Present study was undertaken in order to evaluate the lead contents of various feedstuffs produced in Korea and the effect of the burning of gasoline by automobiles on the lead contents. Ninty-one samples near and 95 far road were collected from 9 regions(Do). All of the samples were washed, dry-ashed and analyzed for lead content using atomic absorption spectrophotometer. The mean values obtained were checked for significance of difference using computer. The lead content in micro g/g of agriculture-byproducts were measured with the following mean values and ranges (in parenthesis). Rice bran:2.07+-1.67 (0-5.00), rice hull : 1.84+-1.18 (0-3.71), rice straw:1.27+-1.09(0-3.62), soybean pod; 4.55+-3.13 (1.13+-10.43), and wheat bran: 1.69+-0.86 (0-3.65). The lead content in micro g/g of pasture plants were measured with the following mean values and ranges. Kudzu:2.71+-1.74 (0.38+-8.75), leed: 2.03+-1.61 (0-4.54), lespedeza:4.73+-5.93 (0-31.25), sagebrush: 4.08+-3.75 (0-12.18), Korean-grass: 2.11+-1.38 (0-5.50), maizestalk: 2.62+-2.35 (0-8.65), clover: 3.83+-2.97 (0-12.19), alfalfa: 7.14+-0.97 (4.46-9.25), and timothy: 5.86+-4.79 (0-9.25). Fourteen samples (22.2%) out of 63 agriculture-byproduct samples and 42 (34.2%) out of 123 pasture plant samples showed lead content of more than 3 micro g/g which are said to be the upper limit value for cattle. No significant difference between the mean lead contents of near road, and those of far road was recorgnized. The regional mean values for all kinds of samples collected in Kyungbuk and Kyungnam were significantly (p0.05, p0.10) higher compared to those in Chungnam and Chejudo. From these results it may be concluded that the buring of gasoline by automobiles did not affect the lead contentes of washed feedstuffs produced in Korea at present

A total of 593 mastitic pathogens was isolated from the clinical mastitic milk of dairy cattle in Kyonggi area from March 1984 to February 1988. The mastitic pathogens were further studied bacteriologically and examined for susceptibility patterns to 10 antibiotics. 173 pathogens were isolated in the first year of studies, 205 pathogens in the second year, 122 pathogens in the third year and 93 pathogens in the last year. The pathogens isolated from clinical mastitis were more in summer than other seasons. Staphylococcus spp (50%) and Streptococcus spp (18%) were the main pathogens in the first year of studies but coliforms (15%) and other bacteria (40%) were the main pathogens in the last year of studies. Coliform mastitis occurred only in summer and autumn. Antibiotic susceptibility patterns of the same genuses from clinical mastitis infections in different herds and in different years varied greatly. Therefore, treatment should be selected on the basis of susceptibility test results

A preliminary survey of Sarcocystis species in the slaughtered cattle in Seoul was undertaken during October through December 1987, using digestion diagnostic technique for the heart muscle specimens digested in 0.5 % trypsin solution. Results indicated that 41.5 % of 159 bovine hearts were infected with Sarcocystis proliferative forms. High frequencies in the exotic dairy cattle (42.2%) and the Korean native cattle (41.7%) were noticed in comparison with low frequency in the cross breed (25.0%). No differences were indicated between the sexes of the host animals, although an age difference was noticed as 48.9 % in cattle older than four years in comparison with 39.0 % and 37.1 % in younger than two years and in two to four years, respectively

The present study was done to demonstrate ADV antigens in frozen and paraffin sections from ADV-infected pigs and cell cultures by using of the IGS method. Tissue specimens from 3 young pigs infected with ADV-phylaxia strain and of 2 healthy pigs were used. Fibroblastic cells originated from pig brain and BHK cells were grown and confluent monolayers were infected with the virus. Two monoclonal antibodies and a specific hyperimmune serum to ADV were used as the source of primary antibodies for both the IGS and immunoperoxidase methods. Application of the IGS method yielded a black fine granular reaction in positive areas, and the results were superior to those obtained using the immuno-peroxidase technique for all cases tested. The IGS method might be useful in the detection of various viral antigens in tissue sections

The present study was carried out to investigate the serum enzyme activities of racehorses. The enzymes investigated were aspartate and alanine aminotranserase (GOT, GPT), gamma-glutamyl transpeptidase(gamma-GTP), Lactic dehydrogenase(LDH), creatine phosphokinase(CPK), and alkaline phosphatase(ALP). Animals used were 30 healthy racehorse(female 17, male 13) average weighing 435kg and were from 2 to 8 years of age. LDH activity was progressively decreased with age and next in the order of GOT and CPK activities. GOT and gamma-GPT activities were not changed with age but ALP activity tended to be decreased with age. Activities of GOT, GPT, gamma-GTP and ALP were higher in female than in male. There was no difference in CPK activity by sex, and no difference was found out among breeds

To determine the prevalence and the morphological identification of two Eimeria species in fecal examinations, a total of 1092 fecal specimens collected during the period of March to December in 1987 was tested by means of flotation technique. The prevalence of two species was revealed as 1.83% and 0.92% in E. bukidnonensis and E. wyomingensis, respectively. The oocysts of E. bukidnonensis measured in range of 41.3 micro m to 49.4 micro m (mean 45.5+-1.7) x 28.8 micro m to 35.3 micro m (mean 32.2 +-1.2), and E. wyomingensis 36.9 micro m to 44.5 micro m (mean 39.8+-1.7) x 26.5 micro m to 29.9 micro m (mean 28.2+-0.9). The oocysts of E. bukidnonensis appeared darker and more robust than those of E. wyomingensis. The striations in the oocyst wall of E. bukidnonensis were more peculiar than in E. wyomingensis. Among the morphological characteristics, the widths could be reliably taken as a differential point of view for two Eimeria species

Xylazine hydrochloride is a widely used analgesic, sedative and muscle relaxant in veterinary clinic. Yohimbine hydrochloride and 4-aminopyridine are known as antagonists of xylazine hydrochloride. This paper investigated whether or not there exists an antagonism of yohimbine hydrochloride and 4-aminopyridine to xylazine hydrochloride. After xylazine hydrochloride administration, the gizzard motility in chicken was instantly inhibited in relaxation state, and this state was prolonged as the dose was increased. After yohimbine hydrochloride administration, the gizzard motility in chicken showed increase of contractile frequency. After 4-aminopyridine administration, the gizzard motility in chicken was gradually recovered next to decrease of contractile amplitude and frequency. After the combination of yohimbine hydrochloride and 4-aminopyridine administration, the gizzard motility in chicken showed increase of amplitude and radical increase of frequency. After xylazine hydrochloride administration, the relaxation time was shortened by yohimbine hydrochloride, 4-aminopyridine and the combination of yohimbine hydrochloride and 4-aminopyridine. In conclusion, the gizzard motility in chicken was inhibited by xylazine hydrochloride, and this effect was antagonized by combination of yohimbine hydrochloride and 4-aminopyridine

The present study was carried out to investigate the estrous behavior and vaginal smear after induction of estus with exogenous hormones in the premature, metestrous and anestrous bitches. In a total of 21 bitches(Mixed breed: 16, Jindo breed: 5), from 10 months to 5 years of age and weighing 8 to 15 kg the change of vaginal smear and the estrous behavior before and after induction of estrus was studied. In the treatment A(They were given the PGF2alpha, estrone, estradiol-17beta, PMSG and HCG) proestrus commenced in 10.16+-1.44(Mean+-SEM) days after treatment. The mean duration of proestrus and estrus was 7.50+-1.44 and 13.50+-3.44 days, respectively. In the treatment B(They were given the PMSG and HCG) proestrus commenced in 5.53+-0.59 days after treatment. The mean duration of proestrus and estrus was 6.60+-0.71 and 14.60+-1.14 days, respectively. All of the 6 bitches in the treatment A showed vulval swelling and vaginal discharge. 14 of the 15 bitches in the treatment B showed vulval swelling and vaginal discharge. However, all of the treatment A and B showed male acceptance. The main change of vaginal smear in proestrus and estrus after induction of estrus was an increase in the proportion of anuclear and superficial cells associated with a decrease in small intermediate and parabasal cells. By the estrous behavior and vaginal smear the estrus was induced in all the premature, metestrous and anestrous bitches

Some hematological and biochemical indices were assayed in Korean native cattle naturally harbouring Fasciola hepatica infection and compared with uninfected controls. Affected animals revealed reduction in total erythrocyte count and hemoglobin content, and increase in total leukocyte count. Infected cattle had significantly lower levels of serum total protein, serum albumin and magnesium. Significantly higher values were obtained for the serum enzymes asparate aminotransferase and alanine aminotransferase

Ixodes signatus nymphs were subjected to the scanning electron microscopy for the observation of the major physiological sense organs including Haller's organ. Funger prints and variable sensory setae were found on the body surface, and especially well developed setae were identified on the article IV and on the anal lobes. A central button-like organelle and numerous small holes were found in the spiracular plate. The Haller's organ was identified at the distal point of the tarsus I and regarded as the major sensory organ, chemoreceptor. Two types of sensory setae were observed, the shorter ones in front of the organ and the longer ones in distal front as well as behind the organ. The fully opened organ was ellipsoidal and a total of seven basiconic sensory pegs rooted deeply and shaped like well arranged hooks were found inside the organ