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Studies on pregnancy diagnosis in domestic animals by an ultrasonic Doppler method, 1: Pregnancy diagnosis in the pig and fetal heart rate changes during pregnancy.
1974
Too K. | Kawata K. | Fukui Y. | Sato K. | Kagota K. | Kawabe K.
Intestinal absorption of D-xylose and folic acid measured by a perfusion technique in patas monkeys (Erythrocebus patas).
1974
Gyr K. | Wolf R.H. | Felsenfeld O.
Immunohistochemical study on the atretic and the growing follicles after experimental superovulation in rats 1. Number of follicles by superovulation.
1997
Kwak S.D.
Selenium toxicosis with focal symmetrical poliomyelomalacia in postweaning pigs in South Africa.
1996
Penrith M. L. | Robinson J.T.R.
Influence of time after the removal of nocodazole from nuclear donors on the development of reconstituted embryos in bovine nuclear transplantation.
1995
Tanaka H. | Takahashi Y. | Hishinuma M. | Kanagawa H.
The present study examined the influence of post-cleavage time of nuclear donors on the development of reconstituted embryos in bovine nuclear transfer. Blastomeres of 16-cell stage embryos derived from in vitro-maturation, fertilization and culture were used as nuclear donor source. They were treated with 10 mu-M nocodazole for 12 hr. Blastomeres that cleaved within 3 hr after the removal of nocodazole were used-for the study. Metaphase II (M-II) oocytes were used as recipient cytoplasm. IN experiment 1, donor blastomeres at 6, 11 and 15 hr after the removal of nocodazole and donor blastomeres no treated with nocodazole were transferred into ethanol-exposed and enucleated oocytes. The reconstituted embryos produced by donor blastomeres oat 6 hr after the removal of nocodazole had a significantly higher developmental rate to the blastocyst stage than those at 15 hr and the untreated groups (P<0.01). In experiment 2, blastomeres at 6 hr after the removal of nocodazole used as nuclear donors were transferred into ethanol-exposed and enucleated M-II oocytes. The reconstituted embryos with ethanol-exposed and enucleated oocytes as recipient cytoplasm had a significantly higher rate of initial-cleavage (P<0.05) and development to the blastocyst stage (P<0.01) than non ethanol-exposed and enucleated M-II oocytes. These results demonstrate that the development of reconstituted embryos was improved when cleaved donor blastomeres after the removal of nocodazole were immediately transferred (at 3-6 hr post-cleavage) into activated enucleated oocytes by exposure to ethanol.
Mostrar más [+] Menos [-]Study on prevention of adhesion formation by use of sodium carboxymethylecellulose and dextran 70-(1)-Adhesion formation by artificial injuries and its preventive effect of sodium carboxymethylcellulose and dextran 70 in rabbits.
1994
Lee H.J. | Choi M.C. | Kang T.Y. | Park C.S.
Effects of histamine on the ruminal smooth muscle motility of cattle.
1994
Yoon B.C. | Han H.J. | Han B.K.
The characters of Ca2+ activated Cl- channel and its role in the cardiac myocytes.
1994
Park C.O. | Kim Y.M. | Haan J.H. | Hong S.G.
Activity and application of 20alpha-hydroxysteroid dehydrogenase in rat-(2)-Changes in activities of the splenic macrophages and ovarian histological findings.
1994
Kang C.B. | Kwak S.D.
Anaplasmosis in imported deer-The need for stringent regulatory guidelines.
1994
Baek B.K. | Jung J.M. | Kim N.S. | Son K.R. | Byon S.Y. | Kakoma I.
