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Seroprevalence survey of Chlamydophila abortus infection in breeding goats on commercial farms in the Otavi Veterinary District, northern Namibia
2010
Samkange, Alaster(Ministry of Agriculture Directorate of Veterinary Services) | Katsande, Tendai C.(ARC Onderstepoort Veterinary Institute) | Tjipura-Zaire, Georgina(Central Veterinary Laboratory) | Crafford, Jan E.(University of Pretoria Department of Veterinary Tropical Diseases Faculty of Veterinary Science)
A total of 1076 sera from breeding goats were randomly collected from 24 different farms and tested with CHEKIT®-ELISA (IDEXX Laboratories B.V., 1 119 NE Schiphol-Rijk, Nederland) for antibodies against Chlamydophila abortus. The farms were divided into two categories of twelve farms each, based on their previous history of observed abortions over the previous 12 months: those with low (< 5%) levels of abortion and those with high (> 5%) levels of abortion. The farmers were also interviewed on their level of awareness about chlamydophilosis, its zoonotic importance and vaccination measures against the disease. The study detected overall seroprevalence levels of 25% for the farms and 8% for the individual animals (at 95% confidence). A total of six out of twenty-four farms (25%) had at least one positive breeding animal. Only five out of the twenty-four (20.8%) farmers interviewed were aware of chlamydophilosis and its zoonotic dangers. None of the 24 farmers interviewed practised any vaccination against chlamydophilosis. There was a significantly higher number of seropositive animals from farms with high levels of abortion, compared to those animals from farms with low levels of abortion (p = 0.0001). This study underscores the need for a higher level of farmer awareness and training on chlamydophilosis and its zoonotic dangers.
Mostrar más [+] Menos [-]Comparative ultrastructure of fibrin networks of a dog after thrombotic ischaemic stroke
2010
Pretorius, Etheresia(University of Pretoria Department of Anatomy Faculty of Health Sciences) | Windberger, Ursula B.(Medical University Vienna Center for Biomedical Research) | Oberholzer, Hester M.(University of Pretoria Department of Anatomy Faculty of Health Sciences) | Auer, Roland E. J.(University of Pretoria University of Pretoria Biomedical Research Centre)
A cerebrovascular accident or stroke is a rare condition in dogs, but previous studies suggest that it is now increasingly being recognised. Platelets and fibrin networks are involved in haemostasis, which is disrupted during a thrombotic event. In this study we investigate the ultrastructure of the fibrin networks of a dog that had suffered ischaemic stroke, following suspected thrombo-embolism from clots that became dislodged during catheter maintenance (flushing with heparinised saline) 2 days after carotid artery catheter insertion. Fibrin networks of blood samples that were collected immediately after the stroke, 15 min after treatment with streptokinase and 24 h after treatment, were studied. The results were compared to those of two control dogs. During a stroke, fibrin morphology changes to form a thick, matted layer. Post-treatment ultrastructure shows that the fibrin morphology returns to that comparable to controls. Our results show that during thrombotic risk, fibrin network morphology changes visibly and reduces the fibrinolytic activity of the coagulation system.
Mostrar más [+] Menos [-]Immunomodulating and zootechnical effect of some bacterial components on broiler chicken vaccinated with Newcastle disease vaccine
2010
M. F. El-Kady | S. M. Tamam | Azza A. El Sawah | A. Okasha
This study was carried out to evaluate the immunomodulating effects of, inactivated cells of Propionibacterium acnes and cell wall lipopolysaccharide (LPS) of the a pathogenic E. coli (INMUNAIR® 17.5) 0.5ml/ L and 1-3, 1-6 β-glucans (BETAPOLO®) 1 ml / L on the immune response of chickens to Newcastle disease (ND) vaccine. The results showed that administration of IMR before vaccination was resulted in food conversion rate (FCR) higher than after vaccination . Significantly higher NDV HI antibody titers in IMR and Betapolo medicated groups as compared with control groups which in turn induce high protection rate in challenge test .Thymus, spleen and bursal indices of control negative showed significantly lower values than vaccinated medicated and non- vaccinatedmedicated groups (P≤ 0.05).
Mostrar más [+] Menos [-]Effect of some antioxidants on the reproductive performance in rats
2010
S. S. Ibrahim | A. Aboul-Ela | S. Fathalla | E. A. Mabrouk | A. A. Mohammed
The present study was designed to determine the effect of the antioxidants; taurine, ascorbic acid '' AA '' and beta-mercaptoethanol '' β-ME '' separately or in combination, on reproductive performance of adult male rats suffering from cadmium chloride "CdCl2" - induced oxidative stress. A total of 180 mature male Albino rats were equally divided into 9 groups; the rats of the 1st were administered distilled water while those of the 2nd group were administered 1/100 LD50 of CdCl2. Rats of the remaining groups were administered 1/100 LD50 of CdCl2 followed 3 days later by 1 / 50 LD50 of either taurine, AA and β-ME or a combination of AA and taurine, AA or β-ME, taurine and β-ME as well as AA, taurine and β-ME, respectively for 2 successive months. The results revealed that application of CdCl2 for 8 successive weeks decreased pituitary and serum levels of gonadotropins (gametogenic hormone”FSH” and interstitial cell stimulating hormone “ICSH”) as well as serum testosterone “T” level, altered semen quality, and decreased serum level of total antioxidant capacity with increased serum malondialdhyde "MDA" level. On the other side, application of different antioxidants to CdCl2 –induced oxidative stress increased pituitary and serum levels of gonadotropins and serum level of T as well as improved semen quality, increased serum level of total antioxidant capacity and decreased serum level of MDA. In addition, the best improvement in male reproductive performance was achieved after administration of AA and taurine separately or in combination while the least improvement was obtained when β-ME was applied alone.
Mostrar más [+] Menos [-]Influence of probiotic on serum biochemical profile, growth performance and immunostatus in broiler chickens
2010
M. A. Abonorag | Elham A. Mobarez | Y. A. Elkatan | Sahar A. Galal | Nahla Sh. Kotb
A total of 90 one-day-old male broiler chicks were fed on balanced ration for 15 days and then divided into 3 equal groups. Control group fed ration probiotic free, group 2 and 3 fed ration supplemented with probiotic at concentration levels 0.5 and 1.0g/kg ration respectively for 4 weeks.It was found that probiotic improve body weight gain and feed conversion ratio. Relative organs weights were not altered in groups of birds fed ration mixed with probiotic. Serum values of biochemical parameter (AST, ALT, ALP, total protein, A/G ratio, Ph) were not significantly changed in comparison to control group. Probiotic enhance the immune response of broiler chickens in a dose dependent relation as documented by increasing the serumNDHI antibody geometric mean titres to ND vaccine, phagocyte percentage and phagocytic index of peripheral blood monocyte of broiler chickens. We concluded that probiotic composed of 3 strains of bacteria (Pediococcus acidilactici, Pediococaoccus pentosaceus and Bacillus Amylolique- faciens) in addition to 2 strains of yeast( Pichia farinose and Dekera anomala ) enhance body weight gain, feed conversion, growth performance and Newcastle disease antibody titres in broiler chickens. Moreover, probiotic was safe as proved by serum biochemical profile and relative organ weight of male broiler chickens.
Mostrar más [+] Menos [-]Seroprevalence survey of <i>Chlamydophila abortus</i> infection in breeding goats on commercial farms in the Otavi Veterinary District, northern Namibia
2010
Alaster Samkange | Tendai C. Katsande | Georgina Tjipura-Zaire | Jan E. Crafford
A total of 1 076 sera from breeding goats were randomly collected from 24 different farms and tested with CHEKIT®-ELISA (IDEXX Laboratories B.V., 1 119 NE Schiphol-Rijk, Nederland) for antibodies against Chlamydophila abortus. The farms were divided into two categories of twelve farms each,based on their previous history of observed abortions over the previous 12 months: those with low (< 5%) levels of abortion and those with high (≥ 5%) levels of abortion. The farmers were also interviewed on their level of awareness about chlamydophilosis, its zoonotic importance and vaccination measures against the disease. The study detected overall seroprevalence levels of 25% for the farms and 8% for the individual animals (at 95% confidence). A total of six out of twentyfour farms (25%) had at least one positive breeding animal. Only five out of the twenty-four (20.8%)farmers interviewed were aware of chlamydophilosis and its zoonotic dangers. None of the 24 farmers interviewed practised any vaccination against chlamydophilosis. There was a significantly higher number of seropositive animals from farms with high levels of abortion, compared to those animals from farms with low levels of abortion (p = 0.0001). This study underscores the need for a higher level of farmer awareness and training on chlamydophilosis and its zoonotic dangers.
Mostrar más [+] Menos [-]Incidence of hoof affections in equine
2010
M. M. Seif | A. M. Sedeek | M. Z. Fathy | G. A. Ragab
This study was conducted on 1178 lamed animals of equine species. Cases were classified according to the species, affected part of the foot, and nature of the affection. Hoof affections represented in about 722 animals with 61.3 % of total lame animals. The percentages of hoof affections were higher in forefeet than hind feet. The present study revealed that sandy cracks, toe- in and long toe were the predominant hoof wall affections. The corn was the highest sole affections followed by white line disease and the lowest percentages were flat foot and bruised sole. The highest affection percentage in frog was thrush followed by picked up nail and the lowest was in under run heel.
Mostrar más [+] Menos [-]Hormonal residues in chicken carcasses
2010
F. A. Khalafalla | E. M. El-Neklway | Hala A. M. Ibrahim
Ninety chicks were experimentally, orally received different doses of estradiol-17β and diethylstilbestrol with ration. Samples were collected from tissues (wings, breast muscles, thigh muscles, skin and fat) and giblets (liver and gizzard) for detection of hormonal residues after 4 and 21 days from the last dose, which proved the presence of such residues in all samples. Effect of temperatures (boiling, roasting and freezing) on hormonal residues of positive samples was evaluated. It was proved that There is no significant variations in reduction of hormonal residues in each of breast and thigh muscles of chickens at (p < 0.05) after boiling, roasting and freezing at - 20˚ C as well as a significant differences was detected in skin and fat samples at (p < 0.05) after boiling and roasting. Public health importance of hormonal residues was discussed.
Mostrar más [+] Menos [-]Quality and acceptability of value-added beef burger
2010
F. A. Khalafalla | Fatma H. M. Ali | A. H. Abdel-Azeem | Gehan M. A. Kassem | M. M. T. Emara
The sensory quality attributes of coated and uncoated beef burger patties formulated with texture soy granules or vegetables (peas and carrots) were studied in comparison to that of the control ones. Incorporation of textured soy granules significantly reduced the color, marbling, appearance, flavor, tenderness, juiciness, taste and overall acceptability in comparison with either control or vegetable extended burger. Addition of peas and carrots to uncoated burger significantly reduced the binding scores in raw samples, as well as flavor and juiciness in cooked samples, however, no significant differences could be observed in the other sensory attributes in both raw and cooked products. Vegetable extended burger had the highest cooking loss percent (20.14), followed by control samples (17.83), while soy extended product had the lowest value (15.82%). Application of batter and breading to vegetable extended burger significantly improved the investigated sensory parameters in comparison with the uncoated samples. On the other hand, application of batter and breading to soy extended burger revealed no improvement in the sensory quality attributes in both raw and cooked samples. Addition of soy granules and vegetables significantly increased the moisture, ash and carbohydrate and reduced the fat content of raw burger patties. Moreover the incorporation of textured soy significantly increased the protein content.
Mostrar más [+] Menos [-]Studies on maternal antibodies to avian influenza H9N2 vaccine
2010
M. M. Amer | A. S. Hamouda | K. M. EL-Bayomi
Broiler breeder Lohmann chickens aged 39 weeks received 3 doses each 0.2 ml of the inactivated oil emulsion AI- H9N2 vaccine, at the 2nd, 7th and 15th weeks of age by subcutaneous injection. The individual HI values of the tested samples were homogenous as their SD values were lower. All breeder and progeny sera were positive (100- 66.7%) at weeks 40- 46 weeks of age. Correlation between parents and progeny HI antibody levels was 0.95. Progeny/Parents HI antibodies percentage were ranged from 54.9 to 65.2%. Correlation between parents and progeny ELISA and HI antibody levels were 0.91 and 0.60- 0.65; respectively. The detected HI antibody titres at the 3rd day of age were slightly increased than that of the 1st day titres followed by gradual decrease to be apparently negative at the 12th -21st day of age in comparison to the original levels. The tested groups for Antibodies to H9 by ELISA test were still detected to 21- 27 days of age of progeny. The half-life time of maternal antibodies expressed as loss of one HI log 2 between groups was ranged from 3.3- 7.2 days; with average 5.1- 5.6 days. Half life time by ELISA titre was in average of 8.9 days. Correlations between HI and ELISA ranged from 0.83-0.94. We concluded that both HI and ELISA tests are of the same value in detection of AI antibodies and first vaccination of broiler chicks with maternal antibodies against AI H9N2 must be done after the 6th day of age.
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