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Toxicity of lectin extracted from Korean mistletoe (Viscum album coloratum) in chicks and its immunoadjuvant activity on Newcastle disease virus vaccines
2006
Yeo, S.G. (Kyungpook National University, Daegu, Republic of Korea), E-mail: sgyeo@knu.ac.kr
In order to search the availability of the lectin extracted from Korean mistletoe (Viscum album coloratum) as an adjuvant for the avian vaccines, attempts were made to determine toxicity of the lectin in chicks and its immunostimulating activity on the inactivated vaccines against Newcastle disease virus (NDV). For the determination of toxicity, the lectin was injected into the thigh muscle of SPF chicks (Charles River) of 1-week-old and observed hematologically and pathologically.
Mostrar más [+] Menos [-]Toxicity of lectin extracted from Korean mistletoe (Viscum album coloratum) in piglets and its effects on the immunogenicity of Aujeszky's disease virus vaccines
2006
Yeo, S.G. (Kyungpook National University, Daegu, Republic of Korea), E-mail: sgyeo@knu.ac.kr
In the present study toxicity and immunostimulating activity of the lectin (KML-C), which was extracted from Korean mistletoe (Viscum album coloratum) were investigated in swine. To determine the toxicity, lectin was injected into thigh or cervical muscles of 4-week-old piglets (Landrace) and observed clinically and pathologically. For determinatio of the immunostimulating activity, lectin (0.7 ㎍/kg of body weight)-adjuvanted vaccine of Aujeszky's disease virus (ADV) (NYJ1-87) which was inactivated by 0.2% formalin was injected into the cervical muscle of antibody-negative piglets in the same age group.
Mostrar más [+] Menos [-]Effects of formalin on haematology and blood chemistry in crucian carp (Carassius auratus)
2006
Im, C.W. (Chungbuk National University, Cheongju, Republic of Korea) | Park, S.C. (Seoul National University, Seoul, Republic of Korea) | Heo, G.J. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: gjheo@cbu.ac.kr
To determine the effects of exposure to formalin on the secondary stress indices, changes in haematology and blood chemistry were monitored in healthy crucian carps (Carassius auratus). Fishes were separately exposed in a concentration range of 125 to 500 ppm formalin for 60 min. After exposure, red blood cell (RBC) count and packed cell volume (PCV) were elevated in the 500 ppm formalin exposed group. However, mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) were decreased significantly in the 500 ppm formalin exposed group.
Mostrar más [+] Menos [-]Aspiration pneumonia due to grain particle in a tammar wallaby (Macropus eugenii) with Staphylococcus aureus pneumonia
2006
Cho, H.S. (Chonnam National University, Gwangju, Republic of Korea) | Park, N.Y. (Chonnam National University, Gwangju, Republic of Korea), E-mail: nypark@chonnam.ac.kr
A 12-year-old female Tammar Wallaby (Macropus eugenii) died after a 6-day history of depression, anorexia, and coughing. The necropsy revealed pustules of varying sizes on the lung surface from which a pure culture of Staphylococcus aureus was ioslated. Histopathologically, the pulmonary nodules formed typical granulomatous inflammation. The center of the granulomatous foci consisted of a necrotic center and grain particles with gram positive cocci that were surrounded by eosinophilic club-like bodies containing Splendore-Hoeppli material.
Mostrar más [+] Menos [-]Cutaneous melanocytic tumor in a slaughtered pig
2006
Yang, H.S. (Cheju National University, Jeju, Republic of Korea) | Kang, S.C. (Cheju National University, Jeju, Republic of Korea) | Bae, J.H. (Cheju National University, Jeju, Republic of Korea) | Kim, J.H. (Cheju National University, Jeju, Republic of Korea), E-mail: kimjhoon@cheju.ac.kr
A 7-month-old brown pig with a discoid and pedunculate mass measuring 14.0×12.5×2.5 cm on the skin of the right shoulder was noted at the slaughter house in Jeju. The surface of mass approximately 7×4 cm was interfaced with skin. The color of mass with firm consistency was mainly black and partially white on cut surface. Histopathologically, numerous unencapsulated endocrine-like cellular nodules of epithelioid cell type with abundant intracytoplasmic black pigment, melanin, were occupied in dermis and subcutis.
Mostrar más [+] Menos [-]Study on international accreditation for residue analysis laboratory
2006
Kim, M.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: kimmk@nvrqs.go.kr | Cho, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, D.G. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yun, S.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lim, C.M. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Park, S.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, H.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, S.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yun, S.M. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kwon, J.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Son, S.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Chung, G.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, J.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kang, M.I. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Residual materials such as veterinary drugs, environmental contaminants, and pesticides are affecting food safety. High resolution techniques and quality controls are needed to analyze these materials from part per million to part per trillion quantities in food. In order to achieve quality results, standardized methods and techniques are required. Our laboratories were prepared to obtain a certificate of accreditation for ISO/IEC 17025 in the analytical criteria of animal drugs, dioxins, pesticides, and heavy metals. ISO together with IEC has built a strategic partnership with the World Trade Organization with the common goal of promoting a free and fair global trading system.
Mostrar más [+] Menos [-]Effect of ethanol-induced p42/44 MAPkinase activity on IGF system in primary cultured rat hepatocytes
2006
Lee, S.M. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, J.H. (Chonbuk National University, Jeonju, Republic of Korea) | Kang, C.W. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: cwkang@chonbuk.ac.kr
Ethanol abuse is associated with liver injury, neurotoxicity, modulation of immune responses, and increased risk for cancer, whereas moderate ethanol consumption exerts protective effects against liver injury. However, the underlying signal transduction mechanisms of insulin-like growth factors (IGFs) which play an important regulatory role in various metabolism mechanisms are not well understood. We investigated the effects of ethanol-induced p42/44 activity on IGF-I secretion, IGF-I receptor and IGFBP-1 secretion using radioimmunoassay and western blotting in primary cultured rat hepatocytes.
Mostrar más [+] Menos [-]Effects of Suaeda asparagoides M∧IQ extracts on mice ileal motility
2006
Song, J.C. (Kyungpook National University, Daegu, Republic of Korea) | Park, C.H. (Kyungpook National University, Daegu, Republic of Korea) | Kim, H.T. (Kyungpook National University, Daegu, Republic of Korea) | Endale, Mehari (Kyungpook National University, Daegu, Republic of Korea) | Rhee, M.H. (Kyungpook National University, Daegu, Republic of Korea) | Park, S.C. (Kyungpook National University, Daegu, Republic of Korea) | Kim, K.S. (Kyungpook National University, Daegu, Republic of Korea) | Kim, T.W. (Kyungpook National University, Daegu, Republic of Korea), E-mail: twkim@mail.knu.ac.kr
Suaeda (S.) asparagoides M∧IQ, one of the halophyte groups, has been used as a folk remedy for digestive disturbances in Korea. However, its pharmacological activity on gastrointestinal motility has not been reported yet. In this study, the effects of this halophyte extracts with various solvent fractions (ethanol, hexane, chloroform, ethyl acetate, butanol, and water) on mice ileal spontaneous motility was examined. All solvent fractions at the concentration of 100 ㎍/ml showed inhibitory actions on spontaneous motility of ileum with the potency order of water greater-than 70% ethanol greater-than hexane much greater-than chloroform greater-than or equal to butanol greater-than or equal to ethyl acetate, respectively.
Mostrar más [+] Menos [-]Establishment of ethanol-pretreating animal model to study Helicobacter pylori infection
2006
Lee, J.U. (Seoul National University, Seoul, Republic of Korea) | Kim, S.H. (Seoul National University, Seoul, Republic of Korea) | Park, T.W. (Wonkwang University, Iksan, Republic of Korea) | Kim, O.J. (Wonkwang University, Iksan, Republic of Korea), E-mail: kimoj@wonkwang.ac.kr
A stable and reliable Helicobacter pylori (H. pylori) infection animal model would be necessary for evaluating vaccine efficacy and helpful for understanding the pathological mechanism of the organism. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury and to establish ethanol-pretreating animal model to study H. pylori infection. Male Mongolian gerbils were used for the study. H. pylori was orally inoculated after 12 h fasting.
Mostrar más [+] Menos [-]Genetic characterization of Shigella flexneri isolated from the diarrheic patients in Seoul region
2006
Seung, H.J. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Kim, M.S. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Oh, Y.H. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Choi, B.H. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Chae, H.S. (Seoul Metropolitan City Research Institute of Public Health and Environment, Seoul, Republic of Korea) | Chu, J.Q. (Chungnam National University, Daejeon, Republic of Korea) | Jun, M.H. (Chungnam National University, Daejeon, Republic of Korea), E-mail: mhjun@cnu.ac.kr
The shigellae are common etiological agents of bacillary dysentery in humans and primates. During four years from 2002 to 2005, 22 strains of Shigella spp. were isolated from the diarrheic patients in Seoul region. All of them were identified as S. flexneri by biochemical tests and serotyping. The prevalence of serotypes were variable by year, but the major serotypes were 2a and 3a. In an antimicrobial susceptibility test, all of the isolates were resistant to streptomycin and tetracycline, and susceptible to amikacin, kanamycin, cefoxitin, and gentamicin.
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