Objectives: The research was carried out on broilers to determine the efficacy of probiotics (Bacillus subtilis and Saccharomyces boulardii combined) supplementation on growth performances, nutrient retention (metabolizable energy, dry matter, and crude protein), and cecal microbiology (Bifidobacteria spp., Clostridium spp., and coliforms). Materials and Methods: A total of 160 broiler chicks (day-old) were selected and differentiated randomly into 4 groups (T0, T1, T2, and T3) (40 × 4) comprising 40 birds in every single group. The control group (T0) was fed commercial broiler feed only and the other three groups, referred to as treatment groups (T1, T2, and T3), were treated with 1 gm ciprofloxacin, 1 gm probiotic, and 1 gm probiotic plus 0.5 gm enzyme, respectively, in per liter of fresh dietary water 8 h daily for 7 days in each phase. Experimental trials were divided into 2 phases, the starter phase from day 0 to 21 and the finisher phase from day 22 to 35. Results: Bodyweight gain and nutrient retention in experimental broiler birds in treatment groups were significantly (p < 0.05) higher than the control group. Overall body weight gain and nutrient retention of broiler chicks in treatment groups T2 and T3 were better than T1. From day 22 to 35, cecal Clostridium and coliform bacterial load counts were significantly lower p < 0.01, p < 0.05, and p < 0.01, respectively, in T1, T2, and T3 treatments than T0. Overall, Clostridium and coliform bacterial counts in the birds of treatment group T2 were significantly lower (p < 0.05) than T0. Conclusion: The probiotics, in addition to enzyme supplementation, had suitable influence effects on growth performance of broilers, birds retention of nutrient, and microfloral count in birds cecum. [J Adv Vet Anim Res 2021; 8(4.000): 534-539]

Objective: The effect of seasonality needs to be considered in designing future studies because global warming has caused a rise in ambient temperatures. The objective of the present study is to investigate the effect of high ambient temperatures on fecal score and fecal microflora in dairy cows during summer. Materials and Methods: During the 7 days before the sampling of feces, the daily mean tempera¬tures were 19.9°C in early summer and more than 27.5°C in late summer. Fecal samples were collected from the rectum of cows and the fecal score was evaluated on a 4-point scale. The equalized samples were used to extract the genomic deoxyribonucleic acid (DNA) of the bacteria (Escherichia coli, Salmonella, Lactobacillus, and Bifidobacterium). Results: There was no significant difference in fecal scores between the sampling times in early and late summer. In the populations of the bacteria, there was no significant difference between sampling days in the DNA level of Salmonella, and E. coli in late summer increased to more than three times the level in early summer. However, both levels of Lactobacillus and Bifidobacterium in early summer significantly decreased after 2 months. Conclusion: These data suggest that the increase in temperature in late summer may adversely affect the populations of bacteria in the intestinal environment of dairy cows. In addition, the method used in the present study was sufficient to evaluate the changes in internal and external environmental conditions of dairy cattle. [J Adv Vet Anim Res 2021; 8(2.000): 307-311]

Objective: This research aims to study the target specificity of selective bioactive compounds in complexing with the human angiotensin-converting enzyme (hACE2) receptor to impede the severe acute respiratory syndrome coronavirus 2 influx mechanism resulting in cardiac injury and depending on the receptors active site properties and quantum tunneling. Materials and Methods: A library of 120 phytochemical ligands was prepared, from which 5 were selected considering their absorption, distribution, metabolism, and excretion (ADMET) and quantitative structureactivity relationship (QSAR) profiles. The protein active sites and belong¬ing quantum tunnels were defined to conduct supramolecular docking of the aforementioned ligands. The hydrogen bond formation and hydrophobic interactions between the ligandrecep¬tor complexes were studied following the molecular docking steps. A comprehensive molecular dynamic simulation (MDS) was conducted for each of the ligandreceptor complexes to figure out the values root mean square deviation (RMSD) (Å), root mean square fluctuation (RMSF) (Å), H-bonds, Cα, solvent accessible surface area (SASA) (Å2), molecular surface area (MolSA) (Å2), Rg (nm), and polar surface area (PSA) (Å). Finally, computational programming and algorithms were used to interpret the dynamic simulation outputs into their graphical quantitative forms. Results: ADMET and QSAR profiles revealed that the most active candidates from the library to be used were apigenin, isovitexin, piperolactam A, and quercetin as test ligands, whereas serpentine as the control. Based on the binding affinities of supramolecular docking and the parameters of molecular dynamic simulation, the strength of the test ligands can be classified as isovitexin > quercetin > piperolactam A > apigenin when complexed with the hACE2 receptor. Surprisingly, serpentine showed lower affinity (−8.6 kcal/mol) than that of isovitexin (−9.9 kcal/mol) and quer¬cetin (−8.9 kcal/mol). The MDS analysis revealed all ligands except isovitexin having a value lower than 2.5 Ǻ. All the test ligands exhibited acceptable fluctuation ranges of RMSD (Å), RMSF (Å), H-bonds, Cα, SASA (Å2), MolSA (Å2), Rg (nm), and PSA (Å) values. Conclusion: Considering each of the parameters of molecular optimization, docking, and dynamic simulation interventions, all of the test ligands can be suggested as potential targeted drugs in blocking the hACE2 receptor. [J Adv Vet Anim Res 2021; 8(4.000): 540-556]

Objective: In developing countries, such as Bangladesh, the live bird market (LBM) is a vital location for the trading of live poultry. The study was carried out in nine LBMs located around Bangladesh to ascertain the present regulations and procedures governing their operation. Additionally, the responsibilities and levels of engagement of the stakeholders were determined. Materials and Methods: The data were gathered through the use of a semi-structured interview guide. Thematic analysis was used to code the interview transcripts iteratively. Results: The findings indicated that the government was directly and indirectly involved in the leasing process of the markets. A market in this country is divided into numerous sectors, including LBM, fish market, vegetable market, and grocery stores. A markets hygienic condition is highly dependent on market authoritys decisions. In some markets, market officials conducted routine sanitary inspections. Veterinarians played a little role in the inspection procedure. Conclusion: There is no adequate, functional monitoring system to ensure that LBMs adhere to cleanliness and adequate and functional biosecurity. Biosecurity enhancements, effective cleaning programs, and regular monitoring by relevant authorities are critical for LBMs in Bangladesh. [J Adv Vet Anim Res 2021; 8(4.000): 671-678]

Objective: The influence of the degree of pigmentation, shine, weight, and form index on the hatchability rate of quail eggs (Coturnix coturnix japonica) was examined. Materials and Methods: Three thousand three hundred eggs were incubated after they were classified according to the degree of shell pigmentation: high (HP), medium (MP), and low (LP); shell shine: shiny (SS), and opaque; Egg weight: 13 gm; form index: 78%. The MannWhitney non-parametric test for independent samples was used to evaluate hatching data. Results: Hatchability was best in eggs with HP and SS (p < 0.05): 69.2% and 75.7%, respectively; eggs with MP had the lowest hatchability rate (63.1%). The highest risk of embryo death (17.8%) was observed in eggs containing LP (p < 0.05). Eggs weighing between 12 and 12.9 gm had the highest hatchability rate (p < 0.05) (79.11%). Eggs with a form index >78% had the highest hatch¬ability rate (p < 0.05) (67.62%). Conclusions: HP, MP shell eggs, and SS eggs with a weight of between 12.0 and 12.9 gm and a shape index of greater than 78% have a higher hatchability rate. [J Adv Vet Anim Res 2021; 8(4.000): 629-634]

Objective: To evaluate the influence of the unsaturated to saturated ratio of fatty acids (FAs) reaching the duodenum on postruminal digestion of FAs, mainly focused on stearic acid (C18:0). Materials and Methods: Six Holstein steers [208 ± 3 kg initial live weight (LW)] with cannulas in the abomasum and proximal duodenum were used in a replicated 3 × 3 Latin square design. Steers were fed a fixed amount of a basal steam-flaked corn-based diet containing 8% supple mental fat and were daily infused via abomasum with 0, 67, and 165 gm oleic acid (C18:1). The experiment lasted for 42 days. Results: The daily total FA (TFA) intake (dietary FA intake plus abomasal infusion of oleic acid) represented a 1.78, 2.10, and 2.56 gm TFA/kg LW ratio. The unsaturated to saturated ratio of FAs entering the duodenum increased (p < 0.01) aslevel C18:1 infusion into the abomasum increased. Infusion of C18:1 tended (quadratic component, p = 0.07) to improve postruminal TFA digestion, being maximal for the 67 gm/day infusions. This increase in TFA digestion was due to increased (quadratic component, p = 0.03) postruminal C18:0 digestion (postruminal digestion of the other FAs was not different, p ≥ 0.13). Conclusion: Increasing the unsaturated to saturated ratio of FAs entering the small intestine will enhance intestinal C18:0 digestion. This positive effect is expected to be more likely bene ficial when FA intake is high (and thus, the duodenal flow of FA is high), but this benefit looks diminished when the quantity of TFA reaching the intestine exceeds the proportion of 2.13 gm FA/kg LW. [J Adv Vet Anim Res 2021; 8(3.000): 465-470]

Objective: This work investigated the antimicrobial resistance (AMR) and virulence of Escherichia coli and Staphylococcus aureus in communally consumed cheeses in Egypt. Materials and Methods: This study examined 100 samples of Domiati, Tallaga, Cheddar, and Ras cheese collected from several shops and supermarkets. Samples were spread on selective media to isolate bacterial strains. Molecular characterization of bacterial isolates was carried out using polymerase chain reaction to determine Shiga toxin 1 (stx1), Shiga toxin 2 (stx2), eaeA, and nuc genes. The isolates were tested for susceptibility to 14 antibiotics by disk diffusion assay. Results: In this study, several E. coli serotypes were identified. E. coli O26:H11, O103:H2, and O111:H2 expressed stx1/2, E. coli O114:H4 expressed stx1, E. coli O17:H18, O21:H7 and O146:H21 expressed stx2, while only E. coli O26:H11 and O111:H2 expressed eaeA. The E. coli isolates were resistant to at least one antibiotic, while most isolates (82.4%) showed multidrug resistance (MDR). AMR to erythromycin was the highest (100%), followed by nalidixic acid (94.1%), cefotax¬ime (82.4%), vancomycin and cephalothin (64.7%), penicillin G (52.9%), sulfamethoxazole (47.1%), amikacin and kanamycin (35.3%), ampicillin (29.4%), tetracycline and ciprofloxacin (23.5%), and doxycycline (11.8%), while gentamicin showed the least resistance (5.9%). The multiple antibi¬otic resistance (MAR) index of the isolated E. coli ranged from 0.071 to 1 (mean = 0.478). All S. aureus isolates expressed the nuc gene and demonstrated resistance to at least one antibiotic, and 90% of isolates were MDR. AMR to kanamycin and cephalothin was the highest (100%), fol¬lowed by penicillin (90%), doxycycline (70%), nalidixic acid and sulfamethoxazole (60%), erythro¬mycin (50%), tetracycline, cefotaxime, and gentamicin (40%), ciprofloxacin and ampicillin (30%), and amikacin (20%). In comparison, vancomycin showed the least resistance (10%). MAR index of isolated S. aureus ranged from 0.143 to 1 (mean = 0.529). Conclusion: The antimicrobial-resistant E. coli and S. aureus are potential risks for public health and may have a role in disseminating AMR to other pathogenic and non-pathogenic microbes. [J Adv Vet Anim Res 2021; 8(2.000): 246-255]

This review attempted to explain factors associated with negative energy balance (NEB) occur¬ring during the periparturient period in dairy cows raised under tropical climatic conditions. The NEB has long been proven as an inevitable event in periparturient dairy cows. This condition had negative effects on the overall performances of dairy cows, including milk production, reproduc¬tion, and health condition. Therefore, periparturient management to overcome the NEB problem is vital for optimizing profit in dairy farming. In most tropical countries such as Thailand, dairy cows have been predominantly kept by small-holder farmers. Consequently, baseline milk yields, feed availability, feeding management, and general farming practices are different from typical commercial dairy farming. Heat stress also plays a crucial role in NEB conditions, and elevated temperature-humidity indexes above-normal conditions are recorded throughout the year. These factors influence the NEB in tropical dairy cows, which could result in different outcomes and consequences. Understanding the affecting components of NEB in dairy cows would help alleviate the severity of the NEB and its consequences, optimizing the dairy cows performance. [J Adv Vet Anim Res 2021; 8(3.000): 378-387]

Objective: Anticoccidial drugs may lead to the development of drug resistance and drug residues. Herbal extracts could be an attractive alternative. This research was undertaken to evaluate the anticoccidial outcome of Carica papaya compared with the anticoccidial drug (Toltazuril) in Sonali chickens. Materials and Methods: A total of 80 Sonali chickens were evenly and equally allocated into four groups, namely T1 (non-infected control), T2 (infected control), T3 (treated with C. papaya), and T4 (treated with Toltrazuril). All groups were experimentally infected with oocysts of mixed Eimeria spp. orally except T1, and the mixed Eimeria spp. oocyst load (OL), body weight (BW) gain, and hematological parameters were calculated. Results: In the findings, the highest OL reduction rate in T4 was 100%, while the T3 was 83.44%. Nevertheless, BW differed significantly (p < 0.01) among the different groups, while the daily BW gain was higher in T3 amounting to 8.10 gm. In the case of hematological parameter, total eryth¬rocyte count (TEC), hemoglobin (Hb), packed cell volume (PCV), and erythrocyte sedimentation rate in different groups were almost the same and were also statistically insignificant (p > 0.05) barring total leukocyte count resulting as significant (p < 0.05) at day 30. Additionally, the results of Pearsons correlation in T3 at day 30 indicated a strong significant (p < 0.01) negative correlation between OL and BW (r = −0.780) with the following regression equation: y = −0.16*x + 433.665. Moreover, the correlation of TEC, PCV, and Hb with OL was significantly (p < 0.01) negative, r = −0.786, r = −0.752 and r = −0.633, where the regression equations were y = −0.03*x + 4.51, y = −0.03*x + 27.42, and y = −0.04*x + 11.40, respectively. Conclusion: Long-term use of C. papaya leaves extracts effectively controls coccidiosis in Sonali chickens and can act as an effective growth promoter. [J Adv Vet Anim Res 2021; 8(1.000): 91-100]

Objective: This experiment was undertaken to assess the scope and cost-effectiveness of the fer¬mented corn mixture (FCM)-based buffalo fattening approach compared to urea molasses straw (UMS) and silage-based approach. Materials and Methods: A completely comparative randomized design experiment was con¬ducted for 90 days with three treatments and five buffalo bulls in each. UMS, silage, and FCM roughage-based fattening diets were attributed as T1, T2, and T3, respectively. Two types of protein supplements, i.e., Type 1 (Bangladesh Livestock Research Institute-developed) for T1 and T2 and Type 2 (prescribed by farmers) for T3 treatments, were used. All the parameters were analyzed through Statistical Package for the Social Sciences, 20 software. Results: Dry matter intake (kg, %live weight) was significantly higher in T1 (2.65), followed by T2 (2.34) and T3 (2.00), respectively. The crude protein intake, digestible crude protein intake, and digestible dry matter intake (kg/d) significantly (p < 0.05) differed between T1 and T3, but not T2. The digest¬ibility of acid detergent fiber (65.97) was significantly higher for T3 than T1 and T2 (54.44 and 58.73, respectively). Neutral detergent fiber digestibility of T3 (70.35) also differed (p < 0.05) with T1 (60.97) but not T2 (64.78). No difference was observed in the case of growth, but feed conversion ration was found to be significantly (p < 0.05) better in T2 (7.10) than T1 (8.35), where T3 (7.24) was neutral. The significantly (p < 0.001) highest expense [216.37 Bangladesh taka (BDT)/kg gain] was required for T1, followed by T2 and T3 (174.47 and 126.33 BDT/kg gain, respectively). Net profit from T3 and T2 (15,877 and 15,175 BDT, respectively) gained significantly (p < 0.05) higher than T1 (11,265 BDT). Conclusion: The FCM-based diet was suitable and cost-effective as a buffalo fattening approach. [J Adv Vet Anim Res 2021; 8(2.000): 195-202]