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The effect of anise seed powder (Pimpinella anisum) on performance, nutrient digestibility and infectious microbes of suckling calf intestine
2016
Asemi Esfahani, Mahdi | Chaji, Morteza | Eslami, Moosa | Mohammadabadi, Tahereh | Babai, Mehdi
BACKGROUND: Excessive use of antibiotic growth promoters leaded in the creation of antibiotic-resistant bacteria and by consuming animal products, humans are at risk of receiving their residual. Therefore, instead of using antibiotics as growth promoters, some alternatives must be identified and introduced to animal breeders. Objectives: The purpose of this research was to study the effect of anise seed on performance, digestibility, and infectious microbes in the intestine of suckling calves. Methods: Twenty four female Holstein calves with average weight of39.8±3.8kg from the fourth day after birth to two weeks after weaning were examined in a completely randomized design to evaluate the effects of anise powder on dry matter intake, weight gain, age and weight of weaning, feed conversion ratio, apparent digestibility of dry matter, NDF and ADF, and intestinal infectious and harmful bacteria. The diets were: 1- Control, 2- 0.25% and 3- 0.5% anise/DM of diets. Results: Dry matter intake of calves did not affected by diets (p>0.05). At 70 days old the final weight of calves that consumed diets containing 0.25% and 0.5% of anise was significantly more than control diets. There was no significant difference between diets for the mean of total weight gain in whole period of experiment. The digestibility of NDF and ADF after weaning for diets contain anise was significantly more than control diet (p<0.05). Feed conversion ratio did not affected before weaning, but after weaning significantly improved compared with the control group for diet contain 0.25% and 0.5% anise (p<0.05). The age and weight of weaning were not affected by treatments. The Ammonia and pH of rumen fluid showed increase for anise diets compared with the control group (p>0.05). The adding of anise to the diets resulted in significantly decrease of E.coli of intestine of calves. Conclusions: Therefore, use of anise seed as a medicinal herb additive improved the performance of calves. Also, with the decline of E.coli population had beneficial effect on animal health and the environment. So it may be taking into account as good alternative for antibiotics.
Mostrar más [+] Menos [-]Effects of plant-based diets on the bacterial counts and bacterial community composition of beluga sturgeon (Huso huso)
2016
Roohi, Maryam | Agh, Naser | Rezazadbari, Mahmood
BACKGROUND: In recent years, many studies have been conducted on finding a suitable replacement for fish meal to supply the needs of aquaculture feed industry. Objectives: The present study was performed to examine the effect of replacing fish meal and fish oil with plant sources on bacterial counts in intestinal contents and mucus and bacterial composition in Beluga sturgeon. Methods: Beluga sturgeons with a mean initial weight of 133±5 g were distributed into 18 (300 L) tanks (30fish/tank) and were fed experimental diets for 60 days. The control diet contained only fish meal and fish oil as the primary source of protein and lipid, while the experimental diets contained 0, 40, 60, 80 and 100 percent plant protein sources (wheat gluten, corn gluten and soybean meal) and 20% vegetable oil blend (canola, sunflower, cotton and safflower oils) respectively. Results: Results showed that replacement of 80% fish meal and fish oil with plant sources didn’t have significant effect on final weight (235 ± 17 g) compared to control group (256.1 ± 10 g) in a 60 days trial. Replacement of fish meal with 100% plant proteins in combination with 80% vegetable oils resulted in significantly lower final weight (225.7 ± 11 g), compared to other treatments including control group (p<0.05). Replacing 80% fish oil with vegetable oils resulted in a significant reduction of bacterial count in intestinal contents and a significant increase in percentage of Entrobacteriacea sp. However, no significant differences were detected in bacterial counts of mucus in experimental treatments compared to control (p>0.05). Replacement of 60, 80 and 100% fish meal and 80% fish oil with plant sources significantly decreased bacterial counts in intestinal contents and intestinal mucosa compared to control group (p<0.05). Also this replacement cause significant change in bacterial composition of intestine. Conclusions: The results suggested that using plant sources in juvenile Beluga sturgeon diet decreases the bacterial count in the intestinal contents and mucus and changes the composition of intestinal micro flora.
Mostrar más [+] Menos [-]The effect of nanocopper and mannan oligosaccharide supplementation on nutrient digestibility and performance in broiler chickens
2016
Sabet Sarvestany, Shahin | Resvani, Mohammad | Zamiri, Mohammad Javad | Shekarforoush, Shahram | Atashi, Hadi | Mosleh, Najmeh
BACKGROUND: Following the ban on the use of growth stimulant antibiotics in many countries, interest has arisen in finding a suitable alternative for antibiotics in poultry production. OBJECTIVES: The aim of this study was to investigate the effect of supplementation of two levels of nano-copper (0 and 100 mg/kg) and two levels of mannan oligosaccharide (0 or 1 g/kg) as natural growth stimulants on digestibility of nutrients and performance in broiler chicks. METHODS: At the end of the second week of breeding, 160 Cobb-500 broiler chicks were assigned in four treatment groups. For each treatment, four replicates (10 chicks per replicate) were considered. Experiment was conducted as a 2×2 factorial model in a completely randomized design. Results: No significant differences in digestibility of nutrients and growth performance between birds that ate the supplements with control group were observed (p>0.05). CONCLUSIONS: Mannan oligosaccharides and nano-copper on growth performance and nutrient digestibility in broiler chicks had no significant effect. At the doses used, concomitant supplementation of copper and mannan oligosaccharide did not have a positive effect on traits that were studied in broilers.
Mostrar más [+] Menos [-]Comparison of the effects of selected cyclooxygenase enzyme inhibitors on airway responsiveness to histamine and acetyl choline in an experimental model of allergic asthma in guinea pig
2016
Keshavarz, Hossein | Rassouli, Ali | Sadeghi Hashjin, Goudarz | Sassani, Farhang | Moein, Mostafa | Tabarraei, Hadi | Ghaffari, Sepideh
BACKGROUND: Aspirin-induced asthma (AIA) with symptoms such as acute bronchoconstriction occurs in about 10% of asthmatic individuals following ingestion of aspirin or other aspirin-like drugs. The mechanisms involved in AIA are not fully understood but it seems that inhibition of cyclooxygenase (COX) can play a role in such attacks. Objectives: This study aimed to compare the effects of aspirin-like drugs on airway responsiveness in an animal model of allergic asthma. Methods: 42 male guinea pigs (250-300 g) were randomly divided into seven groups: control, allergic, ketoprofen, indomethacin, celecoxib, and aspirin (high and low doses). All animals except control group were sensitized by i.p. injections of ovalbumin and Al (OH)3 suspensions and then challenged by inhalation of ovalbumin solution on day 18. The animals received three oral doses of the aforementioned drugs or normal saline in control and allergic groups with 12 hr intervals within 24 hr before ovalbumin challenge. On day 19, animals were euthanized and the responsiveness of isolated tracheas was studied using cumulative doses of histamine and acetyl choline in an organ bath. Results: Indomethacin and ketoprofen significantly increased the trachea responsiveness to acetyl choline and histamine at all concentrations. Emax values for acetyl choline and histamine in indomethacin were respectively (3.5±0.1, 3.5±0.1 g) and ketoprofen (5.1±0.2, 4.3±0.1) groups were greater than those of control (1.5±0.1, 1.5±0.1), allergic, celecoxib, high dose aspirin and low dose aspirin groups (p<0.05). Conclusions: It was concluded that indomethacin and ketoprofen (but not aspirin and celecoxib) can exacerbate the asthmatic conditions in this animal model.
Mostrar más [+] Menos [-]Evaluation of acute and sub-lethal toxicity of herbicide, Atrazine, on hematological parameters of Tor grypus
2016
Khabazian Zadeh, Ali | Dadolahi Sohrab, Ali | Alishahi, Mojtaba | Khazaei, Seyed Hosein | Mohammad Asgari, Hosein
BACKGROUND: Atrazine is one of the most important and effective pollutants in aquatic ecosystems. The widest sugar cane farms of the Middle East are located in Khouzestan province, Iran. Large amounts of Atrezine are being used in farming on these farms. Objectives: The aims of this study were to investigate acute toxicity (LC50 96 h) of atrazine on Barbus grypus and the effects of chronic toxicity with sub-lethal concentration of atrazine on hematological parameters as well. Methods: 180 B grypus were divided into 4 equal groups (5, 10 and 20% of LC50 96 h and control). Blood samples were taken on days 0, 7, 14 and 21 of experiment. Hematological parameters including hemoglobin level, hematocrit, and red blood cells (RBC), white blood cells (WBC), and globular indices were measured and compared among the groups. Results: LC50 96 h was measured 65 mg l-1. Hematological parameters were significantly affected by chronic toxicity of atrazine. Hemoglobin, hematocrit and RBC were decreased at all sampling periods in fish exposed to different levels of atrazine (p<0.05). There were no significant changes in globular indice values among the groups in sampling periods (p>0.05). For all samples, WBC value was decreased significantly on day 21 (p<0.05). Conclusions: Based on the results, it can be concluded that chronic atrazine toxicity adversely affects hematological parameters of B. grypus.
Mostrar más [+] Menos [-]Effects of Tribulus terrestris extract on excretion of calcium oxalate crystals in hyperoxaluria in cats. an experimental study
2016
Sharifzad, Samieh | Malmasi, Abdolali | Amin, Gholamreza | Bokaie, said | Molazem, Mohammad | Sharifi, Roya
BACKGROUND: Recurrence and side effects of current treatments for urolithiasis confine their application, so other options using traditional herbal therapy are being sought. OBJECTIVES: In this experimental study we have tried to evaluate the antilithiatic effects of herbal extraxt of Tribulus terrestris which is often used to treat different kinds of urinary diseases such as urolithiasis in human on cats. METHODS: To evaluate the therapeutic effect of plant, the hydro alcoholic extract of T.terrestris was assessed for activity against induced urolithiasis in cats. The extract of such herb was administered at daily oral doses of 200 mg/kg for 30 days. RESULTS: Microscopic analysis of urine sediments and ultrasonographic study of kidneys and urinary bladder confirmed inhibition of crystal formation in tested cats. CONCLUSIONS: Our results indicate this herbal extract could be a potential candidate for prevention of urolithiasis in cats. Further studies are needed to clarify the precise anti-crystallization mechanism of T.terrestris in cats.
Mostrar más [+] Menos [-]Evaluation of circulating amino terminal pro-B-type natriuretic peptide concentration in cardiac valvular disease and assessing disease severity in dogs
2016
Tambrchi, Yara | shirani, dariush | masoudifard, majid | khaki, zohreh | chegeni, solmaz | taheri, mohammad
BACKGROUND: One of the most important heart diseases in dogs is chronic valvular disease which can be evaluated by diagnostic ways such as physical examination, electrocardiography, echocardiography and…. Traditionally, the evaluation of heart function has been accomplished by electrocardiography, radiography, and echocardiography. Within the past 15 years, cardiac biomarkers, primarily cardiac troponin and natriuretic peptides, have become a mainstay for both the diagnosis and patient monitoring in human heart disease. Recently, veterinary research has provided insight into the utility of these blood-based cardiac biomarkers in canine and feline patients. OBJECTIVES: The purpose of the current study was to evaluate the chronic valvular disease and assess its severity via evaluation of circulating amino terminal-pro-B-type natriuretic peptide. METHODS: This survey was done on 20 dogs which had valvular disease and on 20 control dogs. Serum NT-pro BNP concentration was measured with an ELISA validated for use in dogs. Results of physical examination, thoracic radiography, echocardiography, and serum biochemical analysis were recorded for dogs with cardiac disease. RESULTS: Serum NT-pro BNP concentration was significantly higher in dogs with cardiac disease than in control dogs. Serum NT-pro BNP concentration in group B2 and C was correlated with left atrial to aortic ratio. CONCLUSIONS: According to this study, results suggested that serum NT-pro BNP concentration could be a useful adjunct clinical test for diagnosing chronic valvular disease and assessing the severity of disease in dogs.
Mostrar más [+] Menos [-]Morphological and molecular characterization of cattle hydatid cysts in Khorramabad, Iran
2016
Kasaeiyan, Fatemeh | Nayebzadeh, Hassan | Jalousian, Fatemeh | Shokrani, Hamid Reza
BACKGROUND: Hydatidosis is one of the zoonotic diseases which affect animals and human beings at the larval stage of Echinococcusgranulosus, thereby playing a role in exacerbating the economic and health problems in Iran. OBJECTIVES: This study was conducted to investigate the strains of Echinococcusgranulosus isolated from cattle hydatid cysts, in Khorramabad, in the Lorestan Province, west of Iran. METHODS: Twenty six isolates of hydatid cyst of cattle from lung (24 samples) and liver (2 sample) organs were collected from Golshan Slaughterhouse, in Khorramabad. All of the samples were transferred to the laboratory for morphometric characterization and molecular study. In morphological characterization, blade length of large (LBL) and small (SBL) hook and the ratio of blade length to total length in large (LBL/LTL) and small (SBL/STL) hooks and total length of large (LTL) and small (STL) hooks were measured. In molecular study, a partial sequence of cytochrome oxidase 1 (COX1) with 440 bp in length was amplified applying primers J3 and J4.5. Genomic DNA sequencing was performed by Sanger’s method. RESULTS: The morphological results showed that there is no significant difference between isolated from cattle hydatid cyst and sensu stricto strain (P<0.05). The results of molecular studies support the findings of morphological characterization. All sequences showed 100% identity with sensu stricto strain. CONCLUSIONS: The results from this study showed that sensu stricto strain (G1) is a causative agent of cattle hydatid cyst in Khorramabad. On the other hand, the cattle play a role in enabling the parasite to complete its cycle. Hence in order to execute a control program for minimizing the effects of this disease, the cattle shall be considered as a source of infection for dogs. The results of this study could be helpful in designing such control program in the region.
Mostrar más [+] Menos [-]Histopathological findings in necrotic spaces developed with doxorubicin and 150 kHz ultrasound at low intensity and a combination of these two methods on adenocarcinoma tumor breast cancer in BALB/c mice
2016
Ghaffari Khaligh, Sahar | tavasoli, abas | Marjanmehr, Seyed Hossein | Soleimani, Homa | Javaheri Vayghan, Abbas
BACKGROUND: Breast cancer is the most commonly diagnosed cancer in women. One in eight women will be diagnosed with breast cancer in their lifetime. Chemotherapy works on active cells. Active cells are cells that are growing and dividing into more of the same type of cell. Cancer cells are active, but so are some healthy cells. Also, scientists work constantly to develop ways of providing treatment with fewer chemotherapy side effects. Objectives: The aim of this study was antitumor effect of simultaneous low-intensity, 150 kHz ultrasound, in combination with the reduced dose of anticancer drug Doxorubicin (DOX) on breast adenocarcinoma using murine model (BALB/c). Methods: Twenty-five female BALB/c mice were used in this study. The tumor was implanted under the breast skin of mice. Mice were divided into five groups, namely control, sham, drug (IV injected of 2 mg/kg of DOX), drug (IV injected of 1 mg/kg of DOX) + US (150 kHz for 15 minutes) and exposure to ultrasound (150 kHz for 15 minutes) alone. The data were analyzed employing ANOVA using SPSS software V.13 and complementary test of Tooki was done. Results: It was shown that, after injection of DOX, exposure to ultrasound at 150 kHz the necrotic spaces in adenocarcinoma tumors compared to control and sham groups have meaningful variance (p<0.001). There was also a significant difference (the bigger the necrotic spaces) between the drug+US group and drug treated group (p<0.05), It should be mentioned that the dose of DOX in drug+US group was reduced to 1mg/kg. Conclusions: The co-administration of DOX and low-intensity ultrasound provided a more effective treatment than the drug alone in murine adenocarcinoma breast cancer. The combined treatment appeared to produce synergistic effects that could prove potentially useful in reducing the side effects of DOX by lowering the required effective dose of the drug while increasing the efficiency of the therapy as a whole.
Mostrar más [+] Menos [-]The effects of Biarum carduchorum and Quercus Infectoria Gall extracts on percentage of hatching and survival rate in the early growth stage of Oncorhynchus mykiss larvae
2016
Akbary, paria | Fereidouni, Mohammad Saeed | Gholam Hosseini, Amin
BACKGROUND: To reduce adverse environmental effects resulting from repeated use of chemical contaminants, herbal extracts are a natural suitable alternative in aquaculture. Objectives: The present study investigated the effects of Biarum carduchorum and Quercus Infectoria Gall extracts on percentage of hatching and survival rate in the early growth stage of rainbow trout (Oncorhynchus mykiss) larvae compared with malachite green (MG). Methods: To assess the percentage of hatching and survival rate in the early growth stage, B. carduchorum was used at 400 mg/l for 30 minutes per day, Q. Infectoria Gall at 40 mg/l for 30 minutes per day and MG as the positive control at 2 mg/l for 20 minutes per day. Normal control without any treatment was also included. Each treatment trial was provided in three replicates. Results: The obtained results showed that the mortality level in normal control was significantly higher than other groups in the hatching stage (p<0.05). Also, the mortality rate in eggs treated with B. carduchorum was significantly lower than both MG and Q. Infectoria Gall groups (p<0.05). The mortality rate in eggs treated with Q. Infectoria Gall was significantly higher than MG group (p<0.05). In addition, the mortality rate of the produced larvae was significantly higher in both Q. Infectoria Gall group and normal control groups than the other two groups (p<0.05).Conclusions: The results showed that use of B. carduchorum at 400 mg/L is able to significantly improve the survival rate of rainbow trout eggs and larvae during incubation period. It can be comparable to MG.
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