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Pathological changes in natural infection of pheasants with highly pathogenic avian influenza A (H5N8) in Bulgaria
2019
Stoimenov Georgi M. | Goujgoulova Gabriela V. | Nikolov Branimir | Hristov Kalin | Teneva Atanaska
The study of histopathological changes caused by influenza A (H5N8) viral infection in bird species is essential for the understanding of their role in the spread of this highly infectious virus. However, there are few such studies under natural conditions in minor gallinaceous species. This article describes the pathomorphological findings in Colchis pheasants infected naturally with H5N8 during an epizootic outbreak in Bulgaria.
Mostrar más [+] Menos [-]Early detection of active glomerular lesions in dogs and cats using podocin
2019
Szczepankiewicz Barbara | Pasławska Urszula | Nowak Marcin | Bąchor Remigiusz | Czyżewska-Buczyńska Agnieszka | Pasławski Robert | Szewczuk Zbigniew
In veterinary medicine, sensitive and specific markers of the early stages of renal failure still remain to be established. Podocytes could be a promising diagnostic tool in veterinary nephrology, especially in the differentiation of active pathological disease and glomerulopathies. Podocin is one of the robust proteins exploitable in detection of podocyturia. This article presents podocyte detection in urine for diagnostic purposes in veterinary medicine using a variety of methods. We describe the advantages and disadvantages of the immunohistochemical technique currently used, and of scanning microscopy, chromatography, and immunostaining. The identification of podocin-positive cells is a promising diagnostic tool in the detection of the early stages of glomerular basement membrane damage. The detection of renal failure prior to the occurrence of azotaemia is of high clinical importance from the clinical and scientific points of view.
Mostrar más [+] Menos [-]Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey
2019
Karagul Mustafa Sencer | Malal Mehmet Engin | Akar Kadir
The aim of this study was to investigate Q fever seroprevalence in sheep and goats in the Marmara region. Q fever is a zoonotic disease caused by Coxiella burnetii. In ruminants, the disease causes reproductive disorders, premature births and stillbirths.
Mostrar más [+] Menos [-]First description of histopathological lesions associated with a fatal infection of moose (Alces alces) with the liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932
2019
Filip-Hutsch Katarzyna | Hutsch Tomasz | Kolasa Szymon | Demiaszkiewicz Aleksander W.
Parafasciolopsis fasciolaemorpha is a liver fluke typically parasitising moose in Central and Eastern Europe. The aim of our studies was to describe a case of fatal moose parafasciolopsosis, with special emphasis on the histopathological changes caused in the liver tissue by around 10,000 flukes.
Mostrar más [+] Menos [-]Electrochemical reduction of azo dyes mimicking their biotransformation to more toxic products
2019
Pietruk Konrad | Piątkowska Marta | Olejnik Małgorzata
Some azo dyes, including Sudans I–IV and Para Red, are genotoxic and may be biotransformed to cancerogenic aromatic amines. They are banned as food and feed additives, but their presence has been detected in food. Aromatic amines are also considered potentially toxic. Online EC–MS is a promising tool to study the transformation mechanisms of xenobiotics such as azo dyes. The aim of the study was to investigate emulation of how azo dyes are enzymatically transformed to amines with EC–MS.
Mostrar más [+] Menos [-]Breeding profiles at the periparturient stage in captive red foxes (Vulpes vulpes) mating naturally or subjected to artificial insemination in Japan
2019
Yatu Minoru | Sato Mitsuhiro | Kobayashi Jin | Ichijyo Toshihiro | Satoh Hiroshi | Oikawa Toshinori | Sato Shigeru
Introduction: Breeding profiles at the periparturient stage in red foxes which mated naturally or were subjected to artificial insemination were retrospectively surveyed using 130 vixens during their reproductive seasons of 2012–2017 in Japan. Material and Methods: Natural mating vixens were encouraged a maximum of three times with the same male, while artificial insemination was conducted using frozen-thawed semen with the bovine semen extender as a diluent. Results: With natural mating, conception rates after one, two, and three copulations were 55.8%, 68.0%, and 85.7%, respectively, showing a significant difference between the rates for one and three copulations. Conception rates with artificial insemination were 82.4%. Mean gestation periods were between 52.1 and 53.3 days in all groups. Mean litter sizes were 3.7–4.3 cubs with natural mating, and 4.4 cubs with artificial insemination. Although some sporadic and inconsistent changes in litter sizes were noted between primiparous and multiparous groups, these were of doubtful clinical importance. Conclusion: This is the first report from Japan concerning basic breeding events of red fox vixens in captivity.
Mostrar más [+] Menos [-]Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks
2019
Kursa Olimpia | Tomczyk Grzegorz | Sawicka Anna
Introduction:Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance.
Mostrar más [+] Menos [-]Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats
2019
Gołyński Marcin | Metyk Michał | Szkodziak Piotr | Lutnicki Krzysztof | Kalisz Grzegorz | Szczepanik Marcin | Wilkołek Piotr | Dobrowolski Piotr
Introduction: Thyroid hormones play a major role in the regulation of testicular maturation and growth and in the control of Sertoli and Leydig cell functions in adulthood. When naturally occurring, hypothyroidism causes male hypogonadotropic hypogonadism and Sertoli cell function disorders, but when iatrogenic and methimazole-induced its influence on the pituitary-testicular axis function with respect to Sertoli cells is poorly known.
Mostrar más [+] Menos [-]Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status
2019
Domosławska Anna | Zduńczyk Sławomir | Janowski Tomasz
Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. Results: Before administration, the concentrations of Se in blood plasma were low (86.0–165.0 µg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. Conclusion: Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.
Mostrar más [+] Menos [-]Gastrointestinal nematodes of goats: host–parasite relationship differences in breeds at summer mountain pasture in northern Italy
2019
Zanzani, Sergio Aurelio | Gazzonis, Alessia Libera | Olivieri, Emanuela | Villa, Luca | Fraquelli, Cristina | Manfredi, Maria Teresa
The Orobic goat is a hardy breed native to the Orobic Alps (Lombardy, northern Italy). The aim of the study was the assessment of gastrointestinal nematode (GIN) egg excretion in Alpine and Saanen (cosmopolite breeds) and Orobic grazing goats, after a strategic treatment with eprinomectin in late June. Individual faecal samples from a mixed flock of cosmopolite and Orobic goats were collected and analysed by the FLOTAC double technique every three weeks from June to September. Strongylida was the primary GIN infection observed in goats that grazed on Alpine pastures; a strategic treatment with eprinomectin led to a prolonged reduction of egg excretion during the whole study period. Egg excretion was also influenced by breed. Pluriparous Orobic does were able to control reinfection better than the pluriparous cosmopolite does. Regarding Nematodirus sp. eggs per gram of faeces (EPG), the autochthonous Orobic breed presented higher values than the cosmopolite breeds. However, cosmopolite goats presented higher EPG values of Strongyloides papillosus than their Orobic counterparts in August. Further studies on genetic features of local autochthonous goats, such as the Orobic breed, are needed, since they could reveal peculiar characteristics of susceptibility, resistance or resilience to GIN infection, providing genetic resources for selection.
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