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Prevalence of tumours and tumour-like lesions in domestic and exotic animals from Lower Silesia and its surrounds in Poland in 2014–2017 Texto completo
2022
Ciaputa Rafał | Gach Justyn | Baranowski Karol | Dzimira Stanisław | Janus Izabela | Kandefer-Gola Małgorzata | Żebrowski Kacper | Nowak Marcin
An analysis of samples originating from domestic and exotic animals from Lower Silesia but also from other parts of Poland was carried out based on research conducted in 2014–2017.
Mostrar más [+] Menos [-]Two centrifugal flotation techniques for counting gastrointestinal parasite eggs and oocysts in alpaca faeces Texto completo
2022
Maria Pyziel-Serafin Anna | Raboszuk Adrianna | Klich Daniel | Orłowska Blanka | Sierociuk Dagmara | Anusz Krzysztof
The alpaca (Vicugna pacos) is a camelid native to South America, but the species has wide distribution outside its natural habitat and is found in various countries on other continents, Poland being one in Europe. Parasitic infections affect the productivity and health of alpacas. The aim of the study was to estimate the parasite loads in alpacas kept in Poland with the use of two direct centrifugal flotation methods.
Mostrar más [+] Menos [-]The occurrence of Fascioloides magna (Bassi, 1875) in the wild cervid population in the Lower Silesian Wilderness – epidemiological and pathological aspects Texto completo
2022
Filip-Hutsch Katarzyna | Pyziel-Serafin Anna M. | Hutsch Tomasz | Bulak Kamila | Czopowicz Michał | Merta Dorota | Kobielski Janusz | Demiaszkiewicz Aleksander W.
Fascioloides magna is a parasite of high veterinary importance due to its pathogenicity for wild and domestic ruminants. The aim of our study was to describe the presence of trematode infection in the red deer population in the Lower Silesian Wilderness, one of the established fascioloidosis foci in Central Europe, and to assess the overall prevalence of F. magna in the studied area. In order to achieve this, a coprological study of different cervid species was performed.
Mostrar más [+] Menos [-]Effect of serial in vivo passages on the adaptation of H1N1 avian influenza virus to pigs Texto completo
2022
Urbaniak Kinga | Kowalczyk Andrzej | Pomorska-Mól Małgorzata | Kwit Krzysztof | Markowska-Daniel Iwona
Effect of serial in vivo passages on the adaptation of H1N1 avian influenza virus to pigs Texto completo
2022
Urbaniak Kinga | Kowalczyk Andrzej | Pomorska-Mól Małgorzata | Kwit Krzysztof | Markowska-Daniel Iwona
The lack of proofreading activity of the viral polymerase and the segmented nature of the influenza A virus (IAV) genome are responsible for the genetic diversity of IAVs and for their ability to adapt to a new host. We tried to adapt avian IAV (avIAV) to the pig by serial passages in vivo and assessed the occurrence of point mutations and their influence on viral fitness in the pig’s body.
Mostrar más [+] Menos [-]Effect of serial in vivo passages on the adaptation of H1N1 avian influenza virus to pigs Texto completo
2022
Urbaniak, Kinga | Kowalczyk, Andrzej | Pomorska-Mól, Małgorzata | Kwit, Krzysztof | Markowska-Daniel, Iwona
The lack of proofreading activity of the viral polymerase and the segmented nature of the influenza A virus (IAV) genome are responsible for the genetic diversity of IAVs and for their ability to adapt to a new host. We tried to adapt avian IAV (avIAV) to the pig by serial passages in vivo and assessed the occurrence of point mutations and their influence on viral fitness in the pig’s body. A total of 25 in vivo avIAV passages of the A/duck/Bavaria/77 strain were performed by inoculation of 50 piglets, and after predetermined numbers of passages 20 uninoculated piglets were exposed to the virus through contact with inoculated animals. Clinical signs of swine influenza were assessed daily. Nasal swabs and lung tissue were used to detect IAV RNA by real-time RT-PCR and isolates from selected passages were sequenced. Apart from a rise in rectal temperature and a sporadic cough, no typical clinical signs were observed in infected pigs. The original strain required 20 passages to improve its replication ability noticeably. A total of 29 amino-acid substitutions were identified. Eighteen of them were detected in the first sequenced isolate, of which 16 were also in all other analysed strains. Additional mutations were detected with more passages. One substitution, threonine (T) 135 to serine (S) in neuraminidase (NA), was only detected in an IAV isolate from a contact-exposed piglet. Passaging 25 times allowed us to obtain a partially swine-adapted IAV. The improvement in isolate replication ability was most likely related to S654 to glycine (G) substitution in the basic protein (PB) 1 as well as to aspartic acid (D) 701 to asparagine (N) and arginine (R) 477 to G in PB2, glutamic acid (E) 204 to D and G239E in haemagglutinin and T135S in NA.
Mostrar más [+] Menos [-]The potential risk of international spread of Mycobacterium bovis associated with movement of alpacas Texto completo
2022
Krajewska-Wędzina Monika | Miller Michele A. | Didkowska Anna | Kycko Anna | Radulski Łukasz | Lipiec Marek | Weiner Marcin
The study highlights the transboundary nature of tuberculosis (TB) in alpacas and the failure of current ante-mortem testing protocols (the tuberculin skin and Enferplex Camelid TB tests) to identify TB-free alpaca herds and individuals for export. Our research and the available literature indicate that the alpaca (Vicugna pacos) is extremely susceptible to Mycobacterium bovis infection, and that testing periodicity fails to take into account that animals do not manifest disease symptoms for a long time. The skin test failed to identify Mycobacterium bovis infection in two alpacas prior to their movement from the UK to Poland. The animals were purchased by a breeding centre in Poland, and were then shown at an international animal exhibition. The last owner of the alpacas before their deaths from TB bought the infected animals unwittingly in order to run rehabilitation activities with disabled children on his farm.
Mostrar más [+] Menos [-]Animal botulism in Poland – laboratory and epidemiological investigations Texto completo
2022
Grenda Tomasz | Goldsztejn Magdalena | Kwiatek Krzysztof | Kozak Beata | Grenda Anna
Animal botulism in Poland – laboratory and epidemiological investigations Texto completo
2022
Grenda Tomasz | Goldsztejn Magdalena | Kwiatek Krzysztof | Kozak Beata | Grenda Anna
The aim of the study was to present cases of botulism in animals found in Poland in 2019–2021. The analytical laboratory diagnosis and difficulties that occurred in the interpretation of the results are described.
Mostrar más [+] Menos [-]Animal botulism in Poland – laboratory and epidemiological investigations Texto completo
2022
Grenda, Tomasz | Goldsztejn, Magdalena | Kwiatek, Krzysztof | Kozak, Beata | Grenda, Anna
The aim of the study was to present cases of botulism in animals found in Poland in 2019–2021. The analytical laboratory diagnosis and difficulties that occurred in the interpretation of the results are described. From 2019 to 2021, samples of serum, intestinal content, liver, spleen, kidney, faeces, wet feed, dry feed, ensilage, water and mixed samples of internal organs associated with 10 suspected animal botulism cases were sent to the National Veterinary Research Institute. Samples were analysed using a mouse bioassay and culture methods in combination with ntnh and bont gene detection. Among the ten putative botulism cases, only four (40%) were confirmed in the laboratory on the basis of the detection of botulinum toxin (BoNT) or the ntnh or bont genes. The remaining six (60%) were determined as probable despite observable characteristic clinical signs. The diagnosis of botulism in animals is a very difficult task, made so by the heterogeneity of Clostridium botulinum strains and possible loss of toxinogenicity during laboratory processing or the potential degradation of toxins. Laboratory diagnosis is a complex and problematic process which should utilise different prescribed methods for specific types of sample.
Mostrar más [+] Menos [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Texto completo
2022
Ijiri Moe | Odo Kenji | Sato Motohiko | Kawaguchi Maiko | Fujimoto Yoshikazu | Miura Naoki | Matsuo Tomohide | Hou De-Xing | Yamato Osamu | Tanabe Takashi | Kawaguchi Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Texto completo
2022
Ijiri Moe | Odo Kenji | Sato Motohiko | Kawaguchi Maiko | Fujimoto Yoshikazu | Miura Naoki | Matsuo Tomohide | Hou De-Xing | Yamato Osamu | Tanabe Takashi | Kawaguchi Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons.
Mostrar más [+] Menos [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Texto completo
2022
Ijiri, Moe | Odo, Kenji | Sato, Motohiko | Kawaguchi, Maiko | Fujimoto, Yoshikazu | Miura, Naoki | Matsuo, Tomohide | Hou, De-Xing | Yamato, Osamu | Tanabe, Takashi | Kawaguchi, Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons. Pigs were allocated to summer- and winter-finishing cohorts, 12 each. The evaluations included assessment of carcass traits and internal organs’ normality carried out at the time of slaughter, and measurement of biomarkers in whole blood: derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential as markers of oxidative stress, and serum amyloid A and albumin/globulin (A/G) ratio as markers of acute and chronic inflammation, respectively. The summer-finished pigs reared under subtropical field conditions showed lower carcass quality than the winter-finished pigs, indicating a potential adverse effect of summer temperatures on the swine industry. Marginal changes were observed in d-ROMs and the A/G ratio between the summer- and winter-finishing cohorts. The results demonstrate that d-ROMs and the A/G ratio could be used as sensitive markers for heat stress under field conditions.
Mostrar más [+] Menos [-]Proteomic analysis of tear film in canine diabetic patients with and without retinopathy Texto completo
2022
Winiarczyk Dagmara | Winiarczyk Mateusz | Balicki Ireneusz | Szadkowski Mateusz | Michalak Katarzyna | Winiarczyk Stanisław | Adaszek Łukasz
Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers.
Mostrar más [+] Menos [-]Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams Texto completo
2022
Kaba Jarosław | Czopowicz Michał | Witkowski Lucjan | Szaluś-Jordanow Olga | Mickiewicz Marcin | Markowska-Daniel Iwona | Puchała Ryszard | Bagnicka Emilia
Small ruminant lentivirus (SRLV) causes caprine arthritis-encephalitis in goats and maedi-visna disease in sheep. Transmission is via ingestion of colostrum and milk from infected dams or long-term direct contact between animals. Lifelong seroconversion can occur several weeks after infection via ingestion. However, sub-yearling lambs that ingest contaminated colostrum may be able to clear the infection and become seronegative. Whether a similar phenomenon occurs in goats remains unknown. Therefore, the serological status of goats was studied longitudinally from the moment of natural exposure to colostrum and milk of SRLV-positive dams through the age of 24 months.
Mostrar más [+] Menos [-]Effect of immunomodulation in turkeys infected with haemorrhagic enteritis virus on the percentage of CD4+ and CD8α+ T lymphocyte subpopulations synthesising IFN-γ Texto completo
2022
Tykałowski Bartłomiej | Koncicki Andrzej
Haemorrhagic enteritis virus (HEV) is a common turkey pathogen which suppresses the immune function. The immunosuppressive potential of both field and vaccine strains of HEV makes it necessary to seek substances which can limit or prevent this phenomenon. The aim of the presented work was to investigate the effect of two immunomodulators in the immune response of HEV-infected turkeys. The immunomodulators were synthetic methisoprinol and a natural preparation containing 34.2% β-glucans (β-1,3/1,6) and 12% mannan oligosaccharides (MOS).
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