Di butyl phthalate (DBP) is a most important plasticizer which is suspected as reproductive toxicant and exposure to it has the  potential effect on the human as well as animal reproductive system. The research work was conducted to evaluate the repeated dose toxicity of di butyl phthalate in male wistar rats. The animals were divided in four different groups with 12 male rats in each group.  Group I served as control and was administered corn oil (2 ml/kg body wt.) while groups II, III, and IV were administered DBP orally at  the dose rate of 500, 1000 and 2000 mg/kg respectively for consecutive seven days. Three rats from each group were sacrificed at 24 hrs, 7th day, 14th day and 21st day after initial dosing. There was gradual decrease in WBC count, RBC count, Hb, HCT, MCV, MCH, MCHC, gradual increase in albumin, gradual decrease in total protein, glucose values, gradual decrease in relative weights of the  testes, epididymis, prostate and seminal vesicles as well as increase in liver weight with increase in dose of DBP in group II, III and IV as compared to control group at different intervals of sacrifice. Grossly reductions in the size of testes were observed only in rats that belonged to high dose group IV on 21st day. The plasticizer DBP was found to produce histopathological lesions in male reproductive organs as well as in liver in dose dependent manner after oral administration. The finding suggested that DBP can cause toxicity  lesions in male reproductive organs especially in testes and epididymis at the dose rate of 500 to 2000 mg/kg body weight in wistar rats.

Swiss cheese samples collected aseptically from private dairies were analysed for the presence of Penicillium citrinum and for the efficacy of antimycotic agents against P.enicillium citrinum. The spore suspension of Penicillium citrinumand antimycotic agents like pimaricin and potassium sorbate at specified concentrations were added to cheese and stored at 250 C for 21 days. The keeping quality of cheese was assessed at 0, 7, 14 and 21 days. On statistical analysis, pimaricin at 10 ppm concentration was found to be highly significant (P <0.01) over 5 ppm of pimaricin, 500 and 1000 ppm of potassium sorbate with regard to anti-mycotic effect.

The study of the influence of aqueous and hydro alcoholic extracts of Tinospora cordifolia on human mammary tumor cell line MCF-7 is a common way of discovering potential therapeutics for treating people suffering from hormone-dependent problems and diseases. In the present study, the cells were treated with the extracts at three different doses viz., 200 μg/ml, 400 μg/ ml and 600 μg/ml. Both the extracts produced degenerative changes in the cell in dose-dependent manner with maximum effect being noticed at the dose level of 600 μg/ml. Thus, these findings suggest that the extracts from Tinospora cordifolia have potential for acting as an antiproliferative agent in mammary tumour.

The squat lobster, Munidopsis cylindrophthalma (Alcock, 1894) was recorded for the first time from the Indian main land coast while investigating the biodiversity of the squat lobster of Gulf of Mannar during April, 2013. One specimen of the said species was collected as an incidental by-catch in the deep sea trawl fisheries off Thoothukudi coast of Gulf of Mannar, between 08º 35’ 22.5" N lat. 78º 27’ 40.9" E long and 08º 31’ 91.2" N lat. 78º 25’ 32.7"E long at a depth of 305-310 m. The present records show its extended distribution from Gulf of Mannar to east of Andamans and further to South China Sea. 

The study was conducted to find out polymorphism at various growth hormone (bGH) and growth hormone receptor loci (bGHR) by using PCRRFLP technique. Fifty two blood samples of Mehsana buffalo were collected from Livestock Research Station, Sardarkrushinagar Agricultural University, Gujarat. The DNA samples were subjected to PCR amplification using bGH and bGHR specific primers. The PCR products of GH1 (436 bp), GH2 (891bp), GH3 (441bp) and GHR1 (approx 640 bp) loci were digested with Alu 1, Msp 1, Hae III and Mae II restriction enzymes respectively and only AA, CC, FF and RR genotypes were respectively found in Mehsana buffalo. All the bGH and bGHR loci studied were monomorphic.

A study was conducted in buffaloes suffering with horn affections .The physiological and haematological parameters were within the normal limits in all the horn affections, but the plasma cortisol and C-reactive protein were more than the normal levels in all the horn affections for initial periods. A radiological sign like increased radio-opacity was noticed in the septic horn cases. Microscopic examination of biopsy samples of horn and skin following amputation revealed normal histology except in horn cancer where squamous cell nests and mitotic figures in the squamous cells were observed.

Campylobacter is the commonest reported bacterial cause of infectious intestinal disease. To study the epidemiology of Campylobacter infection among children, 100 stool samples along with patients anamnesis were collected from the clinical diarrheic reported to Enterology unit of Institute for child Health and centre for Children, Egmore, Chennai, Tamil Nadu between May 2013 to June 2013. Samples were subjected to traditional culture and modern PCR techniques, both of which revealed same results of 13% prevalence of Campylobacter sps (8% C.jejuni & 5% C.coli). Analyzing the patients anamnesis to study the epidemiological determinants, sex has a significance (P<0.05). Age and residential area have no significance and summer season and cross contamination could be probable determinants for the infection.

A trial was conducted to assess the influence of different methods of garlic supplementation on the blood profile of crossbred calves. For studying the blood profile, 18 Jersey crossbred calves of both sexes in the age group of 2-3 months were randomly allotted into three treatment groups of 6 calves in each group. Calves in the first group (T1) were supplemented with garlic powder at the dose rate of 250 mg/Kg BW for a period of 150 days in water whereas calves in the second group (T2) were supplemented with 250mg/Kg BW in concentrate feed and the third group (T3) was treated as control. The data on haemogram were collected and analysed as per standard procedures. There was no significant difference between the treatment groups in the values of either haemogram or serum biochemical parameters during experimental period.

Milk is the one of the most important protein diet to the human population. However, in last few decades, presence of A1 b casein in milk was associated with important issues associated with range of illnesses in human being. In this study a total of 85 cattle blood samples (Kangeyam and HF crossbred) were analysed for A1 b  casein gene based on AS-PCR. A1/A2 genotype frequency data indicated that 37% were A2 homozygous (A2A2), 17% were A1 homozygous (A1A1) and 46% heterozygous (A1A2) in HF cross bred cattle. The pure Kangeyam (Bos indicus) cattle breed had only A2 gene and showed only A2A2 genotype, which produce safer A2 milk for the human consumption. The Holstein Friesian cross breed animal also showed mostly of A2 gene (0.595). 

Laminitis is inflammation of sensitive laminae of the foot in horses and donkey. The front hooves are most commonly affected than hind feet. Laminitis can be mechanical or systematic, acute or chronic, unilateral or bilateral or sometimes all four feet were affectedDonkeys appear to be particularly susceptible to laminitis may be due to live in the desert and dry area so their ‘good bacteria’ are even more sensitive to carbohydrate overload than those of ponies and horses there certainly is no lush grass in the desert and dry area. A two year old male donkey walking very tenderly and repeated raising of left hind leg. The affected hind leg foot was cleaned with 1% Kmno4 solution for avoiding secondary bacterial infection Inj. Meloxicam 35mg I/M, in, inj. Strepto penicillin 500mg I/M were given. The affected left hind leg was exposed to infra red light for 5 minutes and hot fomentation was given with hot salt and hot rice bran mixture. Iodine ointment was applied over the swollen coronary band area of left foot.