The  breeding  values  of  51  Sahiwal  sires  were estimated from the actual and predicted FL305DMY by applying four sire evaluation methods viz., least squares, simple regressed least squares, best linear unbiased prediction and derivative free restricted maximum likelihood. The derivative free restricted maximum likelihood method had lowest error variance for both actual and predicted first lactation 305-days milk yields and it was considered to be the most efficient method. The BLUP method was second efficient  followed by LSM and SRLS method.

Helminth parasites cause a wide variety of debilitating and frequently fatal diseases in animals. Paramphistomosis caused by the paramphistome Cotylophoron cotylophorum constitutes a major group of disease in domestic ruminants. The anthelmintic activity of Syzygium aromaticum on the motility and acetylcholinesterase (AChE) activity of the digentic trematode Cotylophoron cotylophorum was studied in vitro. The flukes were exposed to various concentrations of hexane, choloroform, ethyl acetate and ethanol extract of Syzygium aromaticum and the motility and mortality of the parasite were observed. As ethanol extract was very effective, further studies were carried out with five different sub lethal concentrations (0.005, 0.01, 0.5, 0.1 and 0.5 mg/ml) of ethanol extract of Syzygium aromaticum (SaEE). The electronic measurement of the motility of the treated parasites clearly indicate the direct impact of the drugs on the motility of the parasite. Maximum inhibition in the motility (86.27%) and AChE activity (86.86%) was observed in 0.5 mg/ml after 8h of exposure. Acetylcholinesterase (AChE) is an enzyme which is involved in neurotransmission. It is present in the cholinergic synapses in the central nervous system as well as in neuromuscular synapses where it rapidly hydrolyzes acetylcholine. Inhibition of AChE in the parasite results in muscular paralysis and the parasite lose its biochemical hold fast and get expelled from the host.

Follicle like structures with a minimum of 17 mm diameter and persisting for more than 6 days in the absence of corpus luteum is called as follicular cyst. It is the hypothalamo-hypophyseo gonadal axis that governs the reproductive events primarily. Plasma is a medium that reflects the changes in any organ apart from providing nutrients and draining the metabolic wastes. Cows brought for infertility treatment were screened and cows which were diagnosed as follicular cyst randomly allotted in to four groups of eight cows each. Different groups were subjected to different treatment protocols viz, Regular co-synch protocol, modified co-synch protocol, Trans-gluteal follicular cyst evacuation technique and trans-gluteal follicular cyst evacuation technique cum modified co-synch protocol for Group I, II, III and IV, respectively. Blood samples were collected from all the cows of different groups on day 0 (initiation of treatment), 7 and 9 of treatment and were analyzed for biochemical parameters. Cholesterol, triglyceride, total protein, calcium and phosphorus concentration was quantified using an automatic analyser UV-VIS spectrophotometer. There was no significant difference (P > 0.05) noticed between the mean concentration of biochemical parameters of different treatment groups, on days 0 and 7 in serum. The mean concentrations of cholesterol were 116.13±20.28, 59.67±10.50, 70.88±6.57 and 64.00±8.36 mg/dl in Group I, II, III and IV cows, respectively and were significantly (P < 0.05) higher in Group I. The mean concentration of all other biochemical parameters were non-significant (P > 0.05) between all treatment groups, on day 9 in serum. This might be due to steroid bio transformation of cholesterol. 

A case was diagnosed as canine multicentric lymphoma based on clinical presentation, FNAC and ultrasonography and was treated with CHOP-19 protocol and remission was observed on 9th week but the protocol was continued up to 19th week and no signs of relapse was noticed. The animal was monitored every month for month after the treatment (19 weeks) animal showed no signs of reoccurrence up to 7 months. Multicentric lymphoma is a disease that the general practitioner can manage; it does not require referral to a specialized practice.

This study was conducted to assess the effect of dietary supplementation of vitamin E and selenium on Japanese quail broilers. This trial was conducted in 525 Japanese quail birds from day- old to five weeks of age. Experimental birds were randomly divided into seven groups viz.T1Control:  Basal diet (standard Japanese quail ration), T2 Basal diet with vitamin E 150mg/kg, T3 Basal diet with vitamin E 300 mg/kg, T4 Basal diet with selenium 0.3mg/kg, T5 Basal diet with selenium 0.6mg/kg,T6 Basal diet with combination of vitamin E 150mg/kg and selenium 0.3mg/kg, T7 Basal diet with combination of vitamin E 300mg/kg and selenium 0.6mg/kg. Dietary supplementation with the combination of vitamin E 300mg/kg and selenium 0.6mg/kg resulted in significantly higher body weight, better feed efficiency, livability percentage and also enrichment of alpha-tocopherol and selenium level of liver, thigh and breast muscles in the Japanese quail broilers.

Ten cattle with severe generalised skin lesions due to dermatophilosis were taken up for the study. Typical exudative dermatitis lesions with matting of hairs, thick scab formation and fissures on the skin were noticed in lower limbs, udder, perineum, inguinal region, axilla, neck and ears. Haematological analysis revealed anaemia and an increase in the absolute eosinophil count in affected animals. Serum biochemical estimations revealed hypoalbuminaemia, hyperglobulinaemia, a decrease in A/G ratio, increase in the blood urea nitrogen value and decrease in the zinc level in affected animals

The study was conducted to find out polymorphism at various growth hormone (bGH) and growth hormone receptor loci (bGHR) by using PCRRFLP technique. Fifty two blood samples of Mehsana buffalo were collected from Livestock Research Station, Sardarkrushinagar Agricultural University, Gujarat. The DNA samples were subjected to PCR amplification using bGH and bGHR specific primers. The PCR products of GH1 (436 bp), GH2 (891bp), GH3 (441bp) and GHR1 (approx 640 bp) loci were digested with Alu 1, Msp 1, Hae III and Mae II restriction enzymes respectively and only AA, CC, FF and RR genotypes were respectively found in Mehsana buffalo. All the bGH and bGHR loci studied were monomorphic.

The giant lizard mantis of Lysiosquilla sulcirostris Kemp, 1913 was recorded for the first time from Thoothukudi waters while investigating the biodiversity of the Gulf of Mannar during September, 2013. One specimen of the said species was collected as an incidental by-catch in the trawl fisheries off Thoothukudi coast of Gulf of Mannar, between 08º 35’ 22.5" N lat. 78º 27’ 40.9" E long and 08º 31’ 91.2"N lat. 78º 25’ 32.7"E long at a depth of 150-160 m.

Milk is the one of the most important protein diet to the human population. However, in last few decades, presence of A1 b casein in milk was associated with important issues associated with range of illnesses in human being. In this study a total of 85 cattle blood samples (Kangeyam and HF crossbred) were analysed for A1 b  casein gene based on AS-PCR. A1/A2 genotype frequency data indicated that 37% were A2 homozygous (A2A2), 17% were A1 homozygous (A1A1) and 46% heterozygous (A1A2) in HF cross bred cattle. The pure Kangeyam (Bos indicus) cattle breed had only A2 gene and showed only A2A2 genotype, which produce safer A2 milk for the human consumption. The Holstein Friesian cross breed animal also showed mostly of A2 gene (0.595). 

An experiment was conducted on 18 un-castrated crossbred (Beetal x Assam local) kids of 3-4 months of age to study the effect of shed roof colour and bedding materials on growth performance during winter season. Three groups of six kids in each were housed in three sheds: T1 (shed roof painted with black colour on top and white colour inside and paddy straw bedding material mixed with hydrated lime, T2 (shed roof painted with black colour on top only and paddy straw without any treatment and T3 (shed roof without any colour and bedding material). The shed T1 and T2 were covered with hessian clothes except the ventilators. The roof shed painted with black colour on top and white colour inside along with hydrated lime mixed bedding material (T1) might have provided better and comfortable micro environment both in terms of optimum temperature and hygienic condition that resulted in significantly (P<0.010) higher body weight in kids (9.59 ± 0.20 kg) than T2 (9.15 ± 0.17 kg) and T3 (8.96 ± 0.0.33kg) respectively.