In this study twenty four isolates of Pasteurella multocida were collected from field out breaks of Haemorrhagic septicaemia (H.S) in cattle and buffalo from various regions of the country from 1991-2001. The isolates were subjected to detailed cultural, biochemical and pathogenicity test in albino mice. The stereotyping was done by indirect haemagglutination test for determination of their capsular antigen. Serologically all the isolates were classified on the basis of their capsular antigen as Carter Type B. All the twenty four isolates produced oxidase, catalase and indole and reduced nitrate. They did not produce urease. All the isolates fermented fructose, glucose, sucrose and sorbitol and mannitol with the production of acid whereas dulcitol, lactose, silicon were found negative. Variable results were found among isolates on the fermentation of arabinose and maltose. It was concluded that all the isolates were serologically and immunologically homologous to H.S. vaccine strain produced in the institute.

Bovine chondrocytes were cultured in monolayers and alginate beads with or without ascorbic acid (Asc) for 16 days. Cell proliferation was examined every 4 days by staining with Hoechst 33258 dye. The gene expression of aggrecan, and collagen type I and II was analyzed at 16 days by reverse transcription and polymerase chain reaction.Cell morphology and the production of extracellular matrix (ECM) were evaluated by cytochemical, immunocytochemical and electron microscopical methods.Cells were continuously cultured in alginate beads with Asc for 2 months, and the cell morphology and ECM were examined. The proliferation of chondrocytes was significantly stimulated with Asc in both monolayers and alginate beads at 16 days. Expression of the collagen type I gene in both cultures was increased, and that of the collagen type II gene in alginate beads was decreased, by Asc. There were no significant cytochemical and immunocytochemical differences between the cultures in alginate beads with or without Asc at 16 days.In alginate beads cultured with Asc for 2 months, proliferating cells were observed mainly at the periphery of the beads, and glycosaminoglycan and collagen type II were found around the cells. These results suggest that Asc stimulated the proliferation of chondrocytes and maintained the chondrogenic properties of the cells in an alginate beads culture.

This study was undertaken to examine the cell cycle characteristics of bovine fetal and adult somatic cells (fetal fibroblasts, adult skin and muscle cells, and cumulus cells) after culture under a variety of conditions ; 1 ) growth to 60- 70% confluency (cycling) , 2) serum starvation, 3) culture to confluency. Cell -cycle phases were determined by flow cytometry with propidium iodide staining enabling the calculation of percentages of cells in GO /G1, S and G2 /M. The majority was in GO /GI regardless of cell type and treatment. Serumstarved or confluent cultures contained higher percentages of cells in GO /G1 (89.5-95.4% ; P <0.05) . Percentages of cells in GO /G1 increased as cell size decreased regardless of the cell type and treatment. In the serum-starved and confluent cultures, about 98% of small cells were in GO /G1. Serum-starved cultures contained higher percentages of small cells (38.5-66.9%) than cycling and confluent cultures regardless of cell type (P < 0.05) . After trypsinization of fetal fibroblasts and adult skin cells that were serum-starved and cultured to confluency, the percentages of cells in GO /G1 increased (P < 0.05) on incubation for 1.5 (95.7-99.5%) or 3 hr (95.9-98.6%). These results verify that serum starvation and culture to confluency are efficient means of synchronizing bovine somatic cells in GO /G1, and indicate that a more efficient synchronization of the cells in GO /G1 can be established by incubation for a limited time period after trypsinization of serum-starved or confluent cells.

In this study ELISA was standardized for detecting antibodies against Hydropericardium Syndrome Virus. Factors like Antigen, Conjugate and incubation time were studied. Standardization was carried out by checker board titration method. Optimum incubation time for colour development was found to be 15 minutes while 50 mug/ml of antigen gave better results than 100 mug/ml. The conjugate dilution of 1:500 was observed to give satisfactory results as determined by regression analysis. Cut off value was determined by adding 2SDs and 3SDs in the mean optical density (OD) values of negative serum samples. It was found that mean OD+2SD (0.0684) resulted in better diagnostic sensitivity) and diagnostic specificity than mean OD+3SDs (0.0741).

This study was conducted to ascertain anatomical variation in different body parts of Desi, Fayoumi, Cross (Rhode Island Red x Fayoumi) and Nick Chick laying hens. A total 16 laying hens of average body weight were selected using random numbers out of 2600 laying hens, slaughtered and eviscerated. It was observed that proportion of neck, ribs, breast, back, wings, thigh and legs out of aggregate weight was highest (51.74 plus minus 2.85) in Fayoumi hens. The proportion of liver, gizzard, heart and spleen combined weight was highest (6.05 plus minus 1.81) in desi hens. The proportion of non edible offals including trachea, lungs, kidneys, blood, feathers, head, crop, proventriculus, intestines, shanks, gizzard waste, skin, tail, testes, ova, oviduct and fat was estimated to be highest i.e. 46.60 plus minus 1.16 in Nick Chick laying hens. The anatomical variation in Desi and Fayoumi chicks was attributable to scavenging habit of these birds. The high proportion of edible offal in commercial Nick Chick hens may be ascribable to higher productive and reproductive traits.

This study was conducted to determine the optimum storage temperature for Rinderpest vaccine prepared on vero cells to know the shelf life of the vaccine. The vials were randomly selected from one batch of the vaccine, titrated and stored at minus 20 degree centigrade (Freezer), 4 degree centigrade (refrigerator) and room temperature. The titre was found to be 105.1 per ml. The vials stored at minus 20 degree centigrade & 4 degree centigrade were subjected to titration after an interval of six months for 3 and 2 years respectively. The vials stored at room temperature were tested after 4 weeks, 8 weeks and 12 weeks. Titration results indicated that the titre of the vaccine vials stored at room temperature decreased by 100.9 101.2 and 101.6 after storage time of 4 weeks, 8 weeks and 12 weeks respectively. The vials stored at 4 degree centigrade maintained their titre for a period of six months but after that the loss in titre was 100.4, 101.0, and 102.4 after storage time of one, one and half and two 2 years respectively. The vaccine vials stored at minus 20 degree centigrade maintained their original titres (initial titre of the vaccine) even after the storage for three years. It is concluded that vero cell adapted Rinderpest virus vaccine can be stored at 4 degree centigrade for a period of six months, however, at 20 degree centigrade it can be stored for three years without any adverse effect on titre.

This study was conducted to examine growth performance of black, dark brown and light brown varieties of Desi Generation-1 chickens obtained from selected Desi parents (originally procured from rural areas of Mianwali and Bhakkar districts) maintained at Poultry Research Institute, Rawalpindi. A total number of 900 day-old Desi chicks 300 each of black, dark brown and light brown varieties were divided into 3 separate groups and further sub-divided into 3 equal replicates. The experimental chicks were maintained on littered floor in 9 separate pens under optimal managemental conditions for a period of 12 weeks. They were fed ad libitum a balanced chick starter ration (from day- old to 8 weeks) and a grower mash (from 9 to 12 weeks). The birds had free access to clean and fresh drinking water. The results showed non- significant difference in growth rate, feed intake and feed conversion efficiency (feed/kg gain), whereas, significantly (P less than 0.05) lower mortality rate was recorded in Desi light brown chicks in comparison to other two varieties. The better growth performance in different varieties of Desi chicks observed during the present study than reported in earlier findings could be due to effect of genetic improvement through selection breeding carried out in the parent Desi flock.

This trial was conducted to study effect of freezing on quality of broiler meat after freezing. Meat samples were taken from two sites such as breast and thigh. The weight, moisture, protein, fat and ash contents were analyzed before and after freezing at -25 degree centigrade for 15 days using methods given in AOAC, (1990). It was observed that protein, fat and ash contents were not changed consequent to freezing. There was loss (P less than 0.05) in weight which can be attributed to drip loss when meat is thawed. This study provided evidence that there is no change in nutrients when meat is subjected to freezing.