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CARDIOPROTECTIVE AND ANTILIPIDEMIC ROLE OF OCIMUM BASILICUM SEEDS OIL AND LINUM USITATISSIMUM SEEDS OIL IN ACUTE MYOCARDIAL INFARCTION MALE RABBITS INDUCED BY ISOPROTERENOL Texto completo
2018
Zainab Abbas Hasan | Muna H. AL-Saeed
This study was undertaken to investigate the possible protective effect of Ocimum basilicumseeds oil and Linum usitatissimum seeds oil on heart function, antioxidant and lipid profile test, wheninduced acute myocardial infarction in rabbits by isoproterenol. Thirty-six male rabbits were dividedinto six groups: group (C): control negative, group (ISO): received isoproterenol (control positive),(BP) group: basil seed oil protective group, (FP) group: flaxseed oil protective group, (BT) group:basil seed oil treated group and (FT) group: flaxseed oil treated group. ECG and blood samplestropnine I, antioxidant enzymes and lipid profile were done. The analysis of ECG in rabbits treatedwith isoproterenol showed T wave inversion and an increase in heart rate. While rabbits treated withbasil oil and flaxseed oil restored T wave and heart rate to near normal. The results of the experimentrevealed that administration of basil oil and flaxseed oil in all groups caused a significant decrease(p˂0.05) in cardiac troponin I and lipid profile (TC, TG, LDL, VLDL) and significant increase(p˂0.05) in HDL and antioxidant enzymes Glutathione Peroxidase (GPx) and SuperoxideDismutase (SOD) compare with ISO group. The study concluded the basil oil and flaxseed oil havecardioprotective and ameliorative effects against acute myocardial infarction induced byisoproterenol in experimental animals
Mostrar más [+] Menos [-]STUDY THE ROLE OF TITANIUM DIOXIDE (TIO2) NANOPARTICLES AGAINST THE TOXICITY OF Echinococcus granulosus IN ADULT ALBINO MALE RATS Texto completo
2018
Abeer A. Ali | Shahad Abbas Alattar
The present study was designed to show the role of green Tio2 nanoparticles against toxicityof Echinococcus granulosus. The present study used 16 adult albino male rats that distributedrandomly to following groups (each group consist 5 rats); control group received ad libidium,second group injected with 2,5 X 103 of Echinococcus granulosus protoscolices third groupinjected with protoscolices and treated with 50mg/kg green Tio2 nanoparticles, fourth groupinjected with protoscolices and treated with 100mg/kg green Tio2 nanoparticles. The resultsshow high significant increased (P < 0.05) in levels of Alanine Aminotransferase (ALT),Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP) in group injected withprotoscolices compared with control group. Oxidative stress factor in group injected withprotoscolices show significant increased (P < 0.05) in levels of MDA (malonedialdehyied) andsignificant decreased (P < 0.05) in levels of glutathione (GSH) and catalase compared withcontrol group. While, after used green Tio2 nanoparticles with Echinococcus granulosus, theresults showed non-significant changes (P < 0.05) in liver functions and MDA, GSH and catalasealso showed non-significant changes (P < 0.05) compared with control group. It was concludedthat green Tio2 nanoparticles has been potential role against toxicity of Echinococcus granulosusin male rats.
Mostrar más [+] Menos [-]MOLECULAR CHARACTERIZATION OF Parabronema skrjabini IN BASRAH PROVINCE, IRAQ Texto completo
2018
Sajjad S. Soud | Ghazi Y. Azzal | Suzan A. Al-Azizz
Parabronema skrjabini is a spirurid nematode of the family Habronematidae thatlives in the abomasum of ruminants such as sheep and goats. The purpose of this studywas to investigate the molecular aspects of Parabronema skrjabini in Basrah. Theworms were collected from slaughtered sheep and goats in Basrah slaughter housebetween the period from June, 2016 to January, 2017, with total number of abomasum'sexamined sheep (576) and goat ( 150 ). An internal transcribed spacer 2 ribosomalDNA (ITS2-rDNA) fragment of P. skrjabini was amplified by polymerase chainreaction (PCR) using a pair of specific primers (Para-Ir-R and Para-Ir-F). ITS2-rDNAsequences by using PCR technique assay which based on a 783-bp long sequence ofthe 28S rRNA gene, the total genomic DNA has been extracted by extracting kit withsome modification. ITS2 homology in different isolates was between (81–86%)compared with the sequence data in GenBank. To our knowledge, this is the first studyin Iraq exploring the genetic diversity of Parabronema in sheep and goats.
Mostrar más [+] Menos [-]GROUNDWATER QUALITY FOR DRINKING BY GOLD-STANDARD AT HIGH-ALTITUDE AREA, TAIF, KSA "VISION 2030 G" Texto completo
2018
Sherifa Mostafa M. Sabra | Afaf Bushara M. Ismail
This paper was for Groundwater (GW) quality for drinking by gold-standard (GS) athigh-altitude (HA) area, Taif, KSA "VISION 2030 G", GW samples contained turbidity,resulted high in samples (6 and 10); (0.03 and 0.018). Public health (PH) were had declinedwithin GS. Electric conductivity (EC) were within the optimum value, total dissolved salts(TDS) were had lowest value. Total hardness (TH) were of more than (300–500) mg L−1,Chloride (Cl−) ranged from (18-1759) mg L−1 with 30% and 70% samples. Sulfate (SO42−)ranged from (33-2245) mg L−1 with 90% falling above GS, Nitrates (NO3−) ranged from (0–60) mg L−1 with 80% falling below GS. Bacterial types revealed both Gram-positive andnegative were not in samples (1, 2, 6 and 7), both Gram-positive and negative were presentin samples (5, 8 and 9). The arrangement of colony count were in samples (9, 1, 8, 6, 7 and5), that was ranged the colony from (550-15) / mL. The common bacteria were isolatedincluded Staphylococcus Species (Staph. Spp.), Micrococcus Spp, Escherichia coli (E. coli)and Klebsiella Spp. The conclusion were discharged from the results, that did not agree withGS to the community use. The most examined GW samples for drinking water (DW) did notagree with GS, bacterial quality did not accepted from GS, will affect PH. Therecommendation were directed to "MOH", to follow up GW for DW through GS at HAarea, so could using for human as DW without any harm and did'not affect PH.
Mostrar más [+] Menos [-]A STUDY OF SOME CHEMICAL PROPERTIES OF CANNED FISH PRODUCTS IN LOCAL MARKETS IN BASRAH CITY, IRAQ Texto completo
2018
Concentrations of seven heavy metals (Cadmium, Lead, Zinc, Nickel, Iron, Copper, and Manganese) in five canned fish samples found in the local market Basrah city, Iraq were determined after digestion according to the Association of Official Analytical Chemists methods. Concentrations of metals were measured using the Flame Atomic Absorption Spectrophotometer. The results showed that the range obtained for the elements analyzed in mg/kg (dry weight) were as follows, Cd (0.0027 - 0.0078), Pb (0.0048 - 0.0093), Zn (0.0103 - 0.0205), Ni (0.0122 - 0.0128), Fe (0.0149 - 0.0237), Cu (0.0008 - 0.0034) and Mn (0.0014 - 0.0037). The results indicated that canned fish samples in this study have concentrations well below the permissible FAO/WHO levels for these toxic metals.
Mostrar más [+] Menos [-]ASSAY OF TETRACYCLINE IN PHARMACEUTICAL PREPARATIONS, SPIKED INDUSTRIAL WASTEWATER AND CHICKEN MEAT SAMPLES USING VISIBLE SPECTROPHOTOMETER TECHNIQUE Texto completo
2018
A simple, accurate, and rapid visible spectrophotometric method has been developed for the determination of tetracycline hydrochloride in pure, pharmaceutical preparations and environmental water samples. The method is based on the oxidation of tetracycline hydrochloride by sodium hypochlorite in alkaline medium to form a red colored product with an absorption maximum at 400 nm. Beer’s Law was obeyed in the range of 2-24 µg/ml with molar absorptivity of 1.346×104 L.mol.-1.cm-1. The relative standard deviation of the method was less than 2% and accuracy (average recovery ) was 100 ± 0.85 %. The optimum conditions for all color development are described and the proposed method has been successfully applied for the determination of tetracycline hydrochloride in pharmaceutical preparations, chicken meat, and wastewater samples.
Mostrar más [+] Menos [-]IMPACT OF GINGER POWDER SUPPLEMENTATION IN BROILER DIET ON THE IMMUNE STATUE DEVELOPMENT AND SMALL INTESTINE MORPHOLOGY Texto completo
2018
A study using 300 one-day-old Ross-308 broilers was conducted to assess the effects of dried ginger root powder (Zingiber officinale) addition at percentages (0.2, 0.4 and 0.6) % in the treatments T1, T2 and T3 respectively on growth performance, immune status and small intestine morphology of broiler chickens at different ages. Compare with the control group (T0) without adding ginger powder. The broiler body weight, body weight gain, viability, production index, and economic profit impacted with addition of ginger powder significantly (P≤0.05) increase in T3 at ages (1-21 d) and (36-42). As well as feed conversion ratio (FCR) particularly improved. In addition the morphological of small intestine goblet cells number in (duodenum , jejunum, and ileum), immunological delayed type of hypersensitivity DTH and ELISA titer against Newcastle(ND), Gumboro (IBD) and Infectious bronchitis (IB) diseases of broiler chickens were significantly higher (P≤0.05) at the ages 21, 35 and 42 d. While mortality and H/L ratio were significantly lower in the treatments of ginger supplementation especially in T3 compare with the control group without ginger addition.
Mostrar más [+] Menos [-]HISTOMORPHOLOGICAL POSTNATAL DEVELOPMENTAL STUDY OF THE OVARIES OF THE LOCAL RABBITS (Oryctologus Cuniculus) Texto completo
2018
This investigation was carried out to point out structural histomorphological changes that take place in the ovaries of female local rabbits at three different postnatal periods of their life that were kitten, immature and mature does. To conduct such project, 24 rabbits were collected (8 for each age) from the local breeders. Rabbits were euthanized, dissected and subsequently ovaries were collected and fixed with 10% neutral buffered formalin then subjected to routine processing such as dehydration, clearing, embedding and block preparation. Finally, sections of 6 µm were prepared and stained with hematoxylin-eosin and Masson’s trichrome stains. Gross findings showed two functional bilateral elongated triangular-shaped ovaries situated in the abdominal cavity suspended by mesovarian ligament with absence of true ovarian bursa. Microscopic findings revealed prominent structural changes in the ovaries of immature does compared to those of kittens and mature does indicated that immature does after the period of weaning face great developmental growth and changes. The data showed the presence of pre-ovulatory follicles in the mature does and no signs of ovulation because these animals are induced ovulators and that does subjected to the study were all virgin and not allowed for mating with the males.
Mostrar más [+] Menos [-]EFFECTS OF DRENCHING AQUOUS EXTRACTS OF LICORICE ROOT (Glycyrrizia galabra)AND Oreganum vulgari on HEMATOLOGICAL CHARACTERISTICS OF ADULT MALE RABBITS (Lepus cunicolus). Texto completo
2018
This study was done on thirty mature male rabbits of 1500 gm mean body weight, those rabbits were randomly divided into three equal experimental groups of ten animals each .The first group was regarded as control group and drenched 3 ml normal saline daily for one month. The second group animals were drenched 3ml of licorice root water extract daily for one month also. While the third group animals were drenched 3ml of water extract of oregano daily for one month also. At the end of the drenching period blood samples were drawn from all animals by heart puncture and blood was analyzed for total RBC count; Hb %; pcv%; total leukocytic count and blood indices of MCH; MCV, and MCHC were calculated in addition to doing blood smears from each animal to estimate percentages of differential leukocytic count. Results revealed that drenching licorice water extract had a significant increase (p<0.05) on total RBC count; total WBC count and neutrophils also, significant decrease in MCHC and eosinophil, whereas results of drenching water extract of Oreganum vulgari caused significant increase on total leukocytic count , MCV and neutrophil while RBC had no significant effect so, PCV, Hb, had no effect in the two experimental.
Mostrar más [+] Menos [-]PROTECTIVE EFFECT OF MYRTUS COMMUNIS L.ON ARSENIC-INDUCED PATHOLOGICAL CHANGES ON BRAIN TISSUE OF WHITE RATS. Texto completo
2018
The study was performed on twelve white rats of approximately of the same body weight ( 200-220 gms). It divided equally into four groups. The 1st group was injected with (10mg/kg B.W) arsenic chloride intraperitoneally, 2nd group was injected with (10mg/kg B.W) arsenic chloride intraperitoneally and (3 mg/ml) myrtle extracts. The 3rd group was normal to control, rats treated orally with(3 mg/ml) myrtle extract only .4th group was injected with (0.2ml) distal water as a control group. At the end of the experiment, the animals were sacrificed, and small specimens(2cm³) were taken from brain to histopathology preparation. The microscopic examination of histopathological sections of the brain of the the1st group was showed severe pathological changes characterized by an area of degeneration, vacuolation of microglia &pyknosis of a neuron also there are edema&hemorrhage.On the other hand, the 2ndgroup showed less pathological changes compared by the 1st group which characterized by less gliosis, less vaculation of microglia with few numbers of astrocyte. The neuron showed normal. while, the 3rd and 4th groups showed normal neurons, microglial, and astrocyte
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