Attempts of these studies were made to investigate the nonspecific congenital focal accumulation of etcodermal glial cells in the brain of normal piglets. The brain samples were taken from 1-, 10-, 20-, 35-, 45- and 70-day-old piglets from a SPF-pig farm and three model pig farms. Occurrences of neuroglial cell foci(NCF) on the brain were observed with light microscope. Appearance degrees of the congenital NCF on 10 to 16 cross section slides per a piglets brain were tentatively designed on a scale from deg. + to +++ by NCF number: +, less than 20 of NCF number; , 21-40 of NCF number: +++ , more than 41 of NCF number. NCF in the brain were obseved mainly on the cerebrum. Regions of higher frequencies on the cerebrum were ordered as subependymal layers of the lateral ventricles, peripheral regions of lateral ventricles in the white matter and some neuron layers under the molecular layer of the gray matter. But NCF were not observed in the cerebellum, pons, medulla oblongata and spinal cords. On the subependymal layers of the lateral ventricles, NCF were observed in 100% of 27 piglets, and appearance degree of was observed in 10 piglets (37.0%), ++ in 10 piglets (37.0%) and + in 7 piglets (26.0%) of 27 piglets, respectively. On the white matter of the cerebrum, NCF were observed in 25 piglets (92.6%) of 27 piglets, and appearance degree of +++ was observed in 3 piglets (11.1%), in 13 piglets(48.2%), + in 9 piglets (33.3%) and- in 2 piglets (7.4%) of 27 piglets, respectively. On the gray matter of the cerebum, NCF were observed in 21 piglets (77.8%) of 27 piglets, and appearance degree of +++ was not observed, appearance degree of ++ was obseved in 6 piglets (22.2 %), + in 15 piglets (55.6%) and - in 6 piglets (22.2%) of 27 piglets, respectively. NCF tended to be converged appearance on some regions and tended to be decreased markedly from 35th day after birth, and the shapes of NCF were global or oval forms crowded by analogous shaped and stained cells in the empty spaces of the brain substrate or on one side of the blood vessels

For the laboratory diagnosis of Sarcocystis infections especially in domesticated food animals, several antificial digestion techniques were applied for the musculature specimens and several staining techniques wes applied for the bradyzoites of Sarcocystis species isolated. The digestion technique using trypsin (0.5%) and sodium chloride (0.85%) mixed solution was regarded as the most valuable for the detection of asexual stages of Sarcocystis in bovine musculature specimens. Optimal time for digestion was approximately one to four hours. The trypsin digestion technique with Giemsa's stain could be helpful for the detection of Sarcocystis proliferative forms and for the observation of the nucleus of the parasite. A systematic detection was also performed in an autopsy for a bovine carcass naturally infected with Sarcocystis species, and the asexual stages such as metrocytes and bradyzoites were observed in the specific organs, respectively