Objective: This study aimed to analyze the compound content of the combined extracts of Langusei fruit (Ficus minahassae L.) and papaya latex (Carica papaya L.) and perform bioassays of the combination of extracts on fleas of the genus Ctenocephalides. Materials and Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) determined the chemical composition of the combined extract. The toxicity of the extract combination was evaluated in vivo on cat flea and dog flea imago separately. The combination of phytochemical screening of Langusei extract and papaya latex contained all the main phytochemical compounds. Results: The LCMS/MS analysis results showed that the combination of Langusei extract and papaya latex contained six compounds. Four compounds were identified: 3-butenyl glucosinolate, erythromycin, aluminum palmitate, and henpentakontilbenzene. Four compounds indicate a new compound. The toxicity of the combined extract was highest for both Ctenocephalides felis and Ctenocephalides canis in the P4 treatment (10%), with an average mortality of 100%, the same as the control mortality of the synthetic insecticide deltamethrin. The best LD50 for C. felis was in the F1 formula (4.003 mg/l), while in C. canis it was shown in the F3 (3.733 mg/l). Conclusion: Based on the results obtained, the combination of Langusei fruit extract and papaya latex can be developed as an ectoparasiticide for fleas of the genus Ctenocephalides. [J Adv Vet Anim Res 2024; 11(3.000): 810-818]

Objective: This study aims to determine the effect of using multivitamins in different estrus synchronization hormone protocols on estrus response, estrus onset, estrus duration, estrus intensity, and pregnancy rate in swamp buffalo. Materials and Methods: This study used 30 post-partum adult buffalo, with three estrus synchronization methods treated: 1. Conventional plus Prostaglandin F2 α (PGF2α-PGF2α + multivitamin)-AI; 2. Co-synch plus Gonadotropin-releasing hormone (GnRH-PGF2α + multivitamin)-AI; 3. Combination of hormone plus (Estrogen-Progesterone-PGF2α + multivitamin)-AI. Research variables include estrus response, estrus onset, estrus duration, estrus intensity, and pregnancy rate. Data were analyzed using the chi-square test using the Statistical Package for the Social Sciences 23.0 program. Results: The results showed that the use of multivitamins in different estrus synchronization hormone protocols resulted in an estrous response reaching 100%. The onset of estrus in the three treatments [Treatment-1 (T1); Treatment-2 (T2); Treatment-3 (T3)] was 25.8; 27.6; 23.9 h, estrus duration: 21.0; 21.6; 21.92 h, estrus intensity: 25.8; 27.6; 32.6 h, and the pregnancy rate for buffalo reaches 80%. Conclusion: Based on the results of this study, the use of multivitamins in different estrus synchronization hormone protocols is effective in optimizing the swamp buffalo's estrus response; the estrus duration is longer, the estrus onset is faster, and the estrus intensity is higher. It can even optimize the increase in swamp buffalo pregnancy rates. [J Adv Vet Anim Res 2024; 11(3.000): 729-737]

Objectives: This study aimed to compare the body weight (BW), milk yield, nutritional status, and profitability of moderate genetic (MG) and high genetic (HG) merit of Holstein crossbred (HC) cows in a tropical region under the existing farming system. Materials and Methods: Data was gathered from 204 nursing cows of MG (n = 99) and HG (n = 105) merit of HC cows throughout a year in the dairy zone Keraniganj, Bangladesh. HC cows of MG and HG merit contained 50.0%–67.7% and 75.0%–87.5% Holstein blood, respectively. Data on genetic merit, BW, lactation stage and number, daily milk yield, feed intake, feed, and milk price were documented. All variables were except genetic merit analyzed using one-way analysis of variance. Results: HC cows of MG and HG merit had 433 and 493 kg BW (p < 0.01), and daily produced 11.99 and 14.06 kg milk (p = 0.07) with having 0.99 and 1.15 feed efficiency (p = 0.06), respectively but dry matter intake did not vary (p > 0.05). HC cows of both genetic merit daily offered surplus metabolizable energy and digestible crude protein through roughage and concentrate than their requirement (p > 0.05). The milk production cost of both genetic merit HC cows was alike (p > 0.05), whereas almost two times more profit was obtained in HG merit HC compared to MG merit HC cows (p < 0.05). Conclusion: HC cows of HG merit showed superior potentiality of milk yield, profit, and feed efficiency, whereas MG merit HC cows revealed inferior feed efficiency and milk yield. [J Adv Vet Anim Res 2024; 11(3.000): 686-692]

Objective: The evaluation of frozen semen quality is an essential aspect in determining male fertility for artificial insemination programs. This study aims to evaluate the characteristics of Friesian Holstein (FH) bull-frozen semen in different classes (proven and unproven) based on protein profiling and molecular evaluation. Materials and Methods: This study used frozen semen straws from FH bulls selected according to criteria for proven (6 individuals) and unproven (6 individuals) bulls produced by the Singosari AI Center (AIC). Sperm motility parameters were assessed using Computer Assisted Semen Analysis (CASA Supervision®, Germany), while sperm viability and abnormality were evaluated through eosin-nigrosin staining under a microscope at 400´ magnifications. The integrity of the sperm plasma membrane was determined using the hypoosmotic swelling test, and acrosome integrity was analyzed using the fluorescein isothiocyanate PNA-propidium iodide staining method. Protamine deficiency was quantified using Chromomycin A3 fluorescence staining, while DNA fragmentation was assessed using the acridine orange technique. Results: The findings demonstrated that there were no statistically significant differences (p > 0.05) in the assessed parameters of frozen semen quality between FH-proven and unproven bulls. Furthermore, in FH-proven bulls, a negative correlation was observed between protamine deficiency and acrosome integrity (r = −0.528) and between protamine deficiency and sperm DNA fragmentation (r = −0.467). The parameters of protamine deficiency in unproven bulls exhibited a positive correlation with sperm progressive motility. Conclusion: The frozen semen quality of FH bulls in different classes (proven and unproven) was found to be equally good. Molecular-based analysis allows for a more accurate determination of semen quality. These findings are significant for bull breeding stations when comprehensively evaluating semen quality. [J Adv Vet Anim Res 2024; 11(3.000): 796-802]

Objective: The purpose of this study was to investigate the effectiveness of surgical excision combined with a single shot of vincristine sulfate for treating transmissible venereal tumor (TVT) in dogs. Materials and Methods: Fifty-two dogs were divided randomly into two groups (n = 26). Dogs in Group I were treated surgically by debulking the tumorous mass, whereas dogs in Group II were subjected to a combination of surgery and a single injection of vincristine sulfate. Results: Female dogs showed a high prevalence of TVT compared to males (67.3%, n = 35 vs. 32.7%, n = 17), respectively. The German shepherd's breed showed a high prevalence of TVT compared to other breeds. There was a positive association between sex and outcomes. Most occurrences of regret in Group II were reported in females (n = 16, 61.5%) compared to male dogs (n = 10, 38.5%). There was also a positive association between breed and outcome. Most occurrences of regret in Group II were reported in German shepherd dogs (n = 16) compared to Group I (n = 7). In Group I, 15 dogs (57.7%) showed a complete regression, and 11 (42.3%) underwent recurrence. However, in Group II, 21 dogs (80.7%) showed a complete regression, and 5 dogs (19.2 %) underwent recurrence. Conclusions: It appears therefore that the simultaneous use of surgery and administration of a single dose of vincristine sulfate could be considered a combination therapy for TVT as it reduces the risk of recurrence and has a reasonable cost. Recognizing potential risk factors associated with TVT in dogs may be helpful in constructing the best preventive measures. [J Adv Vet Anim Res 2024; 11(3.000): 553-559]

Objective: This study aims to synthesize eco-friendly zinc oxide nanoparticles (ZnO NPs) by utilizing Garcinia mangostana leaf extract and assess the characteristics of ZnO NPs produced throughout different calcination temperatures (300°C, 400°C, 500°C, and 600°C). Materials and Methods: An evaluation was conducted to analyze ZnO NPs using an aqueous extract of G. mangostana leaf bioreductor at different calcination temperatures. The analysis involved the use of a particle size analyzer (PSA), a scanning electron microscope (SEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Results: The PSA and SEM indicated that the ZnO NPs had an average particle size ranging from 641.97 nm to 915.94 nm. Furthermore, the nanoparticles were found in both individual nanoforms and agglomerated forms. The EDX study indicated that the primary constituents of the ZnO NPs were zinc and oxygen. Additionally, the XRD examination demonstrated a distinct peak at 2θ = 36.25°, confirming the presence of a crystalline ZnO structure. The crystal size was determined to be between 40.98 nm and 46.92 nm. An FTIR spectroscopic study verified the existence of ZnO vibrations at distinct wavelengths as well as the absorption peak of the -OH functional group within the range of 3330.58 nm–3415.04 nm. Conclusion: The findings suggest that ZnO NPs produced utilizing the aqueous extract of G. mangostana leaves as a bioreductor can be synthesized at a temperature of 300°C, resulting in a lower particle size compared to those generated at 600°C. [J Adv Vet Anim Res 2024; 11(3.000): 573-582]

Nanotechnology (Nano) applications of feed additives can potentially improve feed-substrate efficiency to enhance livestock productivity. The utilization of Nano in feed in ruminants still tends to be under-explored and reviewed, particularly the application of Nano in trace minerals to enhance the reproductive performance and productivity of ruminants such as selenium. Trace minerals are essential for animal well-being and productivity, and the bioavailability of trace minerals is influenced by a complex matrix of interacting variables, including the chemical form of the min¬erals used and those found in the diet, the nature of the food ingested, the total composition of the diet, and the health and nutrition of the livestock. Nanominerals such as selenium (nano-Se) have shown impressive results when used as animal feed supplements in ruminants. Nano-Se can significantly boost wellness and immunity, gastrointestinal system function, microbiota homeo¬stasis, metabolism, and reproductive performance in ruminants. This review aims to present the current knowledge on the technology of nano-Se in ruminants, ranging from the nanomanufac¬turing procedures of nano-Se, the impact of supplementation on the ruminant digestive system, productivity, and reproductive performance in ruminants in some dosages to find the optimized dosage to be provided. [J Adv Vet Anim Res 2024; 11(3.000): 782-795]

Objective: This study aimed to investigate the impact of thioacetamide (TAA) on the structure and function of the testes and assess the therapeutic effects of Curcuma longa (Cl) against TAA-induced toxicity in rats. Materials and Methods: Thirty-two male albino rats weighing 180–200 gm and aged 11–12 weeks were randomly separated into four groups. The control group was given normal saline, the Cl group was orally administered Cl (500 mg/kg/day), the TAA group received intraperitoneal TAA (200 mg/kg body weight, three times/week), and the Cl with TAA group received Cl orally two hours before TAA administration. After 8 weeks, all rats were anesthetized, and body and testis weights were recorded. Morphological and histological assessments as well as biochemical analyses were conducted. Results: The study revealed a significant decrease in both body and testis weights in the TAA group, accompanied by a substantial increase in luteinizing hormone (LH), follicle-stimulating hor¬mone (FSH), and malondialdehyde (MDA) levels. Testosterone (T) and glutathione (GSH) were significantly decreased in the TAA-treated group compared to the control. Conversely, the Cl-treated group exhibited a substantial decrease in LH, FSH, and MDA levels while showing a significant increase in T and GSH. Conclusion: Cl has been found to have a potential therapeutic role in mitigating TAA-induced testicular damage by acting as an antioxidant. This is supported by a significant decrease in oxi¬dative stress markers and supporting hormonal levels. Further research is needed to understand the underlying mechanisms and explore the clinical applicability of Cl in preventing and treating testicular toxicity. [J Adv Vet Anim Res 2024; 11(3.000): 762-771]

Objective: This study aimed to evaluate the prevalence of cranial cruciate ligament rupture (RCCL) and the efficacy of tibial plateau angle (TPA) as a prognostic indicator in small breed dogs diagnosed with RCCL. Materials and Methods: For this study, 53 dogs with RCCL were selected based on their medical data. The Tibial plateau angle (TPA) was calculated by analyzing the radiographic images of the stifle joint region. The dogs were categorized based on their body weight, sex, age, breed, and RCCL. Subsequently, clinical outcomes of dogs were evaluated based on their pre- and postoper¬ative TPAs. Results: The prevalence of RCCL was higher in neutered dogs, especially those neutered before 6 months of age. The preoperative TPAs of all dogs diagnosed with RCCL were lowered with TPLO. Preoperative walking time in the higher pre-operative TPA (>25°) group (13.58 ± 1.53 days) was significantly (p < 0.05) longer than the lower pre-operative TPA (≤25°) group (10.09 ± 0.84). The lower pre-operative TPA (≤ 25°) group showed better prognoses without any more complications than the higher pre-operative TPA (> 25°) group. Furthermore, post-operative walking time in the higher post-operative TPA dogs (>10°) group (18.08 ± 2.22 days) was significantly (p < 0.05) longer than the lower post-operative TPA dogs (≤10°) group (10.20 ± 0.90 days). Likewise, the TPA (≤10°) group showed better prognoses with lowered complications. Conclusion: Therefore, pre- and post-operative TPA plays an important prognostic factor, and post-operative TPA should be kept at [J Adv Vet Anim Res 2024; 11(3.000): 627-636]

Antimicrobials are employed in the control of contagious illnesses in humans and animals and are also utilized as growth enhancers in livestock and poultry. Improper application of antibiotics results in the development of multi-drug-resistant (MDR) bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant S. aureus (VRSA), colistin-resistant, extend¬ed-spectrum beta-lactamase (ESBL)-producing E. coli, and fluoroquinolone-resistant Salmonella. Transmission of MDR bacteria happens among animals, from human to animal, and vice versa, resulting in treatment failure, increased treatment cost, and high morality. In this article, we analyzed the recent publications of the current antimicrobial application practices in livestock and poultry farms and the development of antimicrobial resistant (AMR) bacteria in livestock and poultry and its adverse effects on human and animal health using PubMed, Google Scholar, and Google. Citations from published articles were also analyzed. Several drug-resistant bacteria, including MRSA, VRSA, colistin-resistant strains, ESBL-producing E. coli, and fluoroquinolone-resis¬tant Salmonella, have emerged due to heavy antibiotic application in cattle and poultry, according to the analysis. Transmission happens between people and animals as well as throughout the production chain, which raises the chance of failure of antibiotic therapy and fatality. To stop the proliferation of drug-resistant bacteria, it is important to ensure the proper use of antibiot¬ics in livestock and poultry. Especially in developing nations, strict control and implementation of antimicrobial rules are necessary. To successfully address antimicrobial resistance and lessen dependency on antibiotics, alternative disease management strategies in livestock and poultry must be developed. [J Adv Vet Anim Res 2024; 11(3.000): 675-685]