The blood is a connective tissue that composes of suspended cells in liquidmatrix. In this study ,we discuss the effect of three factors on blood sampling duringthe collection of blood samples (anti-coagulants, temperature and duration of storage). The delay of storage processing led to hemolysis of blood sample , increase ofhemoglobin, decrease of red cells ,triglycerides, hematocrite, mean corpuscularvolume, mean corpuscular hemoglobin and its concentration and increase number ofwhite blood cells. The histological analysis of blood sample during long-term storageshowed a slight difference in the shape and size of RBC,WBC and platelets as well ascondensed nucleus bluish cytoplasm and basophilic segmented nucleus in neutrophilsin addition to decrease in sodium ,calcium and chloride. The effect of temperature onblood sampling showed slight variation in shape and size of RBC , basophilicneutrophils and platelets aggregation and decrease of leukocyte number and K levels .

This study was conducted to investigate the potential effect of white, red andgreen color light and two light intensity 5 and 10 lux to each color treatment light onJapanese quail bird’s production performance and some physiological traits. Onehundred and eighty, one day old Japanese quail birds were randomly assigned intothree color light with 5 and 10 lux light intensity per treatment each treatment contain3 replicate (10 birds /cage). Result showed that the birds reared under the influence ofgreen color were significantly (p<0.05) improve body weight, weight gain, feedconversion ratio, relative weight of testes, ovaries, oviduct, male L.H, F.S.H andtestosterone hormones also female L.H, F.S.H and estrogen. Sexual maturity formales and females significantly (p<0.05) increased by white colortreatment.Treatments of birds raised under the influence of 10 lux light intensitysignificantly (p<0.05) increased body weight, weight gain and the average levels ofhormones L.H, F.S.H, testosterone for males and L.H, F.S.H, estrogen for females.Green color light with 5 and 10 lux intensity color significantly (p<0.05) increased therelative weight of ovary and oviduct.

Babesia bigemina is considered as one of the most remarkable blood protozoa in cattleand mainly transmitted via arthropod. This study was conducted on a random group of cows,they numbered 180 local cows who ranged in age from 3-7 years old, from different localities inMosul city north Iraq, comprising both clinically healthy (n=162) and clinically suspectedinfected animals (n= 18). In this study, indirect-enzyme immunosorbent Assay (I-ELISA) wasused to detect of babesia antibodies known as B. bigemina in the blood serum. Then, the bloodand biochemical information that existed from both groups was analyzed, so that the two of themare compared with the control group (n=15). The result showed that the overall seroprevalence ofB. bigemina in cows was 74/180 (41.1%) for clinical and subclinical cows were 10% and 31.1%respectively. The subclinical infected cows was statistically higher than that of clinically infected(P<0.05). Clinically infected cows were suffering from acute onset of the disease includes fever,anorexia, emaciation, drooping in milk yield, jaundice and hemoglobinuria,, with significanthematological and biochemical parameters alterations. While, subclinically infection cowsappeared healthy with absence of changes in blood and biochemistry tests as compared to controlgroups. It has been concluded that significant cases were diagnosed suffering from acuteinfection with the B. bigemina with higher prevalence of subclinical cases in Mosul city, Iraq.

This study was conducted in the veterinary medical college, university of Basra.The objective of this study was to know the histopathological effect of propofol asanesthetic agent on dogs organs (central nervous system, heart, liver) and effect ofpropofol on liver enzymes, Propofol administration for 90 days by intravenous (intocephalic vein) into 8 adult dogs which divided into two equal groups. The controlgroup was injected with 0.9% normal saline (1ml/kg), while the propofol group wasinjected with (10mg /kg) body weight of dog per day. The measured parameters AST,ALT showed a significant difference in groups between zero time and after 90 days.Also the histopathological result of brain, heart and liver showed significant changesas atrophic neurons, nerve fibers vacuolation and gliosis and histopathological resultof heart section showed white areas of degenerate myocardial muscle cells withpresence of adipose tissue and congested blood vessels, white areas of degeneratemyocyte as infiltration of adipose tissue at pericardial region (periphery) and areas ofdestruction of myocardial muscle cells while the histopathological changes ofhepatocyte showed septal fibrosis, bile duct proliferation, fine defuse vacuolation ofhepatocyte and hypertrophic of bile duct. The uses of propofol for the long term maycause serious Histopathological injuries in many organs particularly the brain andliver that may due to its direct interaction in these structural units.

Throughout the period from October 2018 to February 2019, 278 test samples werecollected from animals and human, (55%) animal samples which are distributed to (52.3%)swab samples were from the environment of slaughters and (47.7%) milk samples were fromcow and buffalo which collected in sterile containers. The result showed that Pseudomonaswas found in (44%) samples on pseudomonas agar distributed in (24%) samples fromslaughters, (20%) samples from milk. (45%) human samples that are distributed to (48%)swab samples were from diabetic foot patients and (52%) swab samples were from patientssuffering from burns in hospitals of Basrah province. The results showed that Pseudomonaswas found in (56%) samples on pseudomonas agar, (18%) samples from diabetic foot and(38%) samples from patients suffering from burns. 46 isolates were identified using VITEK 2Kit. 25 samples identified as Pseudomonas aeruginosa which presented (54%).Antimicrobial susceptibility testing of 31 P. aeruginosa isolates compared to 13 differentantibiotics was done by the disk diffusion method. Completely isolates were resistant to as aminimum of 8 antibiotics; they exhibited the form of the multiple resistance to theantibiotics. Thirty-eight samples were tested for 16S rRNA by conventional PCR assay, 19from animal sources and 19 from patients' sources. 18 animals and 19 patient samples weredemonstrated distinct bands with approximately 618bp corresponding for P. aeruginosa.

OBJECTIVE To determine the effects of coadministration of naltrexone, a human opioid abuse deterrent, on the pharmacokinetics and pharmacodynamics of a methadone-fluconazole combination administered orally to dogs. Animals: 12 healthy Beagles. PROCEDURES Dogs (body weight, 10.7 to 13.9 kg) were randomly allocated to 2 groups in a parallel design study. All dogs received fluconazole (100 mg [7.19 to 9.35 mg/kg], PO). Twelve hours later (time 0), dogs were administered methadone (10 mg [0.72 to 0.93 mg/kg]) plus fluconazole (50 mg [3.62 to 4.22 mg/kg]; methadone-fluconazole) or methadone (10 mg [0.72 to 0.93 mg/kg]) plus fluconazole (50 mg [3.60 to 4.67 mg/kg]) and naltrexone (2.5 mg [0.18 to 0.23 mg/kg]; methadone-fluconazole-naltrexone), PO, in a gelatin capsule. Blood samples were collected for pharmacokinetic analysis, and rectal temperature and sedation were assessed to evaluate opioid effects at predetermined times up to 24 hours after treatment. RESULTS Most dogs had slight sedation during the 12 hours after drug administration; 1 dog/group had moderate sedation at 1 time point. Mean rectal temperatures decreased significantly from baseline (immediate pretreatment) values from 2 to ≥ 12 hours and 2 to ≥ 8 hours after methadone-fluconazole and methadone-fluconazole-naltrexone treatment, respectively. Geometric mean maximum observed concentration of methadone in plasma was 35.1 and 33.5 ng/mL and geometric mean terminal half-life was 7.92 and 7.09 hours after methadone-fluconazole and methadone-fluconazole-naltrexone treatment, respectively. Naltrexone was sporadically detected in 1 dog. The active naltrexone metabolite, β-naltrexol, was not detected. The inactive metabolite, naltrexone glucuronide, was detected in all dogs administered methadone-fluconazole-naltrexone. CONCLUSIONS AND CLINICAL RELEVANCE Opioid effects were detected after oral administration of methadone-fluconazole or methadone-fluconazole-naltrexone. Further studies assessing additional opioid effects, including antinociception, are needed.

The objective of this study was to evaluate the effect of intra-abdominal pressure (IAP) on cardiorespiratory parameters during pneumoperitoneum with carbon dioxide in domestic rabbits. Six juvenile female New Zealand white rabbits were assigned to randomized sequences of IAP (0, 4, 8 mmHg) in a crossover study. The following parameters were measured at each IAP: direct arterial blood pressure (ABP); cardiac output, (CO), cardiac index, and stroke volume index (CI, SVI); heart rate; end-tidal carbon dioxide (ETCO(2)); arterial blood gases (PaCO(2), PaO(2)); peak inspiratory pressure (PIP); and peripheral oxygen saturation (SpO(2)). Between IAPs, the abdomen was desufflated for a 5-minute washout period. Mixed linear regression models were used for statistical analysis. Heart rate, SpO(2), and ABP were not significantly affected by IAP, although there was a positive increase in ABP with IAP. Partial pressure of carbon dioxide (PaCO(2)) was increased at an IAP of 8 mmHg and ETCO(2) and PIP were greater with each IAP applied. Cardiac output and CI were significantly decreased with IAP and, although SVI showed the same trend, it was not statistically significant. In conclusion, pneumoperitoneum with carbon dioxide causes an increase in ETCO(2), PaCO(2), and PIP, whereas cardiac output and CI decrease. These cardiorespiratory changes should be considered when determining the optimal IAP for laparoscopic procedures in rabbits.

OBJECTIVE To evaluate the effect of presurgical storage conditions on leakage pressures of enterotomy sites closed with unidirectional barbed suture material in fresh, chilled, and frozen-thawed cadaveric canine jejunal specimens. SAMPLE 36 grossly normal jejunal segments obtained from 4 dog cadavers. PROCEDURES 9 jejunal segments were harvested immediately from each euthanized dog and randomly assigned to be tested within 4 hours after collection (fresh segments), stored at 4°C for 24 hours before testing (chilled segments), or stored at −20°C for 7 days and thawed at 21°C for 6 hours before testing (frozen-thawed segments). For leakage pressure testing, a 3-cm-long antimesenteric enterotomy was performed and repaired with 3-0 unidirectional barbed suture material in a simple continuous pattern in each segment. Time to complete the enterotomy, initial leakage pressure, maximum intraluminal pressure, and leakage location were recorded for each segment. RESULTS Mean ± SD initial leakage pressure for fresh, chilled, and frozen-thawed segments was 52.8 ± 14.9 mm Hg, 51.8 ± 11.9 mm Hg, and 33.3 ± 7.7 mm Hg, respectively. Frozen-thawed segments had significantly lower mean initial leakage pressure, compared with findings for fresh or chilled segments. Time to complete the enterotomy, maximum intraluminal pressure, and leakage location did not differ among groups. CONCLUSIONS AND CLINICAL RELEVANCE Leak pressure testing of cadaveric jejunal segments that are fresh or chilled at 4°C for 24 hours is recommended for enterotomy studies involving barbed suture material in dogs. Freezing and thawing of cadaveric jejunal tissues prior to investigative use is not recommended because leak pressure data may be falsely low.

OBJECTIVE To determine the thermal antinociceptive effects of butorphanol tartrate and butorphanol tartrate in a sustained-release 25% poloxamer 407 (P407) gel formulation (But-P407) in parrots. ANIMALS 13 orange-winged Amazon parrots (Amazona amazonica). PROCEDURES First, butorphanol tartrate (5 mg/kg) or saline (0.9% NaCl) solution was administered IM to birds in a randomized complete crossover design. The temperature prompting a foot withdrawal response to a thermal stimulus (ie, the thermal threshold) was determined 30 minutes before (baseline) and at various points after treatment administration. Second, But-P407 (12.5 mg/kg) or P407 was administered SC in a similar crossover design. Thermal threshold was determined before and at various points after treatment administration. Third, But-P407 (12.5 mg/kg) or saline solution was administered SC and evaluated as in the second trial. Sedation was scored immediately before each time point in all 3 trials. RESULTS In the first trial, a significant increase in thermal threshold was noted 30 minutes after butorphanol tartrate (vs saline solution) administration. No sedation was noted. In the second and third trials, no significant difference was identified between results for But-P407 and those for either control treatment (saline solution or P407). Mild sedation was noted in the second trial following But-P407 administration. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested a small but significant thermal antinociceptive effect of butorphanol tartrate lasting between 30 minutes and 1.5 hours in orange-winged Amazon parrots. No antinociceptive effect of butorphanol tartrate was demonstrated when delivered in P407. Further research is needed to evaluate the potential analgesic effects of But-P407.

OBJECTIVE To evaluate the usefulness of intestinal biomarkers in determining the presence of intestinal epithelial damage in neonatal calves with diarrhea caused by 4 etiologic agents. ANIMALS 40 neonatal calves that were healthy (n = 10) or had diarrhea (30). PROCEDURES The study was a cross-sectional study. Results of hematologic analyses and serum concentrations of intestinal fatty acid–binding protein (I-FABP), liver fatty acid–binding protein (L-FABP), trefoil factor 3 (TFF-3), Claudin-3 (CLDN-3), γ-enteric smooth muscle actin (ACTG2), intestinal alkaline phosphatase (IAP), interleukin-8 (IL-8), platelet-activating factor (PAF), and leptin (LP) were compared among calves grouped according to whether they were healthy (control group; G-1) or had diarrhea caused by K99 Escherichia coli (G-2; n = 10), bovine rota- or coronavirus (G-3; 5 each), or Cryptosporidium spp (G-4; 10). RESULTS Across the 3 time points at which blood samples were obtained and evaluated, the groups of calves with diarrhea generally had markedly higher mean serum concentrations of L-FABP, TFF-3, IAP, IL-8, and LP, compared with the control group. In addition, G-2 also consistently had markedly higher mean serum concentrations of I-FAB and ACTG2 and lower mean serum concentrations of CLDN-3, compared with the control group. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that degree of intestinal epithelial damage differed among calves grouped by the etiologic agent of diarrhea and that such damage might have been more severe in calves with diarrhea caused by K99 E coli. Additionally, our results indicated that serum concentrations of I-FABP, L-FABP, TFF-3, IAP, IL-8, ACTG2, LP, and CLDN-3 were useful biomarkers of intestinal epithelial damage in calves of the present study.