OBJECTIVE To determine the pharmacokinetics of amantadine after oral administration of single and multiple doses to orange-winged Amazon parrots (Amazona amazonica). ANIMALS 12 adult orange-winged Amazon parrots (6 males and 6 females). PROCEDURES A single dose of amantadine was orally administered to 6 birds at 5 mg/kg (n = 2), 10 mg/kg (2), and 20 mg/kg (2) in a preliminary trial. On the basis of the results, a single dose of amantadine (10 mg/kg, PO) was administered to 6 other birds. Two months later, multiple doses of amantadine (5 mg/kg, PO, q 24 h for 7 days) were administered to 8 birds. Heart rate, respiratory rate, behavior, and urofeces were monitored. Plasma concentrations of amantadine were measured via tandem liquid chromatography–mass spectrometry. Pharmacokinetic parameter estimates were determined via noncompartmental analysis. RESULTS Mean ± SD maximum plasma concentration, time to maximum plasma concentration, half-life, and area under the concentration-versus-time curve from the last dose to infinity were 1,174 ± 186 ng/mL, 3.8 ± 1.8 hours, 23.2 ± 2.9 hours, and 38.6 ± 7.4 μg·h/mL, respectively, after a single dose and 1,185 ± 270 ng/mL, 3.0 ± 2.4 hours, 21.5 ± 5.3 hours, and 26.3 ± 5.7 μg·h/mL, respectively, at steady state after multiple doses. No adverse effects were observed. CONCLUSIONS AND CLINICAL RELEVANCE Once-daily oral administration of amantadine at 5 mg/kg to orange-winged Amazon parrots maintained plasma concentrations above those considered to be therapeutic in dogs. Further studies evaluating safety and efficacy of amantadine in orange-winged Amazon parrots are warranted.

OBJECTIVE To determine values of F-wave parameters for the tibial nerve in clinically normal Miniature Dachshunds and those with thoracolumbar intervertebral disk herniation (IVDH). ANIMALS 53 Miniature Dachshunds (10 clinically normal and 43 with various clinical grades of thoracolumbar IVDH). PROCEDURES F-waves were elicited in the interosseous muscles of 1 hind limb in each dog by stimulation of the tibial nerve. F-wave parameters were measured for 32 stimuli/dog, and mean values were calculated. Linear regression was performed to assess correlations between F-wave parameters and clinical severity of IVDH. RESULTS For clinically normal dogs, mean ± SD values of shortest F-wave latency, mean F-wave conduction velocity, mean F-wave duration, and ratio of the mean F-wave amplitude to M response amplitude were 8.6 ± 0.6 milliseconds, 83.7 ± 6.1 m/s, 6.6 ± 1.5 milliseconds, and 9.8 ± 8.5%, respectively. F-wave persistence was 100%. Mean F-wave duration was positively correlated with clinical grade of IVDH. Linear regression yielded the following regression equation: F-wave duration (milliseconds) = 6.0 + 2.7 × IVDH grade. One dog with grade 2 IVDH had a mean F-wave duration shorter than that of all 5 dogs with grade 1 IVDH; 1 dog with grade 3 IVDH had a longer duration than that of all 10 dogs with grade 4 IVDH. CONCLUSIONS AND CLINICAL RELEVANCE Mean F-wave duration was correlated with the severity of inhibitory motor tract dysfunction in the spinal cord of dogs. F-wave examination may be useful for objective functional evaluation of upper motor neurons in the spinal cord.

OBJECTIVE To compare the ability of acetaminophen-codeine (AC; 15.5 to 18.5 mg/kg and 1.6 to 2.0 mg/kg, respectively) or carprofen (4.2 to 4.5 mg/kg) administered PO to attenuate experimentally induced lameness in dogs. ANIMALS 7 purpose-bred dogs. PROCEDURES A blinded crossover study was performed. Dogs were randomly assigned to receive AC or carprofen treatment first and then the alternate treatment a minimum of 21 days later. Synovitis was induced in 1 stifle joint during each treatment by intra-articular injection of sodium urate (SU). Ground reaction forces were assessed, and clinical lameness was scored at baseline (before lameness induction) and 3, 6, 9, 12, 24, 36, and 48 hours after SU injection. Plasma concentrations of acetaminophen, carprofen, codeine, and morphine were measured at various points. Data were compared between and within treatments by repeated-measures ANOVA. RESULTS During AC treatment, dogs had significantly higher lameness scores than during carprofen treatment at 3, 6, and 9 hours after SU injection. Peak vertical force and vertical impulse during AC treatment were significantly lower than values during carprofen treatment at 3, 6, and 9 hours. Plasma concentrations of carprofen (R)- and (S)-enantiomers ranged from 2.5 to 19.2 μg/mL and 4.6 to 25.0 μg/mL, respectively, over a 24-hour period. Plasma acetaminophen concentrations ranged from 0.14 to 4.6 μg/mL and codeine concentrations from 7.0 to 26.8 ng/mL, whereas plasma morphine concentrations ranged from 4.0 to 58.6 ng/mL. CONCLUSIONS AND CLINICAL RELEVANCE Carprofen as administered was more effective than AC at attenuating SU-induced lameness in dogs.

The objectives of this study were to investigate the usefulness of mitral flow propagation velocity (Vp) in cats by evaluating the effect of the flow pattern summation and evaluation of Vp variables in cats with hypertrophic cardiomyopathy (HCM). Healthy cats were categorized into summation (Sum) and separation (Sepa) groups to evaluate the effects of the flow pattern summation on Vp. Cats with HCM were categorized into HCM left atrial (LA) (−), LA (+), and LA (++) groups according to the degree of LA enlargement to investigate the feasibility of Vp. There were no significant differences noted in Vp between the Sum and Sepa groups and no significant correlation between Vp and heart rate. Decline of Vp was associated with the degree of LA enlargement. Mitral flow propagation velocity appeared to be clinically feasible in cats and could possibly be useful in the detection of diastolic dysfunctions in cats with HCM.

Cerebrospinal fluid (CSF) changes are significant for antemortem diagnoses of some neurological diseases. The aim of this study was to evaluate if the concentration of L-lactate in CSF could be used to differentiate healthy from encephalitic cattle. Cerebrospinal fluid samples from healthy cattle (n = 10) and from those naturally affected by rabies (n = 15), bovine herpesvirus type 5 meningoencephalitis (n = 16), histophilosis (n = 6), or bacterial encephalitis (n = 4), including 1 case of listeriosis, were collected and analyzed. Physical, biochemical (i.e., protein and glucose), and cellular analyses were performed in fresh samples. L-lactate, electrolytes (sodium, potassium, and chloride), calcium, and magnesium concentrations were measured in CSF samples that were kept frozen. L-lactate concentrations were also measured in plasma. Analysis of variance was used for comparison between groups and receiver operating characteristic analysis was performed considering L-lactate in CSF of healthy versus encephalitic cattle. The CSF L-lactate concentration was significantly higher in cattle with bacterial encephalitis than in healthy cattle; however, it did not differ between viral and bacterial encephalitis. The calcium concentrations were lower in cattle with encephalitis. L-lactate concentration in CSF > 3.6 mmol/L can be accepted as a cut-off value to indicate encephalitis. Thus, L-lactate in CSF is important for the diagnosis of encephalitis in cattle. Despite the small number of cases of bacterial encephalitis, it is suggested that L-lactate was not important for the differentiation between viral and bacterial encephalitis. Additional studies with a greater number of observations are necessary to clarify this, specifically in cases of listeriosis.

Diabetes mellitus is a type of chronic disease that is non-transmitted. Aceh province is the province with the seventh-highest prevalence of diabetes mellitus in Indonesia at 2.4%. People with type 2 diabetes mellitus are at risk of various complications and can cause death. Self-management of diabetes is one of the things that can be done by people with diabetes mellitus in controlling various complications. This study aimed to find out the relationship between diabetes self-management and blood glucose control of type 2 diabetes mellitus patients in Ulee Kareng Subdistrict, Banda Aceh. This type of sampling method in this research was cross-sectional with the number of samples in this study as many as 88 respondents. The study was conducted using the DSMQ questionnaire filling method to assess diabetes self-management level and blood glucose level value when respondents assess blood glucose control. The results of the study obtained are 30.7% of respondents have a good level of diabetes self- management, 44.3% enough, and 25% bad. As for blood glucose control, 65.9% of respondents had uncontrolled blood glucose levels, and 34.1% controlled. The results of the chi-square test obtained a p-value of 0.000 and the spearman correlation test obtained a p-value of 0.000, as well as a correlation coefficient of 0.492. Based on the data analysis, it can be concluded that there is a significant relationship between moderate and unidirectional levels between diabetes self-management and blood glucose control of type 2 diabetes mellitus patients in Ulee Kareng Subdistrict, Banda Aceh.

The negative effect of consuming unhealthy snacks is foodborne illness caused by food and drinks that have been contaminated with germs. This problems need a serius intervention especially education for the school children to develop understanding of the importance of healthy, safe and nutritious snacks. It was reported that 73% of elementary school students have low knowledge and 81% have low attitude towards healthy snacks. Amongst efforts to educate children on healthy snacks is the audio-visual media. This study is conducted to investigate the effectiveness of animated video and power point as learning media towards the perception of healthy snacks on elementary school students in Indonesia. The design is quasi experiment with control group, with the sample of grade 5s' elementary school students. Data were collected using questionnaires developed from health belief model and intervention is given in the form of health education using animated video media. The results show that there are perception differences in susceptibility (P=0.0001), seriousness (P=0.0001), benefits (P=0.0001), and obstacles (P=0.0001) related to healthy snacks before and after education with animated video. Based on that, it is expected to the schools and parents to give attention to the quality of the snacks by educating and providing the children with healthy snack options.

Up until the recent past, zoos served limited function, primarily existing for entertainment value. Today’s zoos, however, are serving many roles, chief among them: species conservation of captive animals. The biggest zoo in Brazil, S?o Paulo Zoological Park Foundation, has among its 2000 animals and many species of wild cats. The presence of domestic cats living freely in zoos is common and can be a source of spreading disease. The aim of this study was to verify the variety and prevalence of parasites found in the feces of felids (feral and wild) living in the S?o Paulo Zoo. The results of this parasitological analysis have been obtained from the laboratory of clinical analysis and correspond to the 4-year period beginning January/2009 and ending December/2012. Eight species of parasites were identified in the feces of captive wild cats and three in the feces of feral cats. For those captives, Toxocara cati (7.95%) had the highest prevalence, followed by Toxascaris leonina (7.58%), Isospora sp. (2.03%), Hymenolepis nana (0.92%), Eimeria sp., Giardia sp. and Blastocystis sp. (0.37% each) and Ascaris sp. (0.18%). Among the feral cats, we found Toxocara cati (59.26%), Giardia sp. (22.22%) and Isospora sp. (11.11%). For the captive group, we also distinguished natives from exotic species, finding native species to be more frequently parasitized than the exotic ones. Key to our findings, though, was the fact that a few parasite species were found among all groups of felids, specifically (Toxocara cati, Giardia sp. and Isospora sp). Further research is needed, however, to confirm that transmission of these parasites is occurring between and among these groups.

The clinical breast self-examination is one of the strategies to prevent breast cancer to women. Previous study was conducting to promote breast self-examination, but the study did not consider about the role of culture on health behavior. The purpose of this quasi-experimental design was to examine the effect of cultural-based breast self-examination (BSE) educational program on BSE self-efficacy among nursing students. The cultural-based BSE educational program was developed based on Banduras Social Cognitive Theory and Islamic Culture. A stratified proportionate random sampling method was employed to recruit nursing students at Public Nursing College, Faculty of Medicine, Syiah Kuala University in Aceh, Indonesia. Seventy-six students who met the inclusion criteria were recruited, 36 students were in the experimental group and 40 students were in the control group. The experimental group received the cultural-based BSE educational program including exploring Islamic mandate on prevention and individual responsibility in health promotion and cultural related beliefs toward BSE, health education, BSE training, and follow-up. The BSE self-efficacy scores were examined after 3 weeks of program implementation. The statistical analysis was conducted using dependent t-test and independent t-test. The results showed, the BSE self- efficacy of the nursing students in the experimental group were significantly higher than before participation ( t (35) =-5.02, p .001). Further, the BSE self-efficacy score in the experimental group was significantly higher than thecontrol group after participation the program (t (74) = 3.49, p .01). The result showed differences of the overall BSE self-efficacy and the subscale: BSE procedural efficacy (p .01), but not significant differences for the subscale: BSE barrier management efficacy (p .05). The cultural-based BSE educational program is effective in enhancing BSE self-efficacy among nursing students. The study suggests that the application of the cultural-based BSE educational program would be extended for other women and other nursing students in different areas.

The research objective was to determine the beef cattle business development strategy in increasing the economic value of farmers in Sukabumi Regency. The research was conducted in Ciracap District, Sukabumi Regency, West Java in 2020. The research used survey methods and interviews with 65 farmers and 9 extension workers from BPP Sukabumi Regency. Primary data and secondary data were analyzed using statistical analysis, economic analysis and Swot analysis descriptively and quantitatively. The results showed that the farmers' average land ownership was 1.57 ha/farmer. The farmer profit is IDR. 250.548.975 /year, the value of R/C is 1.14. The related variable (dependent) which affects farmer profit and business efficiency of beef cattle has an effect on the95% confidence level. Swot analysis of internal and external factors in the development of beef cattle business showed the strengths of the working area of the Sukabumi Regency BPP including available labor, agricultural land area, grazing land, the number of cows being raised and the support of government institutions, private companies and other institutions as well as livestock market opportunities wide open. The weaknesses are limited land resources, business capital, increased feed prices and the price of beef cattle that were controlled by middlemen. It is suggested that strategies to increase beef cattle population and economic value of the farmers are by optimizing the function of empty land as grazing land and forage cultivation. Business cooperation, marketing networks and guaranteed beef cattle prices are pivotal in order to take advantage of relatively unfulfilled market opportunities.