Objective: This study aimed to establish and focus on the implications of precise energy and protein levels on reproductive performance. This study based on field facts showed that exces¬sive feed intake, especially protein intake, to increase productivity will lead to some decreased reproductive efficiency through disruption of reproductive hormonal compound biosynthesis or increasing blood urea nitrogen (BUN), which has rarely been studied. Materials and Methods: Healthy and mature Saanen crossbred goats raised by traditional farm¬ers (2–4 years old; ± 45 kg) were divided into three experimental groups [T0 = basal feed according to the feed provided by farmers, T1 = protein and energy balanced feed according to National Research Council (NRC) (±5%), T2 = feed >20% excess protein, and excess energy from NRC] (n = 75). The factorial method was used in the experimental design with a post-hoc least significant difference test. Results: The data showed a significant difference (p < 0.05) in the biochemical or hormonal parameters between the control and the treatment groups. Also, T1 showed leads in any blood profile and reproductive hormone parameters such as total protein (79.6 ± 5.3 gm/dl), cholesterol (3.26 ± 0.47 mmol/l), progesterone (1.79 ± 2.21 ng/ml), and estrogen (47.85 ± 5.51 pg/ml), as well as the lowest levels of BUN (11.6 ± 1.59 mmol/l) and cortisol (25.07 ± 14.85 ng/ml) levels. T1 had the highest reproductive potential after treatment. Conclusion: The adverse effects of high and low protein consumption on reproductive hormones seem to be related to the blood profile and ovarian function, as hormone concentrations change significantly and lead to reproductive impairment. The data showed that balanced nutrient levels (5%/T1) resulted in excellent blood and hormone parameters. [J Adv Vet Anim Res 2023; 10(2.000): 257-268]

Objectives: This study aimed to examine the impacts of the wide range of concentrations of glu¬cose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen. Materials and Methods: The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF + glucose (75, 150, 450, and 900 mm), and TCEF + trehalose (75, 150, 450, and 900 mm). After equilibrations, the semen straws were frozen under LN2 in the LN2 tank. After LN2 storage, the straws were thawed at 37°C for 30 seconds. The sperm parame¬ters of all study groups were checked after equilibration and freezing. Results: After equilibration, the progressive motility (PM), total motility (TM), and viability of sperm in G-75, G-150, G-450, T-75, T-150, and T-450 were not significantly different (p < 0.05) from those in control. After cryopreservation and thawing, the PM, TM, and plasma membrane integrity (PMI) of T-150 were significantly higher (p < 0.05) than in control, G-75, G-900, T-75, and T-900. The viability of sperm in T-150 was substantially higher (p < 0.05) than in the control, whereas there was no significant difference among the control, G-75, G-900, T-75, and T-900. However, the acrosome integrity (AI) of sperm in G-900 was significantly decreased (p < 0.05) compared to the control, G-75, G-150, G-450, T-75, T-150, and T-450. Conclusion: According to the findings, the supplementation of 150 mm trehalose in the TCEF dilu¬ent was more efficient for sperm cryopreservation in the buck as reflected by PM, TM, viability, PMI, and AI. [J Adv Vet Anim Res 2023; 10(2.000): 169-177]

Objective: The study aimed to investigate the hepatoprotective effects of Gastrodia elata rhi¬zome (GR) on thioacetamide (TAA)-induced liver injury in dogs. We evaluated serum biochemical and hematological parameters, with emphasis on alanine transaminase (ALT), alanine phosphates (ALP), and nitric oxide (NO) levels, in dogs with TAA-induced liver injury. Materials and Methods: The animals were divided into a control group (Con), TAA group, Silymarin group (Sil, 50 mg/kg), Gastrodia rhizome low dose (GRL) (low) + TAA, GRH (high) + TAA, and GR high-dose group (GRH) control group. GRL and GRH were given daily at 50 and 100 mg/kg, respectively. TAA was given on days 1, 4, and 7 at a dose of 300 mg/kg. Results: GR significantly reduced liver injury in treated animals, as indicated by lowered levels of ALT (about 32% at day 21 in both GRL + TAA and GRH + TAA groups), ALP (about 17% and 21% at day 21 in both GRL + TAA, GRH + TAA groups, respectively), and NO (about 36% at day 21 in both GRL + TAA, GRH + TAA groups) compared to the TAA control group. Hematological parameters showed mild changes during the experiment. High-performance liquid chromatography analysis revealed gastrodin, a major component of the GR extract, constitutes 2.6% of the extract. Conclusion: The GR demonstrated significant hepatoprotective effects against TAA-induced liver injury in dogs. The study provides evidence for the potential therapeutic use of GR in the man¬agement of liver diseases. [J Adv Vet Anim Res 2023; 10(2.000): 144-150]

Objective: Arcobacter butzleri, Listeria monocytogenes, Staphylococcus aureus, and Campylobacter jejuni are significant foodborne pathogens regarding the consumption of raw poultry meat. An existing survey was conducted to assess the occurrence of S. aureus, C. jejuni, A. butzleri, and L. monocytogenes in raw poultry meat samples. Materials and Methods: Ninety-four raw ostrich, turkey, chicken, and quail meat samples were collected and subjected to culture-based analysis. Staphylococcus aureus, C. jejuni, A. butzleri, and L. monocytogenes isolates were confirmed by standard biochemical techniques. Results: The occurrence of A. butzleri, C. jejuni, L. monocytogenes, and S. aureus in poultry meat samples was 11.45%, 17.70%, 1.04%, and 16.66%, respectively. L. monocytogenes was absent in chicken, turkey, and ostrich meat samples. Only one quail meat (4.16%) was positive for L. monocytogenes. The uppermost contamination rate with A. butzleri, C. jejuni, and S. aureus was found in chicken (25%), turkey (25%), and turkey (25%) meat samples, respectively. The concur¬rent occurrence of A. butzleri + C. jejuni + S. aureus bacteria amid the examined poultry meat samples was 2.08%. Conclusion: This is an initial report of A. butzleri, S. aureus, C. jejuni, and L. monocytogenes in poultry meat samples. Adequate cooking of poultry meat can diminish foodborne diseases due to A. butzleri, S. aureus, L. monocytogenes, and C. jejuni bacteria, and these species may constitute a public health problem. [J Adv Vet Anim Res 2023; 10(2.000): 205-210]

Objective: The study investigated the gut content and recorded morphometric and hematological parameters in the Eurasian collared dove (Streptopelia decaocto). Materials and Methods: 24 samples of healthy birds (12 from each sex) were collected from different wetlands in Punjab, Pakistan, from December 2022 until February 2023. Birds were cap¬tured live for blood samples, morphometric, and gut analyses. Results: The current study revealed that mensural measurements showed no significant differences in all parameters except tail length, which was significantly longer in males (14.59 ± 0.30) compared to females (12.88 ± 0.43). Hematological parameters were hemoglobin, 23.95 gm/dl; red blood cells, 3.97 × 106/μl; white blood cells, 429.9.67 × 103/μl; hematocrit, 72.14%; mean cor¬puscular volume, 183.24 FL; mean corpuscular hemoglobin, 61.70 pg; mean corpuscular hemo¬globin concentration, 32.37 pg; platelets, 7.01/μl; and red cell distribution width, 110.86/μl. The percentages of neutrophils, lymphocytes, monocytes, and eosinophils were 71.33%, 23.03%, 3.30%, and 1.43%, respectively. The gut content of the Eurasian collared dove mainly consisted of rice, wheat, corn, and millet seeds. Some stony materials were also present. Conclusion: Our study concluded that male and female Eurasian collared doves are alike in biometrics (except tail length) and hematological profiles. Gut content and weight were also similar. Males were slightly larger than females. The gut content showed that the Eurasian collared dove mainly feeds on rice, wheat, corn, and millet seeds. In this study, hematological parameters were also studied. [J Adv Vet Anim Res 2023; 10(2.000): 244-248]

Objective: This study aimed to examine the concentration of nonessential amino acids (NEAAs) in ruminant feed in tropical areas, with a focus on forage grasses and legumes in Indonesia. Materials and Methods: A total of 11 grasses (Chrysopogon zizanioides, Brachiaria brizantha, Brachiaria humidicola, Paspalum dilatatum, Paspalum atatum, Chloris gayana, Pennisetum polystachion, Panicum maximum, Cenchrus biflorus, Andropogon canaliculatus, and Digotaria decumbens) and six legumes (Arachis hypogaea, Pueararia Javanica, Centrosema pubescens, Clitoria ternatea, and Arachis pintoi) were analyzed for NEAA content using high-performance liquid chromatography (HPLC). Results: Based on the results of this research, it was found that almost all NEAA content in forage was less than 3% (Serine (Ser), Alanine (Ala), Glycine (Gly), Tyrosine (Tyr), Proline (Pro), Cysteine (Cys), and Asparagine (Asn), except for glutamic (Glu) acid and arginine (Arg) in some legumes. The Glu content in grass ranges from 0.76% to 2.61%, and the Arg content ranges from 0.92% to 2.09%. These two NEAAs were most abundant in grasses and legumes, with concentrations of 5.10% to 6.27% and 3.10% to 5.53%, respectively. Conclusion: Our study concluded that Glu and Arg were the most abundant NEAAs in tropical forages in Indonesia, with legumes having a higher concentration of NEAAs compared to grasses. Among the legumes, A. hypogaea had the highest NEAA content (23.40%), while among the forages, C. zizanioides had the highest NEAA content (12.37%). However, it was observed that neither legumes nor grasses could fulfill the metabolizable TNEAAs requirements for gaining cattle (250 kg of empty body weight gain), unlike commercial concentrates, which were found to meet the requirements, especially for Arg, Glu, and thyronine. The provision of concentrate is necessary to supplement forage to meet the NEAA needs for cattle. [J Adv Vet Anim Res 2023; 10(4.000): 820-829]

Objective: Protein and carbohydrates are substances needed by ruminants, especially sheep. Providing protein and carbohydrates must pay attention to their degradation. In addition, balancing nutrients to meet the nutritional needs of rumen microbes is very important because the unbalanced availability of rumen degradable protein (RDP) and readily available carbohydrate (RAC) at one time can cause suboptimal microbial protein synthesis efficiency. Materials and Methods: Completely randomized design with a nonfactorial pattern of five treatments with three replications. Treatment includes RDP and RAC ratios, namely R1 = 2.30, R2 = 2.00, R3 = 1.70, R4 = 1.50, and R5 = 1.30. Results: The results showed that the balance of RDP and RAC in sheep ration formulation in vitro had a very significant effect (p < 0.01) on NH3, microbial protein synthesis, total gas, total microbes, and organic matter digestibility (OMD) but had no significant effect (p > 0.05) on rumen pH and dry matter digestibility (DMD). Conclusion: The optimal balance of RDP and RAC in the formulation of sheep rations in vitro was obtained at a ratio of 2.30 with NH3 (mM) 8.47, rumen pH 5.97, microbial protein synthesis (mg/100 ml) 123, gas (ml/g of material) 145, total microbes (cells/ml) 2.012 × 106, (log CFU cells/ml) 6.3025, DMD 61.0%, and OMD 63.1%. [J Adv Vet Anim Res 2023; 10(4.000): 738-743]

Objective: This study evaluated the utilization of marigold leaves (MGLs) in rations and their impact on rumen enzyme activity, fermentation parameters, methane (CH4) emission, and nutrient digestibility in vitro. Materials and Methods: The experimental diets comprised different proportions of MGL incorporated into the dry matter (DM) rations. Experimental design: The MGL treatments in diets include 0% (MGL-0), 7% (MGL-7), and 14% (MGL-14). Results: Results indicated that MGL-14 substantially raised (p < 0.05) the rumen parameters, including NH3–N and microbial protein, total volatile fatty acids, acetate (C2), propionate (C3), butyrate (C4), and the C2:C3 ratio. In contrast, the MGL-7 and MGL-14 groups experienced a noteworthy reduction (p < 0.05) in the total protozoa population. The MGL-7 and MGL-14 treatments also led to a substantial increase in the digestibility of DM, organic matter (OM), and crude fiber (CF) in the rumen. However, they also resulted in a decline (p < 0.05) in crude protein (CP) digestibility. The DM and OM total digestibilities were higher (p < 0.05) in the MGL-14 and MGL-7 groups. The utilization of MGL did not influence (p > 0.05) the ruminal enzyme activities (carboxymethyl cellulase, amylase, protease), cumulative gas production, kinetics, ruminal pH value, CH4 and CO2 production, total CF, and CP digestibility. Conclusion: The utilization of MGL until 14% DM in diets can enhance ruminal fermentation parameters and nutrient digestibility in vitro without negatively affecting gas production kinetics or ruminal enzyme activities. However, it did not have any impact on CH4 production. [J Adv Vet Anim Res 2023; 10(4.000): 782-793]

Objective: This study investigated the effectiveness of different physical forms of feed containing Ipomoea aquatica waste and concentrate feed on the rumen characteristics, chewing activity, and performance of sheep. Materials and Methods: Twenty-four rams (19.87 ± 2.19 kg) were arranged in a completely randomized design. Rams were fed dried I. aquatica waste and concentrate feed provided separately (RCF) (conventional feeding system), and total mixed ration consisted of mash complete feed (MCF), and pelleted complete feed (PCF). The data were analyzed using a one-way analysis of variance. Results: The dry matter intake (DMI), average daily gain, and feed conversion ratio of rams fed different physical forms of feed containing I. aquatica waste in their diet were similar between the treatments, ranging from 4.08% to 4.29% of body weight, 120–180 gm, and 6.32–9.17, respectively. Different physical feeds did not affect microbial synthesis in the rumen. Methane emissions per unit of production were similar between the groups. The PCF sheep ate faster (0.24 min/gm DMI) than the MCF sheep (0.38 min/gm DMI), but similar to the RCF sheep (0.31 min/gm DMI). Conclusion: Dried I. aquatica waste was useful as an alternative lamb feed roughage during the dry season. The pelleted mixed ration was more efficient than mash in increasing DMI. The dried I. aquatica waste was environmentally friendly for mitigating enteric methane emissions by sheep. [J Adv Vet Anim Res 2023; 10(4.000): 677-684]

Objective: This research aimed to evaluate potential tropical seaweed from Indonesia as an ingredient or supplement feed for ruminants based on chemical composition and in vitro rumen fermentation parameters. Materials and methods: The seven natural tropical seaweeds (three green and four red species) were collected from Ndrini and Sepanjang Beach, Gunungkidul, Yogyakarta, Indonesia. The experimental design on secondary metabolite profiles used a completely randomized design, and the in vitro gas production test used a randomized complete block design with seven seaweed species variances and four replications (blocks) based on rumen fluid collection time. The data obtained was analyzed using analysis of variance (ANOVA), and Duncan‘s Multiple Range Test was used to test the variation in the analysis. Results: The seven tropical seaweed species have potential as mineral sources for ruminants, except for macromineral (P and S) and micromineral (Cu). The red tropical seaweed has potential as a protein source (Gelidium spinosum (S.G.Gmelin) P.C. Silva, Hypnea pannosa, and Acanthopora muscoides (L.) Bory), and the green seaweed (Chaetomorpha linum (O.F. Mull.) Kutz and Cladopora sp.) has potential as a crude fiber (CF) source for ruminants. As indicated by secondary metabolites and gas production in vitro, the green species (C. linum (O.F. Mull.) Kutz and Enteromorpha compressa) and red species (A. muscoides (L.) Bory and Gelidium amansii (J.V. Lamouroux) J.V. Lamouroux) could be degraded in the rumen and had quite high phenolic compounds. Conclusion: The seven tropical seaweed species have the potential to be an ingredient or supplement feed for ruminants, and there were four species that have the potential to reduce methane emissions. [J Adv Vet Anim Res 2023; 10(4.000): 751-762]