
2020
Maćkowiak-Dryka Monika | Szkucik Krzysztof | Ziomek Monika | Klimek Kamila
Fatty acid profile in edible eggs of snails from the Cornu genus Texto completo
2020
Maćkowiak-Dryka Monika | Szkucik Krzysztof | Ziomek Monika | Klimek Kamila
The aim of this study was to determine the content of fatty acids in eggs harvested from two edible subspecies of Polish-bred common garden snail from the Cornu genus, as well as this content in the retail-ready product obtained from these eggs.
Mostrar más [+] Menos [-]Fatty acid profile in edible eggs of snails from the Cornu genus Texto completo
2020
Maćkowiak-Dryka, Monika | Szkucik, Krzysztof | Ziomek, Monika | Klimek, Kamila
The aim of this study was to determine the content of fatty acids in eggs harvested from two edible subspecies of Polish-bred common garden snail from the Cornu genus, as well as this content in the retail-ready product obtained from these eggs. Material for the study consisted of eggs from two subspecies of edible snails: the small (Cornu aspersum aspersum), and large (Cornu aspersum maxima) common garden snails. The eggs studied were in two forms, the first of which had undergone initial processing to the half-product stage and the second of which was the final product available on the Polish market under the name “Snail Eggs”. The gas chromatography method was used to determine the content of fatty acids. More than 75% of the studied fats were saturated fatty acids, dominated by palmitic and stearic acids. The average content of polyunsaturated fatty acids was 0.37%, and it was a combination of two acids: linoleic (C18:2n6c), and its trans isomer (C18:2n6t). No significant differences were found comparing individual fatty acids content between the two species’ eggs as half-products, or between the half-products and the final product. The fat in raw and processed eggs of common garden snails holds low nutritional value, and the processing did not affect the content of fatty acids.
Mostrar más [+] Menos [-]Snail eggs as a raw material for the production of a caviar substitute Texto completo
2020
Maćkowiak-Dryka Monika | Szkucik Krzysztof | Pyz-Łukasik Renata
Snail eggs as a raw material for the production of a caviar substitute Texto completo
2020
Maćkowiak-Dryka Monika | Szkucik Krzysztof | Pyz-Łukasik Renata
Snail eggs can be the raw material for the production of a caviar substitute. The substitute varies from the original in caloric value and nutrient content which determine the nutritional value of every foodstuff. The present study aimed to determine and compare the nutritional value and protein quality of eggs from two subspecies of edible snail.
Mostrar más [+] Menos [-]Snail eggs as a raw material for the production of a caviar substitute Texto completo
2020
Maćkowiak-Dryka, Monika | Szkucik, Krzysztof | Pyz-Łukasik, Renata
Snail eggs can be the raw material for the production of a caviar substitute. The substitute varies from the original in caloric value and nutrient content which determine the nutritional value of every foodstuff. The present study aimed to determine and compare the nutritional value and protein quality of eggs from two subspecies of edible snail. The chemical composition of the snail eggs i.e. Cornu aspersum maxima and Cornu aspersum aspersum was determined in accordance with international standards. In order to evaluate the protein quality of the eggs of the two studied snail subspecies, the chemical score (CS), and a reference protein were used. Significant differences in the content of water, ash, and carbohydrates, but comparable protein and fat contents and caloric values were found. The protein in the eggs of the snails was complete by the measure of the model adopted for this study, however, meeting the daily essential amino acid requirements of an adult would require an immense supply of both species’ eggs. Snail eggs of the Cornu genus were characterised by much lower nutritional value in comparison with caviar and caviar substitutes.
Mostrar más [+] Menos [-]Effect of goose parvovirus and duck circovirus coinfection in ducks Texto completo
2020
Liu Jie | Yang Xiaoxia | Hao Xiaojing | Feng Yongsheng | Zhang Yuli | Cheng Ziqiang
Effect of goose parvovirus and duck circovirus coinfection in ducks Texto completo
2020
Liu Jie | Yang Xiaoxia | Hao Xiaojing | Feng Yongsheng | Zhang Yuli | Cheng Ziqiang
Coinfection of goose parvovirus (GPV) and duck circovirus (DuCV) occurs commonly in field cases of short beak and dwarfism syndrome (SBDS). However, whether there is synergism between the two viruses in replication and pathogenicity remains undetermined.
Mostrar más [+] Menos [-]Effect of goose parvovirus and duck circovirus coinfection in ducks Texto completo
2020
Liu, Jie | Yang, Xiaoxia | Hao, Xiaojing | Feng, Yongsheng | Zhang, Yuli | Cheng, Ziqiang
Coinfection of goose parvovirus (GPV) and duck circovirus (DuCV) occurs commonly in field cases of short beak and dwarfism syndrome (SBDS). However, whether there is synergism between the two viruses in replication and pathogenicity remains undetermined. We established a coinfection model of GPV and DuCV in Cherry Valley ducks. Tissue samples were examined histopathologically. The viral loads in tissues were detected by qPCR, and the distribution of the virus in tissues was detected by immunohistochemistry (IHC). Coinfection of GPV and DuCV significantly inhibited growth and development of ducks, and caused atrophy and pallor of the immune organs and necrosis of the liver. GPV and DuCV synergistically amplified pathogenicity in coinfected ducks. In the early stage of infection, viral loads of both pathogens in coinfected ducks were significantly lower than those in monoinfected ducks (P < 0.05). With the development of the infection process, GPV and DuCV loads in coinfected ducks were significantly higher than those in monoinfected ducks (P < 0.05). Extended viral distribution in the liver, kidney, duodenum, spleen, and bursa of Fabricius was consistent with the viral load increases in GPV and DuCV coinfected ducks. These results indicate that GPV and DuCV synergistically potentiate their replication and pathogenicity in coinfected ducks.
Mostrar más [+] Menos [-]Occurrence of Marek’s disease in Poland on the basis of diagnostic examination in 2015–2018 Texto completo
2020
Kozdruń Wojciech | Styś-Fijoł Natalia | Czekaj Hanna | Piekarska Karolina | Niczyporuk Jowita Samanta | Stolarek Agnieszka
Occurrence of Marek’s disease in Poland on the basis of diagnostic examination in 2015–2018 Texto completo
2020
Kozdruń Wojciech | Styś-Fijoł Natalia | Czekaj Hanna | Piekarska Karolina | Niczyporuk Jowita Samanta | Stolarek Agnieszka
Marek’s disease (MD) is a tumourous disease caused by Marek’s disease virus (MDV) and most commonly described in poultry. The aim of the study was to determine the occurrence of Marek’s disease virus infections in Poland and analyse clinical cases in the years 2015–2018.
Mostrar más [+] Menos [-]Occurrence of Marek’s disease in Poland on the basis of diagnostic examination in 2015–2018 Texto completo
2020
Marek’s disease (MD) is a tumourous disease caused by Marek’s disease virus (MDV) and most commonly described in poultry. The aim of the study was to determine the occurrence of Marek’s disease virus infections in Poland and analyse clinical cases in the years 2015–2018. The birds for diagnostic examination originated from 71 poultry flocks of various types of production. Birds were subjected to anatomopathological examination post mortem, during which liver and spleen sections and other pathologically changed internal organs were taken. These sections were homogenised with generally accepted methods, then total DNA was isolated and amplified with a real-time PCR. A pair of primers complementary to the MDV genome region encoding the meq gene were used. MDV infection was found predominantly in broiler chicken flocks (69.01%), and also in layer breeder (9.85%) and commercial layer flocks (7.04% each). The results of research conducted in the years 2015–2018 clearly indicate that the problem of MDV infections is still current.
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